Diazotrophic Behaviour in a Non-Sterile Bioreactor: The Effect of O2-Availability

The behaviour of a locally isolated diazotrophic consortium was investigated with the prospect of agricultural applications. A repeatable culture was obtained in a non-sterile bioreactor. Metagenomic analysis indicated Chryseobacterium ssp. and Flavobacterium ss...

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Autores principales:	Amber Yasemin Shirin de Zoete, Hendrik Gideon Brink, Joshua Cornelus Beukes, Ignatius Leopoldus van Rooyen, Willie Nicol
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	diazotrophs bio-fertilizer nitrogen fixation ATP down-regulation Chemical technology TP1-1185 Chemistry QD1-999
Acceso en línea:	https://doaj.org/article/0d22789daa3e4079802622f8a2c1c432
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spelling	oai:doaj.org-article:0d22789daa3e4079802622f8a2c1c4322021-11-25T18:51:41ZDiazotrophic Behaviour in a Non-Sterile Bioreactor: The Effect of O<sub>2</sub>-Availability10.3390/pr91120392227-9717https://doaj.org/article/0d22789daa3e4079802622f8a2c1c4322021-11-01T00:00:00Zhttps://www.mdpi.com/2227-9717/9/11/2039https://doaj.org/toc/2227-9717The behaviour of a locally isolated diazotrophic consortium was investigated with the prospect of agricultural applications. A repeatable culture was obtained in a non-sterile bioreactor. Metagenomic analysis indicated <i>Chryseobacterium</i> ssp. and <i>Flavobacterium</i> ssp. were the dominant species, making up approximately 50% of the microbial community. The oxygen supply was varied and mass-transfer limited growth was attained under all experimental conditions. Negligible amounts of aqueous metabolites were formed, indicating a high selectivity towards biomass production. High oxygen availability resulted in decreased growth efficiencies i.e., the specific energy requirements for biomass synthesis. This was attributed to reduced electron transport chain efficiencies and nitrogenase protection mechanisms. Mass and energy balances indicated that sessile biomass with a high C:N served as a carbon sink. The most efficient growth was measured at an aeration feed composition of 21% oxygen and 79% nitrogen. The study presents one of the only known investigations of operational conditions on diazotrophic growth in a non-sterile bioreactor. In addition, it provides a strong foundation for the development of a Biological Nitrogen Fixation process with scaling potential.Amber Yasemin Shirin de ZoeteHendrik Gideon BrinkJoshua Cornelus BeukesIgnatius Leopoldus van RooyenWillie NicolMDPI AGarticlediazotrophsbio-fertilizernitrogen fixationATP down-regulationChemical technologyTP1-1185ChemistryQD1-999ENProcesses, Vol 9, Iss 2039, p 2039 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	diazotrophs bio-fertilizer nitrogen fixation ATP down-regulation Chemical technology TP1-1185 Chemistry QD1-999
spellingShingle	diazotrophs bio-fertilizer nitrogen fixation ATP down-regulation Chemical technology TP1-1185 Chemistry QD1-999 Amber Yasemin Shirin de Zoete Hendrik Gideon Brink Joshua Cornelus Beukes Ignatius Leopoldus van Rooyen Willie Nicol Diazotrophic Behaviour in a Non-Sterile Bioreactor: The Effect of O<sub>2</sub>-Availability
description	The behaviour of a locally isolated diazotrophic consortium was investigated with the prospect of agricultural applications. A repeatable culture was obtained in a non-sterile bioreactor. Metagenomic analysis indicated <i>Chryseobacterium</i> ssp. and <i>Flavobacterium</i> ssp. were the dominant species, making up approximately 50% of the microbial community. The oxygen supply was varied and mass-transfer limited growth was attained under all experimental conditions. Negligible amounts of aqueous metabolites were formed, indicating a high selectivity towards biomass production. High oxygen availability resulted in decreased growth efficiencies i.e., the specific energy requirements for biomass synthesis. This was attributed to reduced electron transport chain efficiencies and nitrogenase protection mechanisms. Mass and energy balances indicated that sessile biomass with a high C:N served as a carbon sink. The most efficient growth was measured at an aeration feed composition of 21% oxygen and 79% nitrogen. The study presents one of the only known investigations of operational conditions on diazotrophic growth in a non-sterile bioreactor. In addition, it provides a strong foundation for the development of a Biological Nitrogen Fixation process with scaling potential.
format	article
author	Amber Yasemin Shirin de Zoete Hendrik Gideon Brink Joshua Cornelus Beukes Ignatius Leopoldus van Rooyen Willie Nicol
author_facet	Amber Yasemin Shirin de Zoete Hendrik Gideon Brink Joshua Cornelus Beukes Ignatius Leopoldus van Rooyen Willie Nicol
author_sort	Amber Yasemin Shirin de Zoete
title	Diazotrophic Behaviour in a Non-Sterile Bioreactor: The Effect of O<sub>2</sub>-Availability
title_short	Diazotrophic Behaviour in a Non-Sterile Bioreactor: The Effect of O<sub>2</sub>-Availability
title_full	Diazotrophic Behaviour in a Non-Sterile Bioreactor: The Effect of O<sub>2</sub>-Availability
title_fullStr	Diazotrophic Behaviour in a Non-Sterile Bioreactor: The Effect of O<sub>2</sub>-Availability
title_full_unstemmed	Diazotrophic Behaviour in a Non-Sterile Bioreactor: The Effect of O<sub>2</sub>-Availability
title_sort	diazotrophic behaviour in a non-sterile bioreactor: the effect of o<sub>2</sub>-availability
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/0d22789daa3e4079802622f8a2c1c432
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Diazotrophic Behaviour in a Non-Sterile Bioreactor: The Effect of O<sub>2</sub>-Availability

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