Exosomes-mediated phenotypic switch of macrophages in the immune microenvironment after spinal cord injury

Although accumulating evidence indicated that modulating macrophage polarization could ameliorate the immune microenvironment and facilitate the repair of spinal cord injury (SCI), the underlying mechanism of macrophage phenotypic switch is still poorly understood. Exosomes (Exos), a potential tool...

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Autores principales:	Peng Peng, Hao Yu, Cong Xing, Bo Tao, Chao Li, Jingyuan Huang, Guangzhi Ning, Bin Zhang, Shiqing Feng
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2021
Materias:	Macrophage polarization Spinal cord injury Exosomes MiRNA-mRNA network Immune microenvironment Therapeutics. Pharmacology RM1-950
Acceso en línea:	https://doaj.org/article/0d2984b1cb53407da935e0bf812b7fb7
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spelling	oai:doaj.org-article:0d2984b1cb53407da935e0bf812b7fb72021-11-14T04:29:31ZExosomes-mediated phenotypic switch of macrophages in the immune microenvironment after spinal cord injury0753-332210.1016/j.biopha.2021.112311https://doaj.org/article/0d2984b1cb53407da935e0bf812b7fb72021-12-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S0753332221010957https://doaj.org/toc/0753-3322Although accumulating evidence indicated that modulating macrophage polarization could ameliorate the immune microenvironment and facilitate the repair of spinal cord injury (SCI), the underlying mechanism of macrophage phenotypic switch is still poorly understood. Exosomes (Exos), a potential tool of cell-to-cell communication, may play important roles in cell reprogramming. Herein, we investigated the roles of macrophages-derived exosomes played for macrophage polarization in the SCI immune microenvironment. In this study, we found the fraction of M2 macrophages was markedly decreased after SCI. Moreover, the M2 macrophages-derived exosomes could increase the percentage of M2 macrophages, decrease that of M1 macrophages while the M1 macrophages-derived exosomes acted oppositely. According to the results of in silico analyses and molecular experiments verification, this phenotypic switch might be mediated by the exosomal miRNA-mRNA network, in which the miR-23a-3p/PTEN/PI3K/AKT axis might play an important role. In conclusion, our study suggests macrophage polarization that regulated by various interventions might be mediated by their own exosomes at last. Moreover, M2 macrophages-derived exosomes could promote M2 macrophage polarization via the potential miRNA-mRNA network. Considering its potential of modulating polarization, M2 macrophages-derived exosomes may be a promising therapeutic agent for SCI repair.Peng PengHao YuCong XingBo TaoChao LiJingyuan HuangGuangzhi NingBin ZhangShiqing FengElsevierarticleMacrophage polarizationSpinal cord injuryExosomesMiRNA-mRNA networkImmune microenvironmentTherapeutics. PharmacologyRM1-950ENBiomedicine & Pharmacotherapy, Vol 144, Iss , Pp 112311- (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Macrophage polarization Spinal cord injury Exosomes MiRNA-mRNA network Immune microenvironment Therapeutics. Pharmacology RM1-950
spellingShingle	Macrophage polarization Spinal cord injury Exosomes MiRNA-mRNA network Immune microenvironment Therapeutics. Pharmacology RM1-950 Peng Peng Hao Yu Cong Xing Bo Tao Chao Li Jingyuan Huang Guangzhi Ning Bin Zhang Shiqing Feng Exosomes-mediated phenotypic switch of macrophages in the immune microenvironment after spinal cord injury
description	Although accumulating evidence indicated that modulating macrophage polarization could ameliorate the immune microenvironment and facilitate the repair of spinal cord injury (SCI), the underlying mechanism of macrophage phenotypic switch is still poorly understood. Exosomes (Exos), a potential tool of cell-to-cell communication, may play important roles in cell reprogramming. Herein, we investigated the roles of macrophages-derived exosomes played for macrophage polarization in the SCI immune microenvironment. In this study, we found the fraction of M2 macrophages was markedly decreased after SCI. Moreover, the M2 macrophages-derived exosomes could increase the percentage of M2 macrophages, decrease that of M1 macrophages while the M1 macrophages-derived exosomes acted oppositely. According to the results of in silico analyses and molecular experiments verification, this phenotypic switch might be mediated by the exosomal miRNA-mRNA network, in which the miR-23a-3p/PTEN/PI3K/AKT axis might play an important role. In conclusion, our study suggests macrophage polarization that regulated by various interventions might be mediated by their own exosomes at last. Moreover, M2 macrophages-derived exosomes could promote M2 macrophage polarization via the potential miRNA-mRNA network. Considering its potential of modulating polarization, M2 macrophages-derived exosomes may be a promising therapeutic agent for SCI repair.
format	article
author	Peng Peng Hao Yu Cong Xing Bo Tao Chao Li Jingyuan Huang Guangzhi Ning Bin Zhang Shiqing Feng
author_facet	Peng Peng Hao Yu Cong Xing Bo Tao Chao Li Jingyuan Huang Guangzhi Ning Bin Zhang Shiqing Feng
author_sort	Peng Peng
title	Exosomes-mediated phenotypic switch of macrophages in the immune microenvironment after spinal cord injury
title_short	Exosomes-mediated phenotypic switch of macrophages in the immune microenvironment after spinal cord injury
title_full	Exosomes-mediated phenotypic switch of macrophages in the immune microenvironment after spinal cord injury
title_fullStr	Exosomes-mediated phenotypic switch of macrophages in the immune microenvironment after spinal cord injury
title_full_unstemmed	Exosomes-mediated phenotypic switch of macrophages in the immune microenvironment after spinal cord injury
title_sort	exosomes-mediated phenotypic switch of macrophages in the immune microenvironment after spinal cord injury
publisher	Elsevier
publishDate	2021
url	https://doaj.org/article/0d2984b1cb53407da935e0bf812b7fb7
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Exosomes-mediated phenotypic switch of macrophages in the immune microenvironment after spinal cord injury

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