Dietary Nano-ZnO Is Absorbed via Endocytosis and ZIP Pathways, Upregulates Lipogenesis, and Induces Lipotoxicity in the Intestine of Yellow Catfish

Nano-sized zinc oxide (nano-ZnO) affects lipid deposition, but its absorption patterns and mechanisms affecting lipid metabolism are still unclear. This study was undertaken to investigate the molecular mechanism of nano-ZnO absorption and its effects on lipid metabolism in the intestinal tissues of...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Shu-Wei Chen, Wu-Hong Lv, Kun Wu, Guang-Hui Chen, Fang Chen, Chang-Chun Song, Zhi Luo
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	nano-ZnO absorption pathway lipid metabolism lipotoxicity vertebrates Biology (General) QH301-705.5 Chemistry QD1-999
Acceso en línea:	https://doaj.org/article/0d3c6374d6cc485e88a39dd544843b2f
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:0d3c6374d6cc485e88a39dd544843b2f
record_format	dspace
spelling	oai:doaj.org-article:0d3c6374d6cc485e88a39dd544843b2f2021-11-11T17:26:35ZDietary Nano-ZnO Is Absorbed via Endocytosis and ZIP Pathways, Upregulates Lipogenesis, and Induces Lipotoxicity in the Intestine of Yellow Catfish10.3390/ijms2221120471422-00671661-6596https://doaj.org/article/0d3c6374d6cc485e88a39dd544843b2f2021-11-01T00:00:00Zhttps://www.mdpi.com/1422-0067/22/21/12047https://doaj.org/toc/1661-6596https://doaj.org/toc/1422-0067Nano-sized zinc oxide (nano-ZnO) affects lipid deposition, but its absorption patterns and mechanisms affecting lipid metabolism are still unclear. This study was undertaken to investigate the molecular mechanism of nano-ZnO absorption and its effects on lipid metabolism in the intestinal tissues of a widely distributed freshwater teleost yellow catfish <i>Pelteobagrus fulvidraco</i>. We found that 100 mg/kg dietary nano-ZnO (H-Zn group) significantly increased intestinal Zn contents. The <i>zip6</i> and <i>zip10</i> mRNA expression levels were higher in the H-Zn group than those in the control (0 mg/kg nano-ZnO), and <i>zip4</i> mRNA abundances were higher in the control than those in the L-Zn (50 mg/kg nano-ZnO) and H-Zn groups. Eps15, <i>dynamin1</i>, <i>dynamin2</i>, <i>caveolin1,</i> and <i>caveolin2</i> mRNA expression levels tended to reduce with dietary nano-ZnO addition. Dietary nano-ZnO increased triglyceride (TG) content and the activities of the lipogenic enzymes glucose 6-phosphate dehydrogenase (G6PD), 6-phosphogluconate dehydrogenase (6PGD), and isocitrate dehydrogenase (ICDH), upregulated the mRNA abundances of lipogenic genes <i>6pgd</i>, <i>fatty acid synthase (fas),</i> and <i>sterol regulatory element binding protein 1 (srebp1)</i>, and reduced the mRNA expression of <i>farnesoid X receptor (fxr)</i> and small heterodimer partner (<i>shp</i>). The SHP protein level in the H-Zn group was lower than that in the control and the L-Zn group markedly. Our in vitro study indicated that the intestinal epithelial cells (IECs) absorbed nano-ZnO via endocytosis, and nano-Zn-induced TG deposition and lipogenesis were partially attributable to the endocytosis of nano-ZnO in IECs. Mechanistically, nano-ZnO-induced TG deposition was closely related to the metal responsive transcription factor 1 (MTF-1)-SHP pathway. Thus, for the first time, we found that the lipogenesis effects of nano-ZnO probably depended on the key gene <i>shp,</i> which is potentially regulated by MTF1 and/or FXR. This novel signaling pathway of MTF-1 through SHP may be relevant to explain the toxic effects and lipotoxicity ascribed to dietary nano-ZnO addition.Shu-Wei ChenWu-Hong LvKun WuGuang-Hui ChenFang ChenChang-Chun SongZhi LuoMDPI AGarticlenano-ZnOabsorption pathwaylipid metabolismlipotoxicityvertebratesBiology (General)QH301-705.5ChemistryQD1-999ENInternational Journal of Molecular Sciences, Vol 22, Iss 12047, p 12047 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	nano-ZnO absorption pathway lipid metabolism lipotoxicity vertebrates Biology (General) QH301-705.5 Chemistry QD1-999
spellingShingle	nano-ZnO absorption pathway lipid metabolism lipotoxicity vertebrates Biology (General) QH301-705.5 Chemistry QD1-999 Shu-Wei Chen Wu-Hong Lv Kun Wu Guang-Hui Chen Fang Chen Chang-Chun Song Zhi Luo Dietary Nano-ZnO Is Absorbed via Endocytosis and ZIP Pathways, Upregulates Lipogenesis, and Induces Lipotoxicity in the Intestine of Yellow Catfish
