T Cell Immune Profiles of Blood and Tumor in Dogs Diagnosed With Malignant Melanoma

Investigation of canine T cell immunophenotypes in canine melanomas as prognostic biomarkers for disease progression or predictive biomarkers for targeted immunotherapeutics remains in preliminary stages. We aimed to examine T cell phenotypes and function in peripheral blood mononuclear cells (PBMC)...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Ellen E. Sparger, Hong Chang, Ning Chin, Robert B. Rebhun, Sita S. Withers, Hung Kieu, Robert J. Canter, Arta M. Monjazeb, Michael S. Kent
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	melanoma canine dog immune profile one health comparative oncology Veterinary medicine SF600-1100
Acceso en línea:	https://doaj.org/article/0cfee634520c4c68a85e229019c24a1f
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:0cfee634520c4c68a85e229019c24a1f
record_format	dspace
spelling	oai:doaj.org-article:0cfee634520c4c68a85e229019c24a1f2021-12-02T08:21:22ZT Cell Immune Profiles of Blood and Tumor in Dogs Diagnosed With Malignant Melanoma2297-176910.3389/fvets.2021.772932https://doaj.org/article/0cfee634520c4c68a85e229019c24a1f2021-12-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fvets.2021.772932/fullhttps://doaj.org/toc/2297-1769Investigation of canine T cell immunophenotypes in canine melanomas as prognostic biomarkers for disease progression or predictive biomarkers for targeted immunotherapeutics remains in preliminary stages. We aimed to examine T cell phenotypes and function in peripheral blood mononuclear cells (PBMC) and baseline tumor samples by flow cytometry, and to compare patient (n = 11–20) T cell phenotypes with healthy controls dogs (n = 10–20). CD3, CD4, CD8, CD25, FoxP3, Ki67, granzyme B, and interferon-γ (IFN-γ) were used to classify T cell subsets in resting and mitogen stimulated PBMCs. In a separate patient cohort (n = 11), T cells were classified using CD3, CD4, CD8, FoxP3, and granzyme B in paired PBMC and single cell suspensions of tumor samples. Analysis of flow cytometric data of individual T cell phenotypes in PBMC revealed specific T cell phenotypes including FoxP3+ and CD25+FoxP3- populations that distinguished patients from healthy controls. Frequencies of IFN-γ+ cells after ConA stimulation identified two different patient phenotypic responses, including a normal/exaggerated IFN-γ response and a lower response suggesting dysfunction. Principle component analysis of selected T cell immunophenotypes also distinguished patients and controls for T cell phenotype and revealed a clustering of patients based on metastasis detected at diagnosis. Findings supported the overall hypothesis that canine melanoma patients display a T cell immunophenotype profile that is unique from healthy pet dogs and will guide future studies designed with larger patient cohorts necessary to further characterize prognostic T cell immunophenotypes.Ellen E. SpargerHong ChangNing ChinRobert B. RebhunSita S. WithersHung KieuRobert J. CanterArta M. MonjazebMichael S. KentFrontiers Media S.A.articlemelanomacaninedogimmune profileone healthcomparative oncologyVeterinary medicineSF600-1100ENFrontiers in Veterinary Science, Vol 8 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	melanoma canine dog immune profile one health comparative oncology Veterinary medicine SF600-1100
spellingShingle	melanoma canine dog immune profile one health comparative oncology Veterinary medicine SF600-1100 Ellen E. Sparger Hong Chang Ning Chin Robert B. Rebhun Sita S. Withers Hung Kieu Robert J. Canter Arta M. Monjazeb Michael S. Kent T Cell Immune Profiles of Blood and Tumor in Dogs Diagnosed With Malignant Melanoma
description	Investigation of canine T cell immunophenotypes in canine melanomas as prognostic biomarkers for disease progression or predictive biomarkers for targeted immunotherapeutics remains in preliminary stages. We aimed to examine T cell phenotypes and function in peripheral blood mononuclear cells (PBMC) and baseline tumor samples by flow cytometry, and to compare patient (n = 11–20) T cell phenotypes with healthy controls dogs (n = 10–20). CD3, CD4, CD8, CD25, FoxP3, Ki67, granzyme B, and interferon-γ (IFN-γ) were used to classify T cell subsets in resting and mitogen stimulated PBMCs. In a separate patient cohort (n = 11), T cells were classified using CD3, CD4, CD8, FoxP3, and granzyme B in paired PBMC and single cell suspensions of tumor samples. Analysis of flow cytometric data of individual T cell phenotypes in PBMC revealed specific T cell phenotypes including FoxP3+ and CD25+FoxP3- populations that distinguished patients from healthy controls. Frequencies of IFN-γ+ cells after ConA stimulation identified two different patient phenotypic responses, including a normal/exaggerated IFN-γ response and a lower response suggesting dysfunction. Principle component analysis of selected T cell immunophenotypes also distinguished patients and controls for T cell phenotype and revealed a clustering of patients based on metastasis detected at diagnosis. Findings supported the overall hypothesis that canine melanoma patients display a T cell immunophenotype profile that is unique from healthy pet dogs and will guide future studies designed with larger patient cohorts necessary to further characterize prognostic T cell immunophenotypes.
format	article
author	Ellen E. Sparger Hong Chang Ning Chin Robert B. Rebhun Sita S. Withers Hung Kieu Robert J. Canter Arta M. Monjazeb Michael S. Kent
author_facet	Ellen E. Sparger Hong Chang Ning Chin Robert B. Rebhun Sita S. Withers Hung Kieu Robert J. Canter Arta M. Monjazeb Michael S. Kent
author_sort	Ellen E. Sparger
title	T Cell Immune Profiles of Blood and Tumor in Dogs Diagnosed With Malignant Melanoma
title_short	T Cell Immune Profiles of Blood and Tumor in Dogs Diagnosed With Malignant Melanoma
title_full	T Cell Immune Profiles of Blood and Tumor in Dogs Diagnosed With Malignant Melanoma
title_fullStr	T Cell Immune Profiles of Blood and Tumor in Dogs Diagnosed With Malignant Melanoma
title_full_unstemmed	T Cell Immune Profiles of Blood and Tumor in Dogs Diagnosed With Malignant Melanoma
title_sort	t cell immune profiles of blood and tumor in dogs diagnosed with malignant melanoma
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/0cfee634520c4c68a85e229019c24a1f
work_keys_str_mv	AT ellenesparger tcellimmuneprofilesofbloodandtumorindogsdiagnosedwithmalignantmelanoma AT hongchang tcellimmuneprofilesofbloodandtumorindogsdiagnosedwithmalignantmelanoma AT ningchin tcellimmuneprofilesofbloodandtumorindogsdiagnosedwithmalignantmelanoma AT robertbrebhun tcellimmuneprofilesofbloodandtumorindogsdiagnosedwithmalignantmelanoma AT sitaswithers tcellimmuneprofilesofbloodandtumorindogsdiagnosedwithmalignantmelanoma AT hungkieu tcellimmuneprofilesofbloodandtumorindogsdiagnosedwithmalignantmelanoma AT robertjcanter tcellimmuneprofilesofbloodandtumorindogsdiagnosedwithmalignantmelanoma AT artammonjazeb tcellimmuneprofilesofbloodandtumorindogsdiagnosedwithmalignantmelanoma AT michaelskent tcellimmuneprofilesofbloodandtumorindogsdiagnosedwithmalignantmelanoma
_version_	1718398526039261184

T Cell Immune Profiles of Blood and Tumor in Dogs Diagnosed With Malignant Melanoma

Ejemplares similares