Strong association of 677 C>T substitution in the MTHFR gene with male infertility--a study on an indian population and a meta-analysis.

<h4>Background</h4>Methylenetetrahydrofolate reductase (MTHFR) is an important enzyme of folate and methionine metabolism, making it crucial for DNA synthesis and methylation. The objective of this study was to analyze MTHFR gene 677C>T polymorphism in infertile male individuals from...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Nishi Gupta, Saraswati Gupta, Madhukar Dama, Archana David, Geeta Khanna, Anil Khanna, Singh Rajender
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2011
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/0d238e99e9b94d7399669181a32812bc
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:0d238e99e9b94d7399669181a32812bc
record_format	dspace
spelling	oai:doaj.org-article:0d238e99e9b94d7399669181a32812bc2021-11-18T06:49:52ZStrong association of 677 C>T substitution in the MTHFR gene with male infertility--a study on an indian population and a meta-analysis.1932-620310.1371/journal.pone.0022277https://doaj.org/article/0d238e99e9b94d7399669181a32812bc2011-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/21799811/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background</h4>Methylenetetrahydrofolate reductase (MTHFR) is an important enzyme of folate and methionine metabolism, making it crucial for DNA synthesis and methylation. The objective of this study was to analyze MTHFR gene 677C>T polymorphism in infertile male individuals from North India, followed by a meta-analysis on our data and published studies.<h4>Methodology/principal findings</h4>We undertook genotyping on a total of 837 individuals including well characterized infertile (N = 522) and confirmed fertile (N = 315) individuals. The SNP was typed by direct DNA sequencing. Chi square test was done for statistical analysis. Published studies were searched using appropriate keywords. Source of data collection for meta-analysis included 'Pubmed', 'Ovid' and 'Google Scholar'. Those studies analyzing 677C>T polymorphism in male infertility and presenting all relevant data were included in meta-analysis. The genotype data for infertile subjects and fertile controls was extracted from each study. Chi square test was done to obtain odds ratio (OR) and p-value. Meta-analysis was performed using Comprehensive Meta-analysis software (Version 2). The frequency of mutant (T) allele (p = 0.0025) and genotypes (CT+TT) (p = 0.0187) was significantly higher in infertile individuals in comparison to fertile controls in our case-control study. The overall summary estimate (OR) for allele and genotype meta-analysis were 1.304 (p = 0.000), 1.310 (p = 0.000), respectively, establishing significant association of 677C>T polymorphism with male infertility.<h4>Conclusions/significance</h4>677C>T substitution associated strongly with male infertility in Indian population. Allele and genotype meta-analysis also supported its strong correlation with male infertility, thus establishing it as a risk factor.Nishi GuptaSaraswati GuptaMadhukar DamaArchana DavidGeeta KhannaAnil KhannaSingh RajenderPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 6, Iss 7, p e22277 (2011)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Nishi Gupta Saraswati Gupta Madhukar Dama Archana David Geeta Khanna Anil Khanna Singh Rajender Strong association of 677 C>T substitution in the MTHFR gene with male infertility--a study on an indian population and a meta-analysis.
description	<h4>Background</h4>Methylenetetrahydrofolate reductase (MTHFR) is an important enzyme of folate and methionine metabolism, making it crucial for DNA synthesis and methylation. The objective of this study was to analyze MTHFR gene 677C>T polymorphism in infertile male individuals from North India, followed by a meta-analysis on our data and published studies.<h4>Methodology/principal findings</h4>We undertook genotyping on a total of 837 individuals including well characterized infertile (N = 522) and confirmed fertile (N = 315) individuals. The SNP was typed by direct DNA sequencing. Chi square test was done for statistical analysis. Published studies were searched using appropriate keywords. Source of data collection for meta-analysis included 'Pubmed', 'Ovid' and 'Google Scholar'. Those studies analyzing 677C>T polymorphism in male infertility and presenting all relevant data were included in meta-analysis. The genotype data for infertile subjects and fertile controls was extracted from each study. Chi square test was done to obtain odds ratio (OR) and p-value. Meta-analysis was performed using Comprehensive Meta-analysis software (Version 2). The frequency of mutant (T) allele (p = 0.0025) and genotypes (CT+TT) (p = 0.0187) was significantly higher in infertile individuals in comparison to fertile controls in our case-control study. The overall summary estimate (OR) for allele and genotype meta-analysis were 1.304 (p = 0.000), 1.310 (p = 0.000), respectively, establishing significant association of 677C>T polymorphism with male infertility.<h4>Conclusions/significance</h4>677C>T substitution associated strongly with male infertility in Indian population. Allele and genotype meta-analysis also supported its strong correlation with male infertility, thus establishing it as a risk factor.
format	article
author	Nishi Gupta Saraswati Gupta Madhukar Dama Archana David Geeta Khanna Anil Khanna Singh Rajender
author_facet	Nishi Gupta Saraswati Gupta Madhukar Dama Archana David Geeta Khanna Anil Khanna Singh Rajender
author_sort	Nishi Gupta
title	Strong association of 677 C>T substitution in the MTHFR gene with male infertility--a study on an indian population and a meta-analysis.
title_short	Strong association of 677 C>T substitution in the MTHFR gene with male infertility--a study on an indian population and a meta-analysis.
title_full	Strong association of 677 C>T substitution in the MTHFR gene with male infertility--a study on an indian population and a meta-analysis.
title_fullStr	Strong association of 677 C>T substitution in the MTHFR gene with male infertility--a study on an indian population and a meta-analysis.
title_full_unstemmed	Strong association of 677 C>T substitution in the MTHFR gene with male infertility--a study on an indian population and a meta-analysis.
title_sort	strong association of 677 c>t substitution in the mthfr gene with male infertility--a study on an indian population and a meta-analysis.
publisher	Public Library of Science (PLoS)
publishDate	2011
url	https://doaj.org/article/0d238e99e9b94d7399669181a32812bc
work_keys_str_mv	AT nishigupta strongassociationof677ctsubstitutioninthemthfrgenewithmaleinfertilityastudyonanindianpopulationandametaanalysis AT saraswatigupta strongassociationof677ctsubstitutioninthemthfrgenewithmaleinfertilityastudyonanindianpopulationandametaanalysis AT madhukardama strongassociationof677ctsubstitutioninthemthfrgenewithmaleinfertilityastudyonanindianpopulationandametaanalysis AT archanadavid strongassociationof677ctsubstitutioninthemthfrgenewithmaleinfertilityastudyonanindianpopulationandametaanalysis AT geetakhanna strongassociationof677ctsubstitutioninthemthfrgenewithmaleinfertilityastudyonanindianpopulationandametaanalysis AT anilkhanna strongassociationof677ctsubstitutioninthemthfrgenewithmaleinfertilityastudyonanindianpopulationandametaanalysis AT singhrajender strongassociationof677ctsubstitutioninthemthfrgenewithmaleinfertilityastudyonanindianpopulationandametaanalysis
_version_	1718424333835042816

Strong association of 677 C>T substitution in the MTHFR gene with male infertility--a study on an indian population and a meta-analysis.

Ejemplares similares