description	Nano-sized zinc oxide (nano-ZnO) affects lipid deposition, but its absorption patterns and mechanisms affecting lipid metabolism are still unclear. This study was undertaken to investigate the molecular mechanism of nano-ZnO absorption and its effects on lipid metabolism in the intestinal tissues of a widely distributed freshwater teleost yellow catfish <i>Pelteobagrus fulvidraco</i>. We found that 100 mg/kg dietary nano-ZnO (H-Zn group) significantly increased intestinal Zn contents. The <i>zip6</i> and <i>zip10</i> mRNA expression levels were higher in the H-Zn group than those in the control (0 mg/kg nano-ZnO), and <i>zip4</i> mRNA abundances were higher in the control than those in the L-Zn (50 mg/kg nano-ZnO) and H-Zn groups. Eps15, <i>dynamin1</i>, <i>dynamin2</i>, <i>caveolin1,</i> and <i>caveolin2</i> mRNA expression levels tended to reduce with dietary nano-ZnO addition. Dietary nano-ZnO increased triglyceride (TG) content and the activities of the lipogenic enzymes glucose 6-phosphate dehydrogenase (G6PD), 6-phosphogluconate dehydrogenase (6PGD), and isocitrate dehydrogenase (ICDH), upregulated the mRNA abundances of lipogenic genes <i>6pgd</i>, <i>fatty acid synthase (fas),</i> and <i>sterol regulatory element binding protein 1 (srebp1)</i>, and reduced the mRNA expression of <i>farnesoid X receptor (fxr)</i> and small heterodimer partner (<i>shp</i>). The SHP protein level in the H-Zn group was lower than that in the control and the L-Zn group markedly. Our in vitro study indicated that the intestinal epithelial cells (IECs) absorbed nano-ZnO via endocytosis, and nano-Zn-induced TG deposition and lipogenesis were partially attributable to the endocytosis of nano-ZnO in IECs. Mechanistically, nano-ZnO-induced TG deposition was closely related to the metal responsive transcription factor 1 (MTF-1)-SHP pathway. Thus, for the first time, we found that the lipogenesis effects of nano-ZnO probably depended on the key gene <i>shp,</i> which is potentially regulated by MTF1 and/or FXR. This novel signaling pathway of MTF-1 through SHP may be relevant to explain the toxic effects and lipotoxicity ascribed to dietary nano-ZnO addition.
format	article
author	Shu-Wei Chen Wu-Hong Lv Kun Wu Guang-Hui Chen Fang Chen Chang-Chun Song Zhi Luo
author_facet	Shu-Wei Chen Wu-Hong Lv Kun Wu Guang-Hui Chen Fang Chen Chang-Chun Song Zhi Luo
author_sort	Shu-Wei Chen
title	Dietary Nano-ZnO Is Absorbed via Endocytosis and ZIP Pathways, Upregulates Lipogenesis, and Induces Lipotoxicity in the Intestine of Yellow Catfish
title_short	Dietary Nano-ZnO Is Absorbed via Endocytosis and ZIP Pathways, Upregulates Lipogenesis, and Induces Lipotoxicity in the Intestine of Yellow Catfish
title_full	Dietary Nano-ZnO Is Absorbed via Endocytosis and ZIP Pathways, Upregulates Lipogenesis, and Induces Lipotoxicity in the Intestine of Yellow Catfish
title_fullStr	Dietary Nano-ZnO Is Absorbed via Endocytosis and ZIP Pathways, Upregulates Lipogenesis, and Induces Lipotoxicity in the Intestine of Yellow Catfish
title_full_unstemmed	Dietary Nano-ZnO Is Absorbed via Endocytosis and ZIP Pathways, Upregulates Lipogenesis, and Induces Lipotoxicity in the Intestine of Yellow Catfish
title_sort	dietary nano-zno is absorbed via endocytosis and zip pathways, upregulates lipogenesis, and induces lipotoxicity in the intestine of yellow catfish
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/0d3c6374d6cc485e88a39dd544843b2f
work_keys_str_mv	AT shuweichen dietarynanoznoisabsorbedviaendocytosisandzippathwaysupregulateslipogenesisandinduceslipotoxicityintheintestineofyellowcatfish AT wuhonglv dietarynanoznoisabsorbedviaendocytosisandzippathwaysupregulateslipogenesisandinduceslipotoxicityintheintestineofyellowcatfish AT kunwu dietarynanoznoisabsorbedviaendocytosisandzippathwaysupregulateslipogenesisandinduceslipotoxicityintheintestineofyellowcatfish AT guanghuichen dietarynanoznoisabsorbedviaendocytosisandzippathwaysupregulateslipogenesisandinduceslipotoxicityintheintestineofyellowcatfish AT fangchen dietarynanoznoisabsorbedviaendocytosisandzippathwaysupregulateslipogenesisandinduceslipotoxicityintheintestineofyellowcatfish AT changchunsong dietarynanoznoisabsorbedviaendocytosisandzippathwaysupregulateslipogenesisandinduceslipotoxicityintheintestineofyellowcatfish AT zhiluo dietarynanoznoisabsorbedviaendocytosisandzippathwaysupregulateslipogenesisandinduceslipotoxicityintheintestineofyellowcatfish
_version_	1718432044131811328

Dietary Nano-ZnO Is Absorbed via Endocytosis and ZIP Pathways, Upregulates Lipogenesis, and Induces Lipotoxicity in the Intestine of Yellow Catfish

Ejemplares similares