Optimizing the synthesis and purification of MS2 virus like particles

Abstract Introducing bacteriophage MS2 virus-like particles (VLPs) as gene and drug delivery tools increases the demand for optimizing their production and purification procedure. PEG precipitation method is used efficiently to purify VLPs, while the effects of pH and different electrolytes on the s...

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Autores principales:	Khadijeh Hashemi, Mohammad Mahdi Ghahramani Seno, Mohammad Reza Ahmadian, Bizhan Malaekeh-Nikouei, Mohammad Reza Bassami, Hesam Dehghani, Amir Afkhami-Goli
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/0d1c8ffd1d7f4708bef03beb07f183f2
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spelling	oai:doaj.org-article:0d1c8ffd1d7f4708bef03beb07f183f22021-12-02T18:37:11ZOptimizing the synthesis and purification of MS2 virus like particles10.1038/s41598-021-98706-12045-2322https://doaj.org/article/0d1c8ffd1d7f4708bef03beb07f183f22021-10-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-98706-1https://doaj.org/toc/2045-2322Abstract Introducing bacteriophage MS2 virus-like particles (VLPs) as gene and drug delivery tools increases the demand for optimizing their production and purification procedure. PEG precipitation method is used efficiently to purify VLPs, while the effects of pH and different electrolytes on the stability, size, and homogeneity of purified MS2 VLPs, and the encapsulated RNA sequences remained to be elucidated. In this regard, a vector, capable of producing VLP with an shRNA packed inside was prepared. The resulting VLPs in different buffers/solutions were assessed for their size, polydispersity index, and ability to protect the enclosed shRNA. We report that among Tris, HEPES, and PBS, with or without NaNO3, and also NaNO3 alone in different pH and ionic concentrations, the 100 mM NaNO3-Tris buffer with pH:8 can be used as a new and optimal MS2 VLP production buffer, capable of inhibiting the VLPs aggregation. These VLPs show a size range of 27-30 nm and suitable homogeneity with minimum 12-month stability at 4 °C. Moreover, the resulting MS2 VLPs were highly efficient and stable for at least 48 h in conditions similar to in vivo. These features of MS2 VLPs produced in the newly introduced buffer make them an appropriate candidate for therapeutic agents’ delivery.Khadijeh HashemiMohammad Mahdi Ghahramani SenoMohammad Reza AhmadianBizhan Malaekeh-NikoueiMohammad Reza BassamiHesam DehghaniAmir Afkhami-GoliNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-14 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Khadijeh Hashemi Mohammad Mahdi Ghahramani Seno Mohammad Reza Ahmadian Bizhan Malaekeh-Nikouei Mohammad Reza Bassami Hesam Dehghani Amir Afkhami-Goli Optimizing the synthesis and purification of MS2 virus like particles
description	Abstract Introducing bacteriophage MS2 virus-like particles (VLPs) as gene and drug delivery tools increases the demand for optimizing their production and purification procedure. PEG precipitation method is used efficiently to purify VLPs, while the effects of pH and different electrolytes on the stability, size, and homogeneity of purified MS2 VLPs, and the encapsulated RNA sequences remained to be elucidated. In this regard, a vector, capable of producing VLP with an shRNA packed inside was prepared. The resulting VLPs in different buffers/solutions were assessed for their size, polydispersity index, and ability to protect the enclosed shRNA. We report that among Tris, HEPES, and PBS, with or without NaNO3, and also NaNO3 alone in different pH and ionic concentrations, the 100 mM NaNO3-Tris buffer with pH:8 can be used as a new and optimal MS2 VLP production buffer, capable of inhibiting the VLPs aggregation. These VLPs show a size range of 27-30 nm and suitable homogeneity with minimum 12-month stability at 4 °C. Moreover, the resulting MS2 VLPs were highly efficient and stable for at least 48 h in conditions similar to in vivo. These features of MS2 VLPs produced in the newly introduced buffer make them an appropriate candidate for therapeutic agents’ delivery.
format	article
author	Khadijeh Hashemi Mohammad Mahdi Ghahramani Seno Mohammad Reza Ahmadian Bizhan Malaekeh-Nikouei Mohammad Reza Bassami Hesam Dehghani Amir Afkhami-Goli
author_facet	Khadijeh Hashemi Mohammad Mahdi Ghahramani Seno Mohammad Reza Ahmadian Bizhan Malaekeh-Nikouei Mohammad Reza Bassami Hesam Dehghani Amir Afkhami-Goli
author_sort	Khadijeh Hashemi
title	Optimizing the synthesis and purification of MS2 virus like particles
title_short	Optimizing the synthesis and purification of MS2 virus like particles
title_full	Optimizing the synthesis and purification of MS2 virus like particles
title_fullStr	Optimizing the synthesis and purification of MS2 virus like particles
title_full_unstemmed	Optimizing the synthesis and purification of MS2 virus like particles
title_sort	optimizing the synthesis and purification of ms2 virus like particles
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/0d1c8ffd1d7f4708bef03beb07f183f2
work_keys_str_mv	AT khadijehhashemi optimizingthesynthesisandpurificationofms2viruslikeparticles AT mohammadmahdighahramaniseno optimizingthesynthesisandpurificationofms2viruslikeparticles AT mohammadrezaahmadian optimizingthesynthesisandpurificationofms2viruslikeparticles AT bizhanmalaekehnikouei optimizingthesynthesisandpurificationofms2viruslikeparticles AT mohammadrezabassami optimizingthesynthesisandpurificationofms2viruslikeparticles AT hesamdehghani optimizingthesynthesisandpurificationofms2viruslikeparticles AT amirafkhamigoli optimizingthesynthesisandpurificationofms2viruslikeparticles
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Optimizing the synthesis and purification of MS2 virus like particles

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