Secretome Profiling of Atlantic Salmon Head Kidney Leukocytes Highlights the Role of Phagocytes in the Immune Response to Soluble β-Glucan

β‐Glucans (BG) are glucose polymers which are produced in bacteria and fungi but not in vertebrate organisms. Being recognized by phagocytic leukocytes including macrophages and neutrophils through receptors such as dectin-1 and Complement receptor 3 (CR3), the BG are perceived by the innate immune...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Dimitar B. Iliev, Guro Strandskog, Mehrdad Sobhkhez, Jack A. Bruun, Jorunn B. Jørgensen
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	β‐Glucan Atlantic salmon leukocytes mononuclear phagocytes polymorphonuclear granulocytes extracellular vesicles Immunologic diseases. Allergy RC581-607
Acceso en línea:	https://doaj.org/article/0d3db533cfb3459185acf04956ac358d
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:0d3db533cfb3459185acf04956ac358d
record_format	dspace
spelling	oai:doaj.org-article:0d3db533cfb3459185acf04956ac358d2021-12-01T19:39:32ZSecretome Profiling of Atlantic Salmon Head Kidney Leukocytes Highlights the Role of Phagocytes in the Immune Response to Soluble β-Glucan1664-322410.3389/fimmu.2021.736964https://doaj.org/article/0d3db533cfb3459185acf04956ac358d2021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fimmu.2021.736964/fullhttps://doaj.org/toc/1664-3224β‐Glucans (BG) are glucose polymers which are produced in bacteria and fungi but not in vertebrate organisms. Being recognized by phagocytic leukocytes including macrophages and neutrophils through receptors such as dectin-1 and Complement receptor 3 (CR3), the BG are perceived by the innate immune system of vertebrates as foreign substances known as Pathogen Associated Molecular Patterns (PAMPs). The yeast-derived BG has been recognized for its potent biological activity and it is used as an immunomodulator in human and veterinary medicine. The goal of the current study was to characterize the immunostimulatory activity of soluble yeast BG in primary cultures of Atlantic salmon (Salmo salar) head kidney leukocytes (HKLs) in which phagocytic cell types including neutrophils and mononuclear phagocytes predominate. The effect of BG on the secretome of HKL cultures, including secretion of extracellular vesicles (EVs) and soluble protein55s was characterized through western blotting and mass spectrometry. The results demonstrate that, along with upregulation of proinflammatory genes, BG induces secretion of ubiquitinated proteins (UbP), MHCII-containing EVs from professional antigen presenting cells as well as proteins derived from granules of polymorphonuclear granulocytes (PMN). Among the most abundant proteins identified in BG-induced EVs were beta-2 integrin subunits, including CD18 and CD11 homologs, which highlights the role of salmon granulocytes and mononuclear phagocytes in the response to soluble BG. Overall, the current work advances the knowledge about the immunostimulatory activity of yeast BG on the salmon immune system by shedding light on the effect of this PAMP on the secretome of salmon leukocytes.Dimitar B. IlievDimitar B. IlievGuro StrandskogMehrdad SobhkhezJack A. BruunJorunn B. JørgensenFrontiers Media S.A.articleβ‐GlucanAtlantic salmonleukocytesmononuclear phagocytespolymorphonuclear granulocytesextracellular vesiclesImmunologic diseases. AllergyRC581-607ENFrontiers in Immunology, Vol 12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	β‐Glucan Atlantic salmon leukocytes mononuclear phagocytes polymorphonuclear granulocytes extracellular vesicles Immunologic diseases. Allergy RC581-607
spellingShingle	β‐Glucan Atlantic salmon leukocytes mononuclear phagocytes polymorphonuclear granulocytes extracellular vesicles Immunologic diseases. Allergy RC581-607 Dimitar B. Iliev Dimitar B. Iliev Guro Strandskog Mehrdad Sobhkhez Jack A. Bruun Jorunn B. Jørgensen Secretome Profiling of Atlantic Salmon Head Kidney Leukocytes Highlights the Role of Phagocytes in the Immune Response to Soluble β-Glucan
