Effects of chemically modified nanostructured PLGA on functioning of lung and breast cancer cells

Lijuan Zhang,1 Thomas J Webster21Department of Chemistry, 2School of Engineering, Brown University, Providence, RI, USABackground: The aim of this study was to investigate the effects of poly-lactic-co-glycolic acid (PLGA) nanotopographies with alginate or chitosan protein preadsorption on the funct...

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Autores principales:	Zhang L, Webster TJ
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2013
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/0d361680179f43c8b2b17f93f0b330a9
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spelling	oai:doaj.org-article:0d361680179f43c8b2b17f93f0b330a92021-12-02T01:13:35ZEffects of chemically modified nanostructured PLGA on functioning of lung and breast cancer cells1176-91141178-2013https://doaj.org/article/0d361680179f43c8b2b17f93f0b330a92013-05-01T00:00:00Zhttp://www.dovepress.com/effects-of-chemically-modified-nanostructured-plga-on-functioning-of-l-a13066https://doaj.org/toc/1176-9114https://doaj.org/toc/1178-2013Lijuan Zhang,1 Thomas J Webster21Department of Chemistry, 2School of Engineering, Brown University, Providence, RI, USABackground: The aim of this study was to investigate the effects of poly-lactic-co-glycolic acid (PLGA) nanotopographies with alginate or chitosan protein preadsorption on the functioning of healthy and cancerous lung and breast cells, including adhesion, proliferation, apoptosis, and release of vascular endothelial growth factor (VEGF), which promotes tumor angiogenesis and secretion.Methods: We used a well established cast-mold technique to create nanoscale surface features on PLGA. Some of the nanomodified PLGA films were then exposed to alginate and chitosan. Surface roughness and the presence of protein was confirmed by atomic force microscopy. Surface energy was quantified by contact angle measurement.Results: Nanostructured PLGA surfaces with 23 nm features decreased synthesis of VEGF in both lung and breast cancer cells compared with conventional PLGA. Preadsorbing alginate further decreased cancer cell function, with nanostructured PLGA preadsorbed with alginate achieving the greatest decrease in synthesis of VEGF in both lung and breast cancer cells. In contrast, compared with nonmodified smooth PLGA, healthy cell functions were either not altered (ie, breast) or were enhanced (ie, lung) by use of nanostructured features and alginate or chitosan protein preadsorption.Conclusion: Using this technique, we developed surface nanometric roughness and modification of surface chemistry that could selectively decrease breast and lung cancer cell functioning without the need for chemotherapeutics. This technique requires further study in a wide range of anticancer and regenerative medicine applications.Keywords: breast, lung, cancer, nanotechnology, alginate, chitosanZhang LWebster TJDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2013, Iss default, Pp 1907-1919 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Zhang L Webster TJ Effects of chemically modified nanostructured PLGA on functioning of lung and breast cancer cells
description	Lijuan Zhang,1 Thomas J Webster21Department of Chemistry, 2School of Engineering, Brown University, Providence, RI, USABackground: The aim of this study was to investigate the effects of poly-lactic-co-glycolic acid (PLGA) nanotopographies with alginate or chitosan protein preadsorption on the functioning of healthy and cancerous lung and breast cells, including adhesion, proliferation, apoptosis, and release of vascular endothelial growth factor (VEGF), which promotes tumor angiogenesis and secretion.Methods: We used a well established cast-mold technique to create nanoscale surface features on PLGA. Some of the nanomodified PLGA films were then exposed to alginate and chitosan. Surface roughness and the presence of protein was confirmed by atomic force microscopy. Surface energy was quantified by contact angle measurement.Results: Nanostructured PLGA surfaces with 23 nm features decreased synthesis of VEGF in both lung and breast cancer cells compared with conventional PLGA. Preadsorbing alginate further decreased cancer cell function, with nanostructured PLGA preadsorbed with alginate achieving the greatest decrease in synthesis of VEGF in both lung and breast cancer cells. In contrast, compared with nonmodified smooth PLGA, healthy cell functions were either not altered (ie, breast) or were enhanced (ie, lung) by use of nanostructured features and alginate or chitosan protein preadsorption.Conclusion: Using this technique, we developed surface nanometric roughness and modification of surface chemistry that could selectively decrease breast and lung cancer cell functioning without the need for chemotherapeutics. This technique requires further study in a wide range of anticancer and regenerative medicine applications.Keywords: breast, lung, cancer, nanotechnology, alginate, chitosan
format	article
author	Zhang L Webster TJ
author_facet	Zhang L Webster TJ
author_sort	Zhang L
title	Effects of chemically modified nanostructured PLGA on functioning of lung and breast cancer cells
title_short	Effects of chemically modified nanostructured PLGA on functioning of lung and breast cancer cells
title_full	Effects of chemically modified nanostructured PLGA on functioning of lung and breast cancer cells
title_fullStr	Effects of chemically modified nanostructured PLGA on functioning of lung and breast cancer cells
title_full_unstemmed	Effects of chemically modified nanostructured PLGA on functioning of lung and breast cancer cells
title_sort	effects of chemically modified nanostructured plga on functioning of lung and breast cancer cells
publisher	Dove Medical Press
publishDate	2013
url	https://doaj.org/article/0d361680179f43c8b2b17f93f0b330a9
work_keys_str_mv	AT zhangl effectsofchemicallymodifiednanostructuredplgaonfunctioningoflungandbreastcancercells AT webstertj effectsofchemicallymodifiednanostructuredplgaonfunctioningoflungandbreastcancercells
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Effects of chemically modified nanostructured PLGA on functioning of lung and breast cancer cells

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