Multiple mechanisms contributing to ciprofloxacin resistance among Gram negative bacteria causing infections to cancer patients

Abstract Fluoroquinolones have been used for prophylaxis against infections in cancer patients but their impact on the resistance mechanisms still require further investigation. To elucidate mechanisms underlying ciprofloxacin (CIP) resistance in Gram-negative pathogens causing infections to cancer...

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Autores principales:	Samira M. Hamed, Walid F. Elkhatib, Hadir A. El-Mahallawy, Mai M. Helmy, Mohamed S. Ashour, Khaled M. A. Aboshanab
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/0d34a39e48fa4b6a9a9ac10e7aa954d6
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spelling	oai:doaj.org-article:0d34a39e48fa4b6a9a9ac10e7aa954d62021-12-02T15:07:49ZMultiple mechanisms contributing to ciprofloxacin resistance among Gram negative bacteria causing infections to cancer patients10.1038/s41598-018-30756-42045-2322https://doaj.org/article/0d34a39e48fa4b6a9a9ac10e7aa954d62018-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-30756-4https://doaj.org/toc/2045-2322Abstract Fluoroquinolones have been used for prophylaxis against infections in cancer patients but their impact on the resistance mechanisms still require further investigation. To elucidate mechanisms underlying ciprofloxacin (CIP) resistance in Gram-negative pathogens causing infections to cancer patients, 169 isolates were investigated. Broth microdilution assays showed high-level CIP resistance in 89.3% of the isolates. Target site mutations were analyzed using PCR and DNA sequencing in 15 selected isolates. Of them, all had gyrA mutations (codons 83 and 87) with parC mutations (codons 80 and 84) in 93.3%. All isolates were screened for plasmid-mediated quinolone resistance (PMQR) genes and 56.8% of them were positive in this respect. Among PMQR genes, aac(6′)-Ib-cr predominated (42.6%) while qnr genes were harbored by 32.5%. This comprised qnrS in 26.6% and qnrB in 6.5%. Clonality of the qnr-positive isolates using ERIC-PCR revealed that most of them were not clonal. CIP MIC reduction by CCCP, an efflux pump inhibitor, was studied and the results revealed that contribution of efflux activity was observed in 18.3% of the isolates. Furthermore, most fluoroquinolone resistance mechanisms were detected among Gram-negative isolates recovered from cancer patients. Target site mutations had the highest impact on CIP resistance as compared to PMQRs and efflux activity.Samira M. HamedWalid F. ElkhatibHadir A. El-MahallawyMai M. HelmyMohamed S. AshourKhaled M. A. AboshanabNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-10 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Samira M. Hamed Walid F. Elkhatib Hadir A. El-Mahallawy Mai M. Helmy Mohamed S. Ashour Khaled M. A. Aboshanab Multiple mechanisms contributing to ciprofloxacin resistance among Gram negative bacteria causing infections to cancer patients
description	Abstract Fluoroquinolones have been used for prophylaxis against infections in cancer patients but their impact on the resistance mechanisms still require further investigation. To elucidate mechanisms underlying ciprofloxacin (CIP) resistance in Gram-negative pathogens causing infections to cancer patients, 169 isolates were investigated. Broth microdilution assays showed high-level CIP resistance in 89.3% of the isolates. Target site mutations were analyzed using PCR and DNA sequencing in 15 selected isolates. Of them, all had gyrA mutations (codons 83 and 87) with parC mutations (codons 80 and 84) in 93.3%. All isolates were screened for plasmid-mediated quinolone resistance (PMQR) genes and 56.8% of them were positive in this respect. Among PMQR genes, aac(6′)-Ib-cr predominated (42.6%) while qnr genes were harbored by 32.5%. This comprised qnrS in 26.6% and qnrB in 6.5%. Clonality of the qnr-positive isolates using ERIC-PCR revealed that most of them were not clonal. CIP MIC reduction by CCCP, an efflux pump inhibitor, was studied and the results revealed that contribution of efflux activity was observed in 18.3% of the isolates. Furthermore, most fluoroquinolone resistance mechanisms were detected among Gram-negative isolates recovered from cancer patients. Target site mutations had the highest impact on CIP resistance as compared to PMQRs and efflux activity.
format	article
author	Samira M. Hamed Walid F. Elkhatib Hadir A. El-Mahallawy Mai M. Helmy Mohamed S. Ashour Khaled M. A. Aboshanab
author_facet	Samira M. Hamed Walid F. Elkhatib Hadir A. El-Mahallawy Mai M. Helmy Mohamed S. Ashour Khaled M. A. Aboshanab
author_sort	Samira M. Hamed
title	Multiple mechanisms contributing to ciprofloxacin resistance among Gram negative bacteria causing infections to cancer patients
title_short	Multiple mechanisms contributing to ciprofloxacin resistance among Gram negative bacteria causing infections to cancer patients
title_full	Multiple mechanisms contributing to ciprofloxacin resistance among Gram negative bacteria causing infections to cancer patients
title_fullStr	Multiple mechanisms contributing to ciprofloxacin resistance among Gram negative bacteria causing infections to cancer patients
title_full_unstemmed	Multiple mechanisms contributing to ciprofloxacin resistance among Gram negative bacteria causing infections to cancer patients
title_sort	multiple mechanisms contributing to ciprofloxacin resistance among gram negative bacteria causing infections to cancer patients
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/0d34a39e48fa4b6a9a9ac10e7aa954d6
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Multiple mechanisms contributing to ciprofloxacin resistance among Gram negative bacteria causing infections to cancer patients

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