Axial stretch regulates rat tail collecting lymphatic vessel contractions

Abstract Lymphatic contractions play a fundamental role in maintaining tissue and organ homeostasis. The lymphatic system relies on orchestrated contraction of collecting lymphatic vessels, via lymphatic muscle cells and one-way valves, to transport lymph from the interstitial space back to the grea...

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Autores principales:	Mohammad S. Razavi, Julie Leonard-Duke, Becky Hardie, J. Brandon Dixon, Rudolph L. Gleason
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2020
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/0d1bac5ff6194994adf895223f743e4d
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spelling	oai:doaj.org-article:0d1bac5ff6194994adf895223f743e4d2021-12-02T18:17:54ZAxial stretch regulates rat tail collecting lymphatic vessel contractions10.1038/s41598-020-62799-x2045-2322https://doaj.org/article/0d1bac5ff6194994adf895223f743e4d2020-04-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-62799-xhttps://doaj.org/toc/2045-2322Abstract Lymphatic contractions play a fundamental role in maintaining tissue and organ homeostasis. The lymphatic system relies on orchestrated contraction of collecting lymphatic vessels, via lymphatic muscle cells and one-way valves, to transport lymph from the interstitial space back to the great veins, against an adverse pressure gradient. Circumferential stretch is known to regulate contractile function in collecting lymphatic vessels; however, less is known about the role of axial stretch in regulating contraction. It is likely that collecting lymphatic vessels are under axial strain in vivo and that the opening and closing of lymphatic valves leads to significant changes in axial strain throughout the pumping cycle. The purpose of this paper is to quantify the responsiveness of lympatic pumping to altered axial stretch. In situ measurements suggest that rat tail collecting lymphatic vessels are under an axial stretch of ~1.24 under normal physiological loads. Ex vivo experiments on isolated rat tail collecting lymphatics showed that the contractile metrics such as contractile amplitude, frequency, ejection fraction, and fractional pump flow are sensitive to axial stretch. Multiphoton microscopy showed that the predominant orientation of collagen fibers is in the axial direction, while lymphatic muscle cell nuclei and actin fibers are oriented in both circumferential and longitudinal directions, suggesting an axial component to contraction. Taken together, these results demonstrate the significance of axial stretch in lymphatic contractile function, suggest that axial stretch may play an important role in regulating lymph transport, and demonstrate that changes in axial strains could be an important factor in disease progression.Mohammad S. RazaviJulie Leonard-DukeBecky HardieJ. Brandon DixonRudolph L. GleasonNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 10, Iss 1, Pp 1-11 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Mohammad S. Razavi Julie Leonard-Duke Becky Hardie J. Brandon Dixon Rudolph L. Gleason Axial stretch regulates rat tail collecting lymphatic vessel contractions
description	Abstract Lymphatic contractions play a fundamental role in maintaining tissue and organ homeostasis. The lymphatic system relies on orchestrated contraction of collecting lymphatic vessels, via lymphatic muscle cells and one-way valves, to transport lymph from the interstitial space back to the great veins, against an adverse pressure gradient. Circumferential stretch is known to regulate contractile function in collecting lymphatic vessels; however, less is known about the role of axial stretch in regulating contraction. It is likely that collecting lymphatic vessels are under axial strain in vivo and that the opening and closing of lymphatic valves leads to significant changes in axial strain throughout the pumping cycle. The purpose of this paper is to quantify the responsiveness of lympatic pumping to altered axial stretch. In situ measurements suggest that rat tail collecting lymphatic vessels are under an axial stretch of ~1.24 under normal physiological loads. Ex vivo experiments on isolated rat tail collecting lymphatics showed that the contractile metrics such as contractile amplitude, frequency, ejection fraction, and fractional pump flow are sensitive to axial stretch. Multiphoton microscopy showed that the predominant orientation of collagen fibers is in the axial direction, while lymphatic muscle cell nuclei and actin fibers are oriented in both circumferential and longitudinal directions, suggesting an axial component to contraction. Taken together, these results demonstrate the significance of axial stretch in lymphatic contractile function, suggest that axial stretch may play an important role in regulating lymph transport, and demonstrate that changes in axial strains could be an important factor in disease progression.
format	article
author	Mohammad S. Razavi Julie Leonard-Duke Becky Hardie J. Brandon Dixon Rudolph L. Gleason
author_facet	Mohammad S. Razavi Julie Leonard-Duke Becky Hardie J. Brandon Dixon Rudolph L. Gleason
author_sort	Mohammad S. Razavi
title	Axial stretch regulates rat tail collecting lymphatic vessel contractions
title_short	Axial stretch regulates rat tail collecting lymphatic vessel contractions
title_full	Axial stretch regulates rat tail collecting lymphatic vessel contractions
title_fullStr	Axial stretch regulates rat tail collecting lymphatic vessel contractions
title_full_unstemmed	Axial stretch regulates rat tail collecting lymphatic vessel contractions
title_sort	axial stretch regulates rat tail collecting lymphatic vessel contractions
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/0d1bac5ff6194994adf895223f743e4d
work_keys_str_mv	AT mohammadsrazavi axialstretchregulatesrattailcollectinglymphaticvesselcontractions AT julieleonardduke axialstretchregulatesrattailcollectinglymphaticvesselcontractions AT beckyhardie axialstretchregulatesrattailcollectinglymphaticvesselcontractions AT jbrandondixon axialstretchregulatesrattailcollectinglymphaticvesselcontractions AT rudolphlgleason axialstretchregulatesrattailcollectinglymphaticvesselcontractions
_version_	1718378256941449216

Axial stretch regulates rat tail collecting lymphatic vessel contractions

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