Co-loading antioxidant N-acetylcysteine attenuates cytotoxicity of iron oxide nanoparticles in hypoxia/reoxygenation cardiomyocytes

Yunli Shen,1,* Shiyu Gong,1,* Jiming Li,1,* Yunkai Wang,1 Xumin Zhang,1 Hao Zheng,1 Qi Zhang,1 Jieyun You,1 Zheyong Huang,2 Yihan Chen11Department of Cardiology, Shanghai East Hospital, Tongji University School of Medicine, Shanghai 200120, People’s Republic of China; 2Shanghai Institute o...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Shen Y, Gong S, Li J, Wang Y, Zhang X, Zheng H, Zhang Q, You J, Huang Z, Chen Y
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2019
Materias:	N-acetylcysteine (NAC) iron oxide nanoparticles Oxidative stress Cardiomyocytes Hypoxia-Reoxygenation Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/0d555e335bf2419fbd28b22741c3b203
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:0d555e335bf2419fbd28b22741c3b203
record_format	dspace
spelling	oai:doaj.org-article:0d555e335bf2419fbd28b22741c3b2032021-12-02T09:15:24ZCo-loading antioxidant N-acetylcysteine attenuates cytotoxicity of iron oxide nanoparticles in hypoxia/reoxygenation cardiomyocytes1178-2013https://doaj.org/article/0d555e335bf2419fbd28b22741c3b2032019-08-01T00:00:00Zhttps://www.dovepress.com/co-loading-antioxidant-n-acetylcysteine-attenuates-cytotoxicity-of-iro-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Yunli Shen,1,* Shiyu Gong,1,* Jiming Li,1,* Yunkai Wang,1 Xumin Zhang,1 Hao Zheng,1 Qi Zhang,1 Jieyun You,1 Zheyong Huang,2 Yihan Chen11Department of Cardiology, Shanghai East Hospital, Tongji University School of Medicine, Shanghai 200120, People’s Republic of China; 2Shanghai Institute of Cardiovascular Diseases, Zhongshan Hospital, Fudan University, Shanghai 200032, People’s Republic of China*These authors contributed equally to this workPurpose: Myocardial delivery of magnetic iron oxide nanoparticles (MNPs) might produce iron overload-induced myocardial injury, and the oxidative stress was regarded as the main mechanism. Therefore, we speculated antioxidant modification might be a reasonable strategy to mitigate the toxicity of MNPs.Methods and results: Antioxidant N-acetylcysteine (NAC) was loaded into magnetic mesoporous silica coated Fe3O4 nanoparticles. Neonatal rat hypoxia/reoxygenation (H/R) cardiomyocytes were incubated with nanoparticles for 24 hrs. NAC can effectively mitigate iron-induced oxidative injury of cardiomyocytes, evidenced by reduced production of MDA, 8-iso-PGF2α, and 8-OHDG and maintained concentrations of SOD, CAT, GSH-Px, and GSH in ELISA and biochemical tests; downregulated expression of CHOP, GRP78, p62, and LC3-II proteins in Western Blot, and less cardiomyocytes apoptosis in flow cytometric analysis.Conclusions: NAC modifying could suppress the toxic effects of Fe3O4 nanoparticles in H/R cardiomyocytes model in vitro, indicating a promising strategy to improve the safety of iron oxide nanoparticles.Keywords: N-acetylcysteine (NAC), iron oxide nanoparticles, oxidative stress, cardiomyocytes, hypoxia-reoxygenationShen YGong SLi JWang YZhang XZheng HZhang QYou JHuang ZChen YDove Medical PressarticleN-acetylcysteine (NAC)iron oxide nanoparticlesOxidative stressCardiomyocytesHypoxia-ReoxygenationMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 14, Pp 6103-6115 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	N-acetylcysteine (NAC) iron oxide nanoparticles Oxidative stress Cardiomyocytes Hypoxia-Reoxygenation Medicine (General) R5-920
spellingShingle	N-acetylcysteine (NAC) iron oxide nanoparticles Oxidative stress Cardiomyocytes Hypoxia-Reoxygenation Medicine (General) R5-920 Shen Y Gong S Li J Wang Y Zhang X Zheng H Zhang Q You J Huang Z Chen Y Co-loading antioxidant N-acetylcysteine attenuates cytotoxicity of iron oxide nanoparticles in hypoxia/reoxygenