Isolation and enrichment of mouse splenic T cells for ex vivo and in vivo T cell receptor stimulation assays

Summary: Specific antigen recognition by T cell receptor (TCR) activates TCR signaling pathway, leading to T cell proliferation and differentiation into effector and memory cells. Herein, we describe protocols for TCR stimulation assays, including procedures for the isolation and enrichment of mouse...

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Autores principales:	Clémence Grosjean, Julie Quessada, Mathis Nozais, Marie Loosveld, Dominique Payet-Bornet, Cyrille Mionnet
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2021
Materias:	Cell Biology Cell culture Cell isolation Flow Cytometry/Mass Cytometry Cell-based Assays Immunology Science (General) Q1-390
Acceso en línea:	https://doaj.org/article/0d2ce91870624e8f9e0a3dcd01ab8efd
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spelling	oai:doaj.org-article:0d2ce91870624e8f9e0a3dcd01ab8efd2021-11-18T04:51:44ZIsolation and enrichment of mouse splenic T cells for ex vivo and in vivo T cell receptor stimulation assays2666-166710.1016/j.xpro.2021.100961https://doaj.org/article/0d2ce91870624e8f9e0a3dcd01ab8efd2021-12-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S2666166721006675https://doaj.org/toc/2666-1667Summary: Specific antigen recognition by T cell receptor (TCR) activates TCR signaling pathway, leading to T cell proliferation and differentiation into effector and memory cells. Herein, we describe protocols for TCR stimulation assays, including procedures for the isolation and enrichment of mouse splenic T cells for ex vivo TCR stimulation with anti-CD3/CD28 antibodies, and the use of ovalbumin-OT-II mouse model for in vivo TCR stimulation. We applied this protocol to show that MYC protein is essential for T cell proliferation and differentiation.For complete details on the use and execution of this protocol, please refer to Nozais et al. (2021).Clémence GrosjeanJulie QuessadaMathis NozaisMarie LoosveldDominique Payet-BornetCyrille MionnetElsevierarticleCell BiologyCell cultureCell isolationFlow Cytometry/Mass CytometryCell-based AssaysImmunologyScience (General)Q1-390ENSTAR Protocols, Vol 2, Iss 4, Pp 100961- (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Cell Biology Cell culture Cell isolation Flow Cytometry/Mass Cytometry Cell-based Assays Immunology Science (General) Q1-390
spellingShingle	Cell Biology Cell culture Cell isolation Flow Cytometry/Mass Cytometry Cell-based Assays Immunology Science (General) Q1-390 Clémence Grosjean Julie Quessada Mathis Nozais Marie Loosveld Dominique Payet-Bornet Cyrille Mionnet Isolation and enrichment of mouse splenic T cells for ex vivo and in vivo T cell receptor stimulation assays
description	Summary: Specific antigen recognition by T cell receptor (TCR) activates TCR signaling pathway, leading to T cell proliferation and differentiation into effector and memory cells. Herein, we describe protocols for TCR stimulation assays, including procedures for the isolation and enrichment of mouse splenic T cells for ex vivo TCR stimulation with anti-CD3/CD28 antibodies, and the use of ovalbumin-OT-II mouse model for in vivo TCR stimulation. We applied this protocol to show that MYC protein is essential for T cell proliferation and differentiation.For complete details on the use and execution of this protocol, please refer to Nozais et al. (2021).
format	article
author	Clémence Grosjean Julie Quessada Mathis Nozais Marie Loosveld Dominique Payet-Bornet Cyrille Mionnet
author_facet	Clémence Grosjean Julie Quessada Mathis Nozais Marie Loosveld Dominique Payet-Bornet Cyrille Mionnet
author_sort	Clémence Grosjean
title	Isolation and enrichment of mouse splenic T cells for ex vivo and in vivo T cell receptor stimulation assays
title_short	Isolation and enrichment of mouse splenic T cells for ex vivo and in vivo T cell receptor stimulation assays
title_full	Isolation and enrichment of mouse splenic T cells for ex vivo and in vivo T cell receptor stimulation assays
title_fullStr	Isolation and enrichment of mouse splenic T cells for ex vivo and in vivo T cell receptor stimulation assays
title_full_unstemmed	Isolation and enrichment of mouse splenic T cells for ex vivo and in vivo T cell receptor stimulation assays
title_sort	isolation and enrichment of mouse splenic t cells for ex vivo and in vivo t cell receptor stimulation assays
publisher	Elsevier
publishDate	2021
url	https://doaj.org/article/0d2ce91870624e8f9e0a3dcd01ab8efd
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Isolation and enrichment of mouse splenic T cells for ex vivo and in vivo T cell receptor stimulation assays

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