description	β‐Glucans (BG) are glucose polymers which are produced in bacteria and fungi but not in vertebrate organisms. Being recognized by phagocytic leukocytes including macrophages and neutrophils through receptors such as dectin-1 and Complement receptor 3 (CR3), the BG are perceived by the innate immune system of vertebrates as foreign substances known as Pathogen Associated Molecular Patterns (PAMPs). The yeast-derived BG has been recognized for its potent biological activity and it is used as an immunomodulator in human and veterinary medicine. The goal of the current study was to characterize the immunostimulatory activity of soluble yeast BG in primary cultures of Atlantic salmon (Salmo salar) head kidney leukocytes (HKLs) in which phagocytic cell types including neutrophils and mononuclear phagocytes predominate. The effect of BG on the secretome of HKL cultures, including secretion of extracellular vesicles (EVs) and soluble protein55s was characterized through western blotting and mass spectrometry. The results demonstrate that, along with upregulation of proinflammatory genes, BG induces secretion of ubiquitinated proteins (UbP), MHCII-containing EVs from professional antigen presenting cells as well as proteins derived from granules of polymorphonuclear granulocytes (PMN). Among the most abundant proteins identified in BG-induced EVs were beta-2 integrin subunits, including CD18 and CD11 homologs, which highlights the role of salmon granulocytes and mononuclear phagocytes in the response to soluble BG. Overall, the current work advances the knowledge about the immunostimulatory activity of yeast BG on the salmon immune system by shedding light on the effect of this PAMP on the secretome of salmon leukocytes.
format	article
author	Dimitar B. Iliev Dimitar B. Iliev Guro Strandskog Mehrdad Sobhkhez Jack A. Bruun Jorunn B. Jørgensen
author_facet	Dimitar B. Iliev Dimitar B. Iliev Guro Strandskog Mehrdad Sobhkhez Jack A. Bruun Jorunn B. Jørgensen
author_sort	Dimitar B. Iliev
title	Secretome Profiling of Atlantic Salmon Head Kidney Leukocytes Highlights the Role of Phagocytes in the Immune Response to Soluble β-Glucan
title_short	Secretome Profiling of Atlantic Salmon Head Kidney Leukocytes Highlights the Role of Phagocytes in the Immune Response to Soluble β-Glucan
title_full	Secretome Profiling of Atlantic Salmon Head Kidney Leukocytes Highlights the Role of Phagocytes in the Immune Response to Soluble β-Glucan
title_fullStr	Secretome Profiling of Atlantic Salmon Head Kidney Leukocytes Highlights the Role of Phagocytes in the Immune Response to Soluble β-Glucan
title_full_unstemmed	Secretome Profiling of Atlantic Salmon Head Kidney Leukocytes Highlights the Role of Phagocytes in the Immune Response to Soluble β-Glucan
title_sort	secretome profiling of atlantic salmon head kidney leukocytes highlights the role of phagocytes in the immune response to soluble β-glucan
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/0d3db533cfb3459185acf04956ac358d
work_keys_str_mv	AT dimitarbiliev secretomeprofilingofatlanticsalmonheadkidneyleukocyteshighlightstheroleofphagocytesintheimmuneresponsetosolublebglucan AT dimitarbiliev secretomeprofilingofatlanticsalmonheadkidneyleukocyteshighlightstheroleofphagocytesintheimmuneresponsetosolublebglucan AT gurostrandskog secretomeprofilingofatlanticsalmonheadkidneyleukocyteshighlightstheroleofphagocytesintheimmuneresponsetosolublebglucan AT mehrdadsobhkhez secretomeprofilingofatlanticsalmonheadkidneyleukocyteshighlightstheroleofphagocytesintheimmuneresponsetosolublebglucan AT jackabruun secretomeprofilingofatlanticsalmonheadkidneyleukocyteshighlightstheroleofphagocytesintheimmuneresponsetosolublebglucan AT jorunnbjørgensen secretomeprofilingofatlanticsalmonheadkidneyleukocyteshighlightstheroleofphagocytesintheimmuneresponsetosolublebglucan
_version_	1718404616037597184

Secretome Profiling of Atlantic Salmon Head Kidney Leukocytes Highlights the Role of Phagocytes in the Immune Response to Soluble β-Glucan

Ejemplares similares