Context-Dependent Regulation of Conjunctival Goblet Cell Function by Allergic Mediators

Abstract In the eye, goblet cells responsible for secreting mucins are found in the conjunctiva. When mucin production is not tightly regulated several ocular surface disorders may occur. In this study, the effect of the T helper (Th) 2-type cytokines IL4, IL5, and IL13 on conjunctival goblet cell f...

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Autores principales:	Laura García-Posadas, Robin R. Hodges, Yolanda Diebold, Darlene A. Dartt
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/0cf80d37144944938217eb5a2176f34d
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spelling	oai:doaj.org-article:0cf80d37144944938217eb5a2176f34d2021-12-02T11:40:16ZContext-Dependent Regulation of Conjunctival Goblet Cell Function by Allergic Mediators10.1038/s41598-018-30002-x2045-2322https://doaj.org/article/0cf80d37144944938217eb5a2176f34d2018-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-30002-xhttps://doaj.org/toc/2045-2322Abstract In the eye, goblet cells responsible for secreting mucins are found in the conjunctiva. When mucin production is not tightly regulated several ocular surface disorders may occur. In this study, the effect of the T helper (Th) 2-type cytokines IL4, IL5, and IL13 on conjunctival goblet cell function was explored. Goblet cells from rat conjunctiva were cultured and characterized. The presence of cytokine receptors was confirmed by Reverse Transcription-Polymerase Chain Reaction (RT-PCR). Changes in intracellular [Ca2+], high molecular weight glycoconjugate secretion, and proliferation were measured after stimulation with Th2 cytokines with or without the allergic mediator histamine. We found that IL4 and IL13 enhance cell proliferation and, along with histamine, stimulate goblet cell secretion. We conclude that the high levels of IL4, IL5, and IL13 that characterize allergic conjunctivitis could be the reason for higher numbers of goblet cells and mucin overproduction found in this condition.Laura García-PosadasRobin R. HodgesYolanda DieboldDarlene A. DarttNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-11 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Laura García-Posadas Robin R. Hodges Yolanda Diebold Darlene A. Dartt Context-Dependent Regulation of Conjunctival Goblet Cell Function by Allergic Mediators
description	Abstract In the eye, goblet cells responsible for secreting mucins are found in the conjunctiva. When mucin production is not tightly regulated several ocular surface disorders may occur. In this study, the effect of the T helper (Th) 2-type cytokines IL4, IL5, and IL13 on conjunctival goblet cell function was explored. Goblet cells from rat conjunctiva were cultured and characterized. The presence of cytokine receptors was confirmed by Reverse Transcription-Polymerase Chain Reaction (RT-PCR). Changes in intracellular [Ca2+], high molecular weight glycoconjugate secretion, and proliferation were measured after stimulation with Th2 cytokines with or without the allergic mediator histamine. We found that IL4 and IL13 enhance cell proliferation and, along with histamine, stimulate goblet cell secretion. We conclude that the high levels of IL4, IL5, and IL13 that characterize allergic conjunctivitis could be the reason for higher numbers of goblet cells and mucin overproduction found in this condition.
format	article
author	Laura García-Posadas Robin R. Hodges Yolanda Diebold Darlene A. Dartt
author_facet	Laura García-Posadas Robin R. Hodges Yolanda Diebold Darlene A. Dartt
author_sort	Laura García-Posadas
title	Context-Dependent Regulation of Conjunctival Goblet Cell Function by Allergic Mediators
title_short	Context-Dependent Regulation of Conjunctival Goblet Cell Function by Allergic Mediators
title_full	Context-Dependent Regulation of Conjunctival Goblet Cell Function by Allergic Mediators
title_fullStr	Context-Dependent Regulation of Conjunctival Goblet Cell Function by Allergic Mediators
title_full_unstemmed	Context-Dependent Regulation of Conjunctival Goblet Cell Function by Allergic Mediators
title_sort	context-dependent regulation of conjunctival goblet cell function by allergic mediators
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/0cf80d37144944938217eb5a2176f34d
work_keys_str_mv	AT lauragarciaposadas contextdependentregulationofconjunctivalgobletcellfunctionbyallergicmediators AT robinrhodges contextdependentregulationofconjunctivalgobletcellfunctionbyallergicmediators AT yolandadiebold contextdependentregulationofconjunctivalgobletcellfunctionbyallergicmediators AT darleneadartt contextdependentregulationofconjunctivalgobletcellfunctionbyallergicmediators
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Context-Dependent Regulation of Conjunctival Goblet Cell Function by Allergic Mediators

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