Transcription Factor Antagonism Controls Enteroendocrine Cell Specification from Intestinal Stem Cells

Abstract The balanced maintenance and differentiation of local stem cells is required for Homeostatic renewal of tissues. In the Drosophila midgut, the transcription factor Escargot (Esg) maintains undifferentiated states in intestinal stem cells, whereas the transcription factors Scute (Sc) and Pro...

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Autores principales:	Yumei Li, Zhimin Pang, Huanwei Huang, Chenhui Wang, Tao Cai, Rongwen Xi
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/0d50718730f44815a12f3e844a13d360
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spelling	oai:doaj.org-article:0d50718730f44815a12f3e844a13d3602021-12-02T15:05:24ZTranscription Factor Antagonism Controls Enteroendocrine Cell Specification from Intestinal Stem Cells10.1038/s41598-017-01138-z2045-2322https://doaj.org/article/0d50718730f44815a12f3e844a13d3602017-04-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-01138-zhttps://doaj.org/toc/2045-2322Abstract The balanced maintenance and differentiation of local stem cells is required for Homeostatic renewal of tissues. In the Drosophila midgut, the transcription factor Escargot (Esg) maintains undifferentiated states in intestinal stem cells, whereas the transcription factors Scute (Sc) and Prospero (Pros) promote enteroendocrine cell specification. However, the mechanism through which Esg and Sc/Pros coordinately regulate stem cell differentiation is unknown. Here, by combining chromatin immunoprecipitation analysis with genetic studies, we show that both Esg and Sc bind to a common promoter region of pros. Moreover, antagonistic activity between Esg and Sc controls the expression status of Pros in stem cells, thereby, specifying whether stem cells remain undifferentiated or commit to enteroendocrine cell differentiation. Our study therefore reveals transcription factor antagonism between Esg and Sc as a novel mechanism that underlies fate specification from intestinal stem cells in Drosophila.Yumei LiZhimin PangHuanwei HuangChenhui WangTao CaiRongwen XiNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-12 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Yumei Li Zhimin Pang Huanwei Huang Chenhui Wang Tao Cai Rongwen Xi Transcription Factor Antagonism Controls Enteroendocrine Cell Specification from Intestinal Stem Cells
description	Abstract The balanced maintenance and differentiation of local stem cells is required for Homeostatic renewal of tissues. In the Drosophila midgut, the transcription factor Escargot (Esg) maintains undifferentiated states in intestinal stem cells, whereas the transcription factors Scute (Sc) and Prospero (Pros) promote enteroendocrine cell specification. However, the mechanism through which Esg and Sc/Pros coordinately regulate stem cell differentiation is unknown. Here, by combining chromatin immunoprecipitation analysis with genetic studies, we show that both Esg and Sc bind to a common promoter region of pros. Moreover, antagonistic activity between Esg and Sc controls the expression status of Pros in stem cells, thereby, specifying whether stem cells remain undifferentiated or commit to enteroendocrine cell differentiation. Our study therefore reveals transcription factor antagonism between Esg and Sc as a novel mechanism that underlies fate specification from intestinal stem cells in Drosophila.
format	article
author	Yumei Li Zhimin Pang Huanwei Huang Chenhui Wang Tao Cai Rongwen Xi
author_facet	Yumei Li Zhimin Pang Huanwei Huang Chenhui Wang Tao Cai Rongwen Xi
author_sort	Yumei Li
title	Transcription Factor Antagonism Controls Enteroendocrine Cell Specification from Intestinal Stem Cells
title_short	Transcription Factor Antagonism Controls Enteroendocrine Cell Specification from Intestinal Stem Cells
title_full	Transcription Factor Antagonism Controls Enteroendocrine Cell Specification from Intestinal Stem Cells
title_fullStr	Transcription Factor Antagonism Controls Enteroendocrine Cell Specification from Intestinal Stem Cells
title_full_unstemmed	Transcription Factor Antagonism Controls Enteroendocrine Cell Specification from Intestinal Stem Cells
title_sort	transcription factor antagonism controls enteroendocrine cell specification from intestinal stem cells
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/0d50718730f44815a12f3e844a13d360
work_keys_str_mv	AT yumeili transcriptionfactorantagonismcontrolsenteroendocrinecellspecificationfromintestinalstemcells AT zhiminpang transcriptionfactorantagonismcontrolsenteroendocrinecellspecificationfromintestinalstemcells AT huanweihuang transcriptionfactorantagonismcontrolsenteroendocrinecellspecificationfromintestinalstemcells AT chenhuiwang transcriptionfactorantagonismcontrolsenteroendocrinecellspecificationfromintestinalstemcells AT taocai transcriptionfactorantagonismcontrolsenteroendocrinecellspecificationfromintestinalstemcells AT rongwenxi transcriptionfactorantagonismcontrolsenteroendocrinecellspecificationfromintestinalstemcells
_version_	1718388812539756544

Transcription Factor Antagonism Controls Enteroendocrine Cell Specification from Intestinal Stem Cells

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