Intestinal Dysbiosis Associated with Systemic Lupus Erythematosus

ABSTRACT Systemic lupus erythematosus (SLE) is the prototypical systemic autoimmune disease in humans and is characterized by the presence of hyperactive immune cells and aberrant antibody responses to nuclear and cytoplasmic antigens, including characteristic anti–double-stranded DNA antibodies. We...

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Autores principales:	Arancha Hevia, Christian Milani, Patricia López, Adriana Cuervo, Silvia Arboleya, Sabrina Duranti, Francesca Turroni, Sonia González, Ana Suárez, Miguel Gueimonde, Marco Ventura, Borja Sánchez, Abelardo Margolles
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2014
Materias:	Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/0d47767074f34d26b887e4a9f3961c69
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spelling	oai:doaj.org-article:0d47767074f34d26b887e4a9f3961c692021-11-15T15:45:54ZIntestinal Dysbiosis Associated with Systemic Lupus Erythematosus10.1128/mBio.01548-142150-7511https://doaj.org/article/0d47767074f34d26b887e4a9f3961c692014-10-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mBio.01548-14https://doaj.org/toc/2150-7511ABSTRACT Systemic lupus erythematosus (SLE) is the prototypical systemic autoimmune disease in humans and is characterized by the presence of hyperactive immune cells and aberrant antibody responses to nuclear and cytoplasmic antigens, including characteristic anti–double-stranded DNA antibodies. We performed a cross-sectional study in order to determine if an SLE-associated gut dysbiosis exists in patients without active disease. A group of 20 SLE patients in remission, for which there was strict inclusion and exclusion criteria, was recruited, and we used an optimized Ion Torrent 16S rRNA gene-based analysis protocol to decipher the fecal microbial profiles of these patients and compare them with those of 20 age- and sex-matched healthy control subjects. We found diversity to be comparable based on Shannon’s index. However, we saw a significantly lower Firmicutes/Bacteroidetes ratio in SLE individuals (median ratio, 1.97) than in healthy subjects (median ratio, 4.86; P < 0.002). A lower Firmicutes/Bacteroidetes ratio in SLE individuals was corroborated by quantitative PCR analysis. Notably, a decrease of some Firmicutes families was also detected. This dysbiosis is reflected, based on in silico functional inference, in an overrepresentation of oxidative phosphorylation and glycan utilization pathways in SLE patient microbiota. IMPORTANCE Growing evidence suggests that the gut microbiota might impact symptoms and progression of some autoimmune diseases. However, how and why this microbial community influences SLE remains to be elucidated. This is the first report describing an SLE-associated intestinal dysbiosis, and it contributes to the understanding of the interplay between the intestinal microbiota and the host in autoimmune disorders.Arancha HeviaChristian MilaniPatricia LópezAdriana CuervoSilvia ArboleyaSabrina DurantiFrancesca TurroniSonia GonzálezAna SuárezMiguel GueimondeMarco VenturaBorja SánchezAbelardo MargollesAmerican Society for MicrobiologyarticleMicrobiologyQR1-502ENmBio, Vol 5, Iss 5 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Microbiology QR1-502
spellingShingle	Microbiology QR1-502 Arancha Hevia Christian Milani Patricia López Adriana Cuervo Silvia Arboleya Sabrina Duranti Francesca Turroni Sonia González Ana Suárez Miguel Gueimonde Marco Ventura Borja Sánchez Abelardo Margolles Intestinal Dysbiosis Associated with Systemic Lupus Erythematosus
description	ABSTRACT Systemic lupus erythematosus (SLE) is the prototypical systemic autoimmune disease in humans and is characterized by the presence of hyperactive immune cells and aberrant antibody responses to nuclear and cytoplasmic antigens, including characteristic anti–double-stranded DNA antibodies. We performed a cross-sectional study in order to determine if an SLE-associated gut dysbiosis exists in patients without active disease. A group of 20 SLE patients in remission, for which there was strict inclusion and exclusion criteria, was recruited, and we used an optimized Ion Torrent 16S rRNA gene-based analysis protocol to decipher the fecal microbial profiles of these patients and compare them with those of 20 age- and sex-matched healthy control subjects. We found diversity to be comparable based on Shannon’s index. However, we saw a significantly lower Firmicutes/Bacteroidetes ratio in SLE individuals (median ratio, 1.97) than in healthy subjects (median ratio, 4.86; P < 0.002). A lower Firmicutes/Bacteroidetes ratio in SLE individuals was corroborated by quantitative PCR analysis. Notably, a decrease of some Firmicutes families was also detected. This dysbiosis is reflected, based on in silico functional inference, in an overrepresentation of oxidative phosphorylation and glycan utilization pathways in SLE patient microbiota. IMPORTANCE Growing evidence suggests that the gut microbiota might impact symptoms and progression of some autoimmune diseases. However, how and why this microbial community influences SLE remains to be elucidated. This is the first report describing an SLE-associated intestinal dysbiosis, and it contributes to the understanding of the interplay between the intestinal microbiota and the host in autoimmune disorders.
format	article
author	Arancha Hevia Christian Milani Patricia López Adriana Cuervo Silvia Arboleya Sabrina Duranti Francesca Turroni Sonia González Ana Suárez Miguel Gueimonde Marco Ventura Borja Sánchez Abelardo Margolles
author_facet	Arancha Hevia Christian Milani Patricia López Adriana Cuervo Silvia Arboleya Sabrina Duranti Francesca Turroni Sonia González Ana Suárez Miguel Gueimonde Marco Ventura Borja Sánchez Abelardo Margolles
author_sort	Arancha Hevia
title	Intestinal Dysbiosis Associated with Systemic Lupus Erythematosus
title_short	Intestinal Dysbiosis Associated with Systemic Lupus Erythematosus
title_full	Intestinal Dysbiosis Associated with Systemic Lupus Erythematosus
title_fullStr	Intestinal Dysbiosis Associated with Systemic Lupus Erythematosus
title_full_unstemmed	Intestinal Dysbiosis Associated with Systemic Lupus Erythematosus
title_sort	intestinal dysbiosis associated with systemic lupus erythematosus
publisher	American Society for Microbiology
publishDate	2014
url	https://doaj.org/article/0d47767074f34d26b887e4a9f3961c69
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Intestinal Dysbiosis Associated with Systemic Lupus Erythematosus

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