Glycine Ameliorates Endoplasmic Reticulum Stress Induced by Thapsigargin in Porcine Oocytes

The endoplasmic reticulum (ER) is a multifunctional organelle in the cytoplasm that plays important roles in female mammalian reproduction. The endoplasmic reticulum and mitochondria interact to maintain the normal function of cells by maintaining intracellular calcium homeostasis. As proven by prev...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Sicong Yu, Lepeng Gao, Chang Zhang, Yumeng Wang, Hainan Lan, Qianran Chu, Suo Li, Xin Zheng
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	endoplasmic reticulum stress glycine apoptosis oocyte meiotic maturation embryo development (in vitro) Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/0d3b3be1b0054181b520ac70cd4b0964
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:0d3b3be1b0054181b520ac70cd4b0964
record_format	dspace
spelling	oai:doaj.org-article:0d3b3be1b0054181b520ac70cd4b09642021-12-01T19:44:13ZGlycine Ameliorates Endoplasmic Reticulum Stress Induced by Thapsigargin in Porcine Oocytes2296-634X10.3389/fcell.2021.733860https://doaj.org/article/0d3b3be1b0054181b520ac70cd4b09642021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fcell.2021.733860/fullhttps://doaj.org/toc/2296-634XThe endoplasmic reticulum (ER) is a multifunctional organelle in the cytoplasm that plays important roles in female mammalian reproduction. The endoplasmic reticulum and mitochondria interact to maintain the normal function of cells by maintaining intracellular calcium homeostasis. As proven by previous research, glycine (Gly) can regulate the intracellular free calcium concentration ([Ca2+]i) and enhance mitochondrial function to improve oocyte maturation in vitro. The effect of Gly on ER function during oocyte in vitro maturation (IVM) is not clear. In this study, we induced an ER stress model with thapsigargin (TG) to explore whether Gly can reverse the ER stress induced by TG treatment and whether it is associated with calcium regulation. The results showed that the addition of Gly could improve the decrease in the average cumulus diameter, the first polar body excretion rate caused by TG-induced ER stress, the cleavage rate and the blastocyst rate. Gly supplementation could reduce the ER stress induced by TG by significantly improving the ER levels and significantly downregulating the expression of genes related to ER stress (Xbp1, ATF4, and ATF6). Moreover, Gly also significantly alleviated the increase in reactive oxygen species (ROS) levels and the decrease in mitochondrial membrane potential (ΔΨ m) to improve mitochondrial function in porcine oocytes exposed to TG. Furthermore, Gly reduced the [Ca2+]i and mitochondrial Ca2+ ([Ca2+]m) levels and restored the ER Ca2+ ([Ca2+]ER) levels in TG-exposed porcine oocytes. Moreover, we found that the increase in [Ca2+]i may be caused by changes in the distribution and expression of inositol 1,4,5-triphosphate receptor (IP3R1) and voltage-dependent anion channel 1 (VDAC1), while Gly can restore the distribution and expression of IP3R1 and VDAC1 to normal levels. Apoptosis-related indexes (Caspase 3 activity and Annexin-V) and gene expression Bax, Cyto C, and Caspase 3) were significantly increased in the TG group, but they could be restored by adding Gly. Our results suggest that Gly can ameliorate ER stress and apoptosis in TG-exposed porcine oocytes and can further enhance the developmental potential of porcine oocytes in vitro.Sicong YuLepeng GaoChang ZhangYumeng WangHainan LanQianran ChuSuo LiXin ZhengFrontiers Media S.A.articleendoplasmic reticulum stressglycineapoptosisoocyte meiotic maturationembryo development (in vitro)Biology (General)QH301-705.5ENFrontiers in Cell and Developmental Biology, Vol 9 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	endoplasmic reticulum stress glycine apoptosis oocyte meiotic maturation embryo development (in vitro) Biology (General) QH301-705.5
spellingShingle	endoplasmic reticulum stress glycine apoptosis oocyte meiotic maturation embryo development (in vitro) Biology (General) QH301-705.5 Sicong Yu Lepeng Gao Chang Zhang Yumeng Wang Hainan Lan Qianran Chu Suo Li Xin Zheng Glycine Ameliorates Endoplasmic Reticulum Stress Induced by Thapsigargin in Porcine Oocytes
