Human iPS cell derived RPE strips for secure delivery of graft cells at a target place with minimal surgical invasion

Abstract Several clinical studies have been conducted into the practicality and safety of regenerative therapy using hESC/iPSC-retinal pigment epithelium (RPE) as a treatment for the diseases including age-related macular degeneration. These studies used either suspensions of RPE cells or an RPE cel...

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Autores principales:	Mitsuhiro Nishida, Yuji Tanaka, Yo Tanaka, Satoshi Amaya, Nobuyuki Tanaka, Hirofumi Uyama, Tomohiro Masuda, Akishi Onishi, Junki Sho, Satoshi Yokota, Masayo Takahashi, Michiko Mandai
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/0d0f6de5ad7845f5b720b0fcdc7f407c
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spelling	oai:doaj.org-article:0d0f6de5ad7845f5b720b0fcdc7f407c2021-11-08T10:54:39ZHuman iPS cell derived RPE strips for secure delivery of graft cells at a target place with minimal surgical invasion10.1038/s41598-021-00703-x2045-2322https://doaj.org/article/0d0f6de5ad7845f5b720b0fcdc7f407c2021-11-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-00703-xhttps://doaj.org/toc/2045-2322Abstract Several clinical studies have been conducted into the practicality and safety of regenerative therapy using hESC/iPSC-retinal pigment epithelium (RPE) as a treatment for the diseases including age-related macular degeneration. These studies used either suspensions of RPE cells or an RPE cell sheet. The cells can be injected using a minimally invasive procedure but the delivery of an intended number of cells at an exact target location is difficult; cell sheets take a longer time to prepare, and the surgical procedure is invasive but can be placed at the target area. In the research reported here, we combined the advantages of the two approaches by producing a quickly formed hiPSC-RPE strip in as short as 2 days. The strip readily expanded into a monolayer sheet on the plate, and after transplantation in nude rats, it showed a potency to partly expand with the correct apical/basal polarity in vivo, although limited in expansion area in the presence of healthy host RPE. The strip could be injected into a target area in animal eyes using a 24G canula tip.Mitsuhiro NishidaYuji TanakaYo TanakaSatoshi AmayaNobuyuki TanakaHirofumi UyamaTomohiro MasudaAkishi OnishiJunki ShoSatoshi YokotaMasayo TakahashiMichiko MandaiNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-14 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Mitsuhiro Nishida Yuji Tanaka Yo Tanaka Satoshi Amaya Nobuyuki Tanaka Hirofumi Uyama Tomohiro Masuda Akishi Onishi Junki Sho Satoshi Yokota Masayo Takahashi Michiko Mandai Human iPS cell derived RPE strips for secure delivery of graft cells at a target place with minimal surgical invasion
description	Abstract Several clinical studies have been conducted into the practicality and safety of regenerative therapy using hESC/iPSC-retinal pigment epithelium (RPE) as a treatment for the diseases including age-related macular degeneration. These studies used either suspensions of RPE cells or an RPE cell sheet. The cells can be injected using a minimally invasive procedure but the delivery of an intended number of cells at an exact target location is difficult; cell sheets take a longer time to prepare, and the surgical procedure is invasive but can be placed at the target area. In the research reported here, we combined the advantages of the two approaches by producing a quickly formed hiPSC-RPE strip in as short as 2 days. The strip readily expanded into a monolayer sheet on the plate, and after transplantation in nude rats, it showed a potency to partly expand with the correct apical/basal polarity in vivo, although limited in expansion area in the presence of healthy host RPE. The strip could be injected into a target area in animal eyes using a 24G canula tip.
format	article
author	Mitsuhiro Nishida Yuji Tanaka Yo Tanaka Satoshi Amaya Nobuyuki Tanaka Hirofumi Uyama Tomohiro Masuda Akishi Onishi Junki Sho Satoshi Yokota Masayo Takahashi Michiko Mandai
author_facet	Mitsuhiro Nishida Yuji Tanaka Yo Tanaka Satoshi Amaya Nobuyuki Tanaka Hirofumi Uyama Tomohiro Masuda Akishi Onishi Junki Sho Satoshi Yokota Masayo Takahashi Michiko Mandai
author_sort	Mitsuhiro Nishida
title	Human iPS cell derived RPE strips for secure delivery of graft cells at a target place with minimal surgical invasion
title_short	Human iPS cell derived RPE strips for secure delivery of graft cells at a target place with minimal surgical invasion
title_full	Human iPS cell derived RPE strips for secure delivery of graft cells at a target place with minimal surgical invasion
title_fullStr	Human iPS cell derived RPE strips for secure delivery of graft cells at a target place with minimal surgical invasion
title_full_unstemmed	Human iPS cell derived RPE strips for secure delivery of graft cells at a target place with minimal surgical invasion
title_sort	human ips cell derived rpe strips for secure delivery of graft cells at a target place with minimal surgical invasion
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/0d0f6de5ad7845f5b720b0fcdc7f407c
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Human iPS cell derived RPE strips for secure delivery of graft cells at a target place with minimal surgical invasion

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