HOXA7, HOXA9, and HOXA10 are differentially expressed in clival and sacral chordomas

Abstract Chordomas are rare tumours of the bone arising along the spine from clivus to sacrum. We compared three chordoma cell lines of the clivus region including the newly established clivus chordoma cell line, U-CH14, with nine chordoma cell lines originating from sacral primaries by morphology,...

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Autores principales:	Daniela Jäger, Thomas F. E. Barth, Silke Brüderlein, Angelika Scheuerle, Beate Rinner, Adrian von Witzleben, André Lechel, Patrick Meyer, Regine Mayer-Steinacker, Alexandra von Baer, Markus Schultheiss, Christian R. Wirtz, Peter Möller, Kevin Mellert
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/0d1eed0325ea49689979e4d5afd31ace
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spelling	oai:doaj.org-article:0d1eed0325ea49689979e4d5afd31ace2021-12-02T12:30:17ZHOXA7, HOXA9, and HOXA10 are differentially expressed in clival and sacral chordomas10.1038/s41598-017-02174-52045-2322https://doaj.org/article/0d1eed0325ea49689979e4d5afd31ace2017-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-02174-5https://doaj.org/toc/2045-2322Abstract Chordomas are rare tumours of the bone arising along the spine from clivus to sacrum. We compared three chordoma cell lines of the clivus region including the newly established clivus chordoma cell line, U-CH14, with nine chordoma cell lines originating from sacral primaries by morphology, on genomic and expression levels and with patient samples from our chordoma tissue bank. Clinically, chordomas of the clivus were generally smaller in size at presentation and patients with sacral chordomas had more metastases and more often recurrent disease. All chordoma cell lines had a typical physaliphorous morphology and expressed brachyury, S100-protein and cytokeratin. By expression analyses we detected differentially expressed genes in the clivus derived cell lines as compared to the sacral cell lines. Among these were HOXA7, HOXA9, and HOXA10 known to be important for the development of the anterior-posterior body axis. These results were confirmed by qPCR. Immunohistologically, clivus chordomas had no or very low levels of HOXA10 protein while sacral chordomas showed a strong nuclear positivity in all samples analysed. This differential expression of HOX genes in chordomas of the clivus and sacrum suggests an oncofetal mechanism in gene regulation linked to the anatomic site.Daniela JägerThomas F. E. BarthSilke BrüderleinAngelika ScheuerleBeate RinnerAdrian von WitzlebenAndré LechelPatrick MeyerRegine Mayer-SteinackerAlexandra von BaerMarkus SchultheissChristian R. WirtzPeter MöllerKevin MellertNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-12 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Daniela Jäger Thomas F. E. Barth Silke Brüderlein Angelika Scheuerle Beate Rinner Adrian von Witzleben André Lechel Patrick Meyer Regine Mayer-Steinacker Alexandra von Baer Markus Schultheiss Christian R. Wirtz Peter Möller Kevin Mellert HOXA7, HOXA9, and HOXA10 are differentially expressed in clival and sacral chordomas
description	Abstract Chordomas are rare tumours of the bone arising along the spine from clivus to sacrum. We compared three chordoma cell lines of the clivus region including the newly established clivus chordoma cell line, U-CH14, with nine chordoma cell lines originating from sacral primaries by morphology, on genomic and expression levels and with patient samples from our chordoma tissue bank. Clinically, chordomas of the clivus were generally smaller in size at presentation and patients with sacral chordomas had more metastases and more often recurrent disease. All chordoma cell lines had a typical physaliphorous morphology and expressed brachyury, S100-protein and cytokeratin. By expression analyses we detected differentially expressed genes in the clivus derived cell lines as compared to the sacral cell lines. Among these were HOXA7, HOXA9, and HOXA10 known to be important for the development of the anterior-posterior body axis. These results were confirmed by qPCR. Immunohistologically, clivus chordomas had no or very low levels of HOXA10 protein while sacral chordomas showed a strong nuclear positivity in all samples analysed. This differential expression of HOX genes in chordomas of the clivus and sacrum suggests an oncofetal mechanism in gene regulation linked to the anatomic site.
format	article
author	Daniela Jäger Thomas F. E. Barth Silke Brüderlein Angelika Scheuerle Beate Rinner Adrian von Witzleben André Lechel Patrick Meyer Regine Mayer-Steinacker Alexandra von Baer Markus Schultheiss Christian R. Wirtz Peter Möller Kevin Mellert
author_facet	Daniela Jäger Thomas F. E. Barth Silke Brüderlein Angelika Scheuerle Beate Rinner Adrian von Witzleben André Lechel Patrick Meyer Regine Mayer-Steinacker Alexandra von Baer Markus Schultheiss Christian R. Wirtz Peter Möller Kevin Mellert
author_sort	Daniela Jäger
title	HOXA7, HOXA9, and HOXA10 are differentially expressed in clival and sacral chordomas
title_short	HOXA7, HOXA9, and HOXA10 are differentially expressed in clival and sacral chordomas
title_full	HOXA7, HOXA9, and HOXA10 are differentially expressed in clival and sacral chordomas
title_fullStr	HOXA7, HOXA9, and HOXA10 are differentially expressed in clival and sacral chordomas
title_full_unstemmed	HOXA7, HOXA9, and HOXA10 are differentially expressed in clival and sacral chordomas
title_sort	hoxa7, hoxa9, and hoxa10 are differentially expressed in clival and sacral chordomas
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/0d1eed0325ea49689979e4d5afd31ace
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HOXA7, HOXA9, and HOXA10 are differentially expressed in clival and sacral chordomas

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