description	The endoplasmic reticulum (ER) is a multifunctional organelle in the cytoplasm that plays important roles in female mammalian reproduction. The endoplasmic reticulum and mitochondria interact to maintain the normal function of cells by maintaining intracellular calcium homeostasis. As proven by previous research, glycine (Gly) can regulate the intracellular free calcium concentration ([Ca2+]i) and enhance mitochondrial function to improve oocyte maturation in vitro. The effect of Gly on ER function during oocyte in vitro maturation (IVM) is not clear. In this study, we induced an ER stress model with thapsigargin (TG) to explore whether Gly can reverse the ER stress induced by TG treatment and whether it is associated with calcium regulation. The results showed that the addition of Gly could improve the decrease in the average cumulus diameter, the first polar body excretion rate caused by TG-induced ER stress, the cleavage rate and the blastocyst rate. Gly supplementation could reduce the ER stress induced by TG by significantly improving the ER levels and significantly downregulating the expression of genes related to ER stress (Xbp1, ATF4, and ATF6). Moreover, Gly also significantly alleviated the increase in reactive oxygen species (ROS) levels and the decrease in mitochondrial membrane potential (ΔΨ m) to improve mitochondrial function in porcine oocytes exposed to TG. Furthermore, Gly reduced the [Ca2+]i and mitochondrial Ca2+ ([Ca2+]m) levels and restored the ER Ca2+ ([Ca2+]ER) levels in TG-exposed porcine oocytes. Moreover, we found that the increase in [Ca2+]i may be caused by changes in the distribution and expression of inositol 1,4,5-triphosphate receptor (IP3R1) and voltage-dependent anion channel 1 (VDAC1), while Gly can restore the distribution and expression of IP3R1 and VDAC1 to normal levels. Apoptosis-related indexes (Caspase 3 activity and Annexin-V) and gene expression Bax, Cyto C, and Caspase 3) were significantly increased in the TG group, but they could be restored by adding Gly. Our results suggest that Gly can ameliorate ER stress and apoptosis in TG-exposed porcine oocytes and can further enhance the developmental potential of porcine oocytes in vitro.
format	article
author	Sicong Yu Lepeng Gao Chang Zhang Yumeng Wang Hainan Lan Qianran Chu Suo Li Xin Zheng
author_facet	Sicong Yu Lepeng Gao Chang Zhang Yumeng Wang Hainan Lan Qianran Chu Suo Li Xin Zheng
author_sort	Sicong Yu
title	Glycine Ameliorates Endoplasmic Reticulum Stress Induced by Thapsigargin in Porcine Oocytes
title_short	Glycine Ameliorates Endoplasmic Reticulum Stress Induced by Thapsigargin in Porcine Oocytes
title_full	Glycine Ameliorates Endoplasmic Reticulum Stress Induced by Thapsigargin in Porcine Oocytes
title_fullStr	Glycine Ameliorates Endoplasmic Reticulum Stress Induced by Thapsigargin in Porcine Oocytes
title_full_unstemmed	Glycine Ameliorates Endoplasmic Reticulum Stress Induced by Thapsigargin in Porcine Oocytes
title_sort	glycine ameliorates endoplasmic reticulum stress induced by thapsigargin in porcine oocytes
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/0d3b3be1b0054181b520ac70cd4b0964
work_keys_str_mv	AT sicongyu glycineamelioratesendoplasmicreticulumstressinducedbythapsigargininporcineoocytes AT lepenggao glycineamelioratesendoplasmicreticulumstressinducedbythapsigargininporcineoocytes AT changzhang glycineamelioratesendoplasmicreticulumstressinducedbythapsigargininporcineoocytes AT yumengwang glycineamelioratesendoplasmicreticulumstressinducedbythapsigargininporcineoocytes AT hainanlan glycineamelioratesendoplasmicreticulumstressinducedbythapsigargininporcineoocytes AT qianranchu glycineamelioratesendoplasmicreticulumstressinducedbythapsigargininporcineoocytes AT suoli glycineamelioratesendoplasmicreticulumstressinducedbythapsigargininporcineoocytes AT xinzheng glycineamelioratesendoplasmicreticulumstressinducedbythapsigargininporcineoocytes
_version_	1718404615842562048

Glycine Ameliorates Endoplasmic Reticulum Stress Induced by Thapsigargin in Porcine Oocytes

Ejemplares similares