Network Integration Analysis and Immune Infiltration Analysis Reveal Potential Biomarkers for Primary Open-Angle Glaucoma

Primary open-angle glaucoma (POAG) is a progressive optic neuropathy and its damage to vision is irreversible. Therefore, early diagnosis assisted by biomarkers is essential. Although there were multiple researches on the identification of POAG biomarkers, few studies systematically revealed the tra...

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Autores principales:	Liyuan Wang, Tianyang Yu, Xiaohui Zhang, Xiaojun Cai, He Sun
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	primary open-angle glaucoma ceRNA transcription factors immune infiltration biomarkers Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/0d1647afe8bb48a186a37845009162c0
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spelling	oai:doaj.org-article:0d1647afe8bb48a186a37845009162c02021-12-03T06:55:56ZNetwork Integration Analysis and Immune Infiltration Analysis Reveal Potential Biomarkers for Primary Open-Angle Glaucoma2296-634X10.3389/fcell.2021.793638https://doaj.org/article/0d1647afe8bb48a186a37845009162c02021-12-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fcell.2021.793638/fullhttps://doaj.org/toc/2296-634XPrimary open-angle glaucoma (POAG) is a progressive optic neuropathy and its damage to vision is irreversible. Therefore, early diagnosis assisted by biomarkers is essential. Although there were multiple researches on the identification of POAG biomarkers, few studies systematically revealed the transcriptome dysregulation mechanism of POAG from the perspective of pre- and post-transcription of genes. Here, we have collected multiple sets of POAG’s aqueous humor (AH) tissue transcription profiles covering long non-coding RNA (lncRNA), mRNA and mircoRNA (miRNA). Through differential expression analysis, we identified thousands of significant differentially expressed genes (DEGs) between the AH tissue of POAG and non-glaucoma. Further, the DEGs were used to construct a competing endogenous RNA (ceRNA) regulatory network and 1,653 qualified lncRNA-miRNA-mRNA regulatory units were identified. Two ceRNA regulatory subnets were identified based on the random walk algorithm and revealed to be involved in the regulation of multiple complex diseases. At the pre-transcriptional regulation level, a transcriptional regulatory network was constructed and three transcription factors (FOS, ATF4, and RELB) were identified to regulate the expression of multiple genes and participate in the regulation of T cells. Moreover, we revealed the immune desert status of AH tissue for POAG patients based on immune infiltration analysis and identified a specific AL590666.2-hsa−miR−339−5p-UROD axis can be used as a biomarker of POAG. Taken together, the identification of regulatory mechanisms and biomarkers will contribute to the individualized diagnosis and treatment for POAG.Liyuan WangTianyang YuXiaohui ZhangXiaojun CaiHe SunFrontiers Media S.A.articleprimary open-angle glaucomaceRNAtranscription factorsimmune infiltrationbiomarkersBiology (General)QH301-705.5ENFrontiers in Cell and Developmental Biology, Vol 9 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	primary open-angle glaucoma ceRNA transcription factors immune infiltration biomarkers Biology (General) QH301-705.5
spellingShingle	primary open-angle glaucoma ceRNA transcription factors immune infiltration biomarkers Biology (General) QH301-705.5 Liyuan Wang Tianyang Yu Xiaohui Zhang Xiaojun Cai He Sun Network Integration Analysis and Immune Infiltration Analysis Reveal Potential Biomarkers for Primary Open-Angle Glaucoma
description	Primary open-angle glaucoma (POAG) is a progressive optic neuropathy and its damage to vision is irreversible. Therefore, early diagnosis assisted by biomarkers is essential. Although there were multiple researches on the identification of POAG biomarkers, few studies systematically revealed the transcriptome dysregulation mechanism of POAG from the perspective of pre- and post-transcription of genes. Here, we have collected multiple sets of POAG’s aqueous humor (AH) tissue transcription profiles covering long non-coding RNA (lncRNA), mRNA and mircoRNA (miRNA). Through differential expression analysis, we identified thousands of significant differentially expressed genes (DEGs) between the AH tissue of POAG and non-glaucoma. Further, the DEGs were used to construct a competing endogenous RNA (ceRNA) regulatory network and 1,653 qualified lncRNA-miRNA-mRNA regulatory units were identified. Two ceRNA regulatory subnets were identified based on the random walk algorithm and revealed to be involved in the regulation of multiple complex diseases. At the pre-transcriptional regulation level, a transcriptional regulatory network was constructed and three transcription factors (FOS, ATF4, and RELB) were identified to regulate the expression of multiple genes and participate in the regulation of T cells. Moreover, we revealed the immune desert status of AH tissue for POAG patients based on immune infiltration analysis and identified a specific AL590666.2-hsa−miR−339−5p-UROD axis can be used as a biomarker of POAG. Taken together, the identification of regulatory mechanisms and biomarkers will contribute to the individualized diagnosis and treatment for POAG.
format	article
author	Liyuan Wang Tianyang Yu Xiaohui Zhang Xiaojun Cai He Sun
author_facet	Liyuan Wang Tianyang Yu Xiaohui Zhang Xiaojun Cai He Sun
author_sort	Liyuan Wang
title	Network Integration Analysis and Immune Infiltration Analysis Reveal Potential Biomarkers for Primary Open-Angle Glaucoma
title_short	Network Integration Analysis and Immune Infiltration Analysis Reveal Potential Biomarkers for Primary Open-Angle Glaucoma
title_full	Network Integration Analysis and Immune Infiltration Analysis Reveal Potential Biomarkers for Primary Open-Angle Glaucoma
title_fullStr	Network Integration Analysis and Immune Infiltration Analysis Reveal Potential Biomarkers for Primary Open-Angle Glaucoma
title_full_unstemmed	Network Integration Analysis and Immune Infiltration Analysis Reveal Potential Biomarkers for Primary Open-Angle Glaucoma
title_sort	network integration analysis and immune infiltration analysis reveal potential biomarkers for primary open-angle glaucoma
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/0d1647afe8bb48a186a37845009162c0
work_keys_str_mv	AT liyuanwang networkintegrationanalysisandimmuneinfiltrationanalysisrevealpotentialbiomarkersforprimaryopenangleglaucoma AT tianyangyu networkintegrationanalysisandimmuneinfiltrationanalysisrevealpotentialbiomarkersforprimaryopenangleglaucoma AT xiaohuizhang networkintegrationanalysisandimmuneinfiltrationanalysisrevealpotentialbiomarkersforprimaryopenangleglaucoma AT xiaojuncai networkintegrationanalysisandimmuneinfiltrationanalysisrevealpotentialbiomarkersforprimaryopenangleglaucoma AT hesun networkintegrationanalysisandimmuneinfiltrationanalysisrevealpotentialbiomarkersforprimaryopenangleglaucoma
_version_	1718373853853384704

Network Integration Analysis and Immune Infiltration Analysis Reveal Potential Biomarkers for Primary Open-Angle Glaucoma

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