Strategy for chemotherapeutic delivery using a nanosized porous metal-organic framework with a central composite design

Yingpeng Li,1 Xiuyan Li,2 Qingxia Guan,2 Chunjing Zhang,2 Ting Xu,2 Yujing Dong,2 Xinyu Bai,2 Weiping Zhang3 1College of Pharmacy, Tianjin University of Traditional Chinese Medicine, Tianjin, 2College of Pharmacy, Heilongjiang University of Traditional Chinese Medicine, Harbin, People’s R...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Li YP, Li XY, Guan QX, Zhang CJ, Xu T, Dong YJ, Bai XY, Zhang WP
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2017
Materias:	5-fluorouracil drug delivery nanoparticles nanoMOFs Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/0d39da1feb6d4579b0a8b8c585a4a6d9
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:0d39da1feb6d4579b0a8b8c585a4a6d9
record_format	dspace
spelling	oai:doaj.org-article:0d39da1feb6d4579b0a8b8c585a4a6d92021-12-02T03:58:31ZStrategy for chemotherapeutic delivery using a nanosized porous metal-organic framework with a central composite design1178-2013https://doaj.org/article/0d39da1feb6d4579b0a8b8c585a4a6d92017-02-01T00:00:00Zhttps://www.dovepress.com/strategy-for-chemotherapeutic-delivery-using-a-nanosized-porous-metal--peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Yingpeng Li,1 Xiuyan Li,2 Qingxia Guan,2 Chunjing Zhang,2 Ting Xu,2 Yujing Dong,2 Xinyu Bai,2 Weiping Zhang3 1College of Pharmacy, Tianjin University of Traditional Chinese Medicine, Tianjin, 2College of Pharmacy, Heilongjiang University of Traditional Chinese Medicine, Harbin, People’s Republic of China; 3Pope John XXIII High School, Everett, MA, USA Background: Enhancing drug delivery is an ongoing endeavor in pharmaceutics, especially when the efficacy of chemotherapy for cancer is concerned. In this study, we prepared and evaluated nanosized HKUST-1 (nanoHKUST-1), nanosized metal-organic drug delivery framework, loaded with 5-fluorouracil (5-FU) for potential use in cancer treatment.Materials and methods: NanoHKUST-1 was prepared by reacting copper (II) acetate [Cu(OAc)2] and benzene-1,3,5-tricarboxylic acid (H3BTC) with benzoic acid (C6H5COOH) at room temperature (23.7°C±2.4°C). A central composite design was used to optimize 5-FU-loaded nanoHKUST-1. Contact time, ethanol concentration, and 5-FU:material ratios were the independent variables, and the entrapment efficiency of 5-FU was the response parameter measured. Powder X-ray diffraction, scanning electron microscopy (SEM), transmission electron microscopy (TEM), and nitrogen adsorption were used to determine the morphology of nanoHKUST-1. In addition, 5-FU release studies were conducted, and the in vitro cytotoxicity was evaluated.Results: Entrapment efficiency and drug loading were 9.96% and 40.22%, respectively, while the small-angle X-ray diffraction patterns confirmed a regular porous structure. The SEM and TEM images of the nanoHKUST-1 confirmed the presence of round particles (diameter: approximately 100 nm) and regular polygon arrays of mesoporous channels of approximately 2–5 nm. The half-maximal lethal concentration (LC50) of the 5-FU-loaded nanoHKUST-1 was approximately 10 µg/mL.Conclusion: The results indicated that nanoHKUST-1 is a potential vector worth developing as a cancer chemotherapeutic drug delivery system. Keywords: 5-fluorouracil, drug delivery, nanoparticles, nano-MOFsLi YPLi XYGuan QXZhang CJXu TDong YJBai XYZhang WPDove Medical Pressarticle5-fluorouracildrug deliverynanoparticlesnanoMOFsMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 12, Pp 1465-1474 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	5-fluorouracil drug delivery nanoparticles nanoMOFs Medicine (General) R5-920
spellingShingle	5-fluorouracil drug delivery nanoparticles nanoMOFs Medicine (General) R5-920 Li YP Li XY Guan QX Zhang CJ Xu T Dong YJ Bai XY Zhang WP Strategy for chemotherapeutic delivery using a nanosized porous metal-organic framework with a central composite design
description	Yingpeng Li,1 Xiuyan Li,2 Qingxia Guan,2 Chunjing Zhang,2 Ting Xu,2 Yujing Dong,2 Xinyu Bai,2 Weiping Zhang3 1College of Pharmacy, Tianjin University of Traditional Chinese Medicine, Tianjin, 2College of Pharmacy, Heilongjiang University of Traditional Chinese Medicine, Harbin, People’s Republic of China; 3Pope John XXIII High School, Everett, MA, USA Background: Enhancing drug delivery is an ongoing endeavor in pharmaceutics, especially when the efficacy of chemotherapy for cancer is concerned. In this study, we prepared and evaluated nanosized HKUST-1 (nanoHKUST-1), nanosized metal-organic drug delivery framework, loaded with 5-fluorouracil (5-FU) for potential use in cancer treatment.Materials and methods: NanoHKUST-1 was prepared by reacting copper (II) acetate [Cu(OAc)2] and benzene-1,3,5-tricarboxylic acid (H3BTC) with benzoic acid (C6H5COOH) at room temperature (23.7°C±2.4°C). A central composite design was used to optimize 5-FU-loaded nanoHKUST-1. Contact time, ethanol concentration, and 5-FU:material ratios were the independent variables, and the entrapment efficiency of 5-FU was the response parameter measured. Powder X-ray diffraction, scanning electron microscopy (SEM), transmission electron microscopy (TEM), and nitrogen adsorption were used to determine the morphology of nanoHKUST-1. In addition, 5-FU release studies were conducted, and the in vitro cytotoxicity was evaluated.Results: Entrapment efficiency and drug loading were 9.96% and 40.22%, respectively, while the small-angle X-ray diffraction patterns confirmed a regular porous structure. The SEM and TEM images of the nanoHKUST-1 confirmed the presence of round particles (diameter: approximately 100 nm) and regular polygon arrays of mesoporous channels of approximately 2–5 nm. The half-maximal lethal concentration (LC50) of the 5-FU-loaded nanoHKUST-1 was approximately 10 µg/mL.Conclusion: The results indicated that nanoHKUST-1 is a potential vector worth developing as a cancer chemotherapeutic drug delivery system. Keywords: 5-fluorouracil, drug delivery, nanoparticles, nano-MOFs
format	article
author	Li YP Li XY Guan QX Zhang CJ Xu T Dong YJ Bai XY Zhang WP
author_facet	Li YP Li XY Guan QX Zhang CJ Xu T Dong YJ Bai XY Zhang WP
author_sort	Li YP
title	Strategy for chemotherapeutic delivery using a nanosized porous metal-organic framework with a central composite design
title_short	Strategy for chemotherapeutic delivery using a nanosized porous metal-organic framework with a central composite design
title_full	Strategy for chemotherapeutic delivery using a nanosized porous metal-organic framework with a central composite design
title_fullStr	Strategy for chemotherapeutic delivery using a nanosized porous metal-organic framework with a central composite design
title_full_unstemmed	Strategy for chemotherapeutic delivery using a nanosized porous metal-organic framework with a central composite design
title_sort	strategy for chemotherapeutic delivery using a nanosized porous metal-organic framework with a central composite design
publisher	Dove Medical Press
publishDate	2017
url	https://doaj.org/article/0d39da1feb6d4579b0a8b8c585a4a6d9
work_keys_str_mv	AT liyp strategyforchemotherapeuticdeliveryusingananosizedporousmetalorganicframeworkwithacentralcompositedesign AT lixy strategyforchemotherapeuticdeliveryusingananosizedporousmetalorganicframeworkwithacentralcompositedesign AT guanqx strategyforchemotherapeuticdeliveryusingananosizedporousmetalorganicframeworkwithacentralcompositedesign AT zhangcj strategyforchemotherapeuticdeliveryusingananosizedporousmetalorganicframeworkwithacentralcompositedesign AT xut strategyforchemotherapeuticdeliveryusingananosizedporousmetalorganicframeworkwithacentralcompositedesign AT dongyj strategyforchemotherapeuticdeliveryusingananosizedporousmetalorganicframeworkwithacentralcompositedesign AT baixy strategyforchemotherapeuticdeliveryusingananosizedporousmetalorganicframeworkwithacentralcompositedesign AT zhangwp strategyforchemotherapeuticdeliveryusingananosizedporousmetalorganicframeworkwithacentralcompositedesign
_version_	1718401471920209920

Strategy for chemotherapeutic delivery using a nanosized porous metal-organic framework with a central composite design

Ejemplares similares