CD160-associated CD8 T-cell functional impairment is independent of PD-1 expression.
Expression of co-inhibitory molecules is generally associated with T-cell dysfunction in chronic viral infections such as HIV or HCV. However, their relative contribution in the T-cell impairment remains unclear. In the present study, we have evaluated the impact of the expression of co-inhibitory m...
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2014
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oai:doaj.org-article:0d4231de15594763a6b82620d3fef77a2021-11-25T05:45:59ZCD160-associated CD8 T-cell functional impairment is independent of PD-1 expression.1553-73661553-737410.1371/journal.ppat.1004380https://doaj.org/article/0d4231de15594763a6b82620d3fef77a2014-09-01T00:00:00Zhttps://doi.org/10.1371/journal.ppat.1004380https://doaj.org/toc/1553-7366https://doaj.org/toc/1553-7374Expression of co-inhibitory molecules is generally associated with T-cell dysfunction in chronic viral infections such as HIV or HCV. However, their relative contribution in the T-cell impairment remains unclear. In the present study, we have evaluated the impact of the expression of co-inhibitory molecules such as 2B4, PD-1 and CD160 on the functions of CD8 T-cells specific to influenza, EBV and CMV. We show that CD8 T-cell populations expressing CD160, but not PD-1, had reduced proliferation capacity and perforin expression, thus indicating that the functional impairment in CD160(+) CD8 T cells may be independent of PD-1 expression. The blockade of CD160/CD160-ligand interaction restored CD8 T-cell proliferation capacity, and the extent of restoration directly correlated with the ex vivo proportion of CD160(+) CD8 T cells suggesting that CD160 negatively regulates TCR-mediated signaling. Furthermore, CD160 expression was not up-regulated upon T-cell activation or proliferation as compared to PD-1. Taken together, these results provide evidence that CD160-associated CD8 T-cell functional impairment is independent of PD-1 expression.Selena ViganòRiddhima BangaFlorence BellangerCéline PellatonAlex FarinaDenis ComteAlexandre HarariMatthieu PerreauPublic Library of Science (PLoS)articleImmunologic diseases. AllergyRC581-607Biology (General)QH301-705.5ENPLoS Pathogens, Vol 10, Iss 9, p e1004380 (2014) |
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DOAJ |
| collection |
DOAJ |
| language |
EN |
| topic |
Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5 |
| spellingShingle |
Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5 Selena Viganò Riddhima Banga Florence Bellanger Céline Pellaton Alex Farina Denis Comte Alexandre Harari Matthieu Perreau CD160-associated CD8 T-cell functional impairment is independent of PD-1 expression. |
| description |
Expression of co-inhibitory molecules is generally associated with T-cell dysfunction in chronic viral infections such as HIV or HCV. However, their relative contribution in the T-cell impairment remains unclear. In the present study, we have evaluated the impact of the expression of co-inhibitory molecules such as 2B4, PD-1 and CD160 on the functions of CD8 T-cells specific to influenza, EBV and CMV. We show that CD8 T-cell populations expressing CD160, but not PD-1, had reduced proliferation capacity and perforin expression, thus indicating that the functional impairment in CD160(+) CD8 T cells may be independent of PD-1 expression. The blockade of CD160/CD160-ligand interaction restored CD8 T-cell proliferation capacity, and the extent of restoration directly correlated with the ex vivo proportion of CD160(+) CD8 T cells suggesting that CD160 negatively regulates TCR-mediated signaling. Furthermore, CD160 expression was not up-regulated upon T-cell activation or proliferation as compared to PD-1. Taken together, these results provide evidence that CD160-associated CD8 T-cell functional impairment is independent of PD-1 expression. |
| format |
article |
| author |
Selena Viganò Riddhima Banga Florence Bellanger Céline Pellaton Alex Farina Denis Comte Alexandre Harari Matthieu Perreau |
| author_facet |
Selena Viganò Riddhima Banga Florence Bellanger Céline Pellaton Alex Farina Denis Comte Alexandre Harari Matthieu Perreau |
| author_sort |
Selena Viganò |
| title |
CD160-associated CD8 T-cell functional impairment is independent of PD-1 expression. |
| title_short |
CD160-associated CD8 T-cell functional impairment is independent of PD-1 expression. |
| title_full |
CD160-associated CD8 T-cell functional impairment is independent of PD-1 expression. |
| title_fullStr |
CD160-associated CD8 T-cell functional impairment is independent of PD-1 expression. |
| title_full_unstemmed |
CD160-associated CD8 T-cell functional impairment is independent of PD-1 expression. |
| title_sort |
cd160-associated cd8 t-cell functional impairment is independent of pd-1 expression. |
| publisher |
Public Library of Science (PLoS) |
| publishDate |
2014 |
| url |
https://doaj.org/article/0d4231de15594763a6b82620d3fef77a |
| work_keys_str_mv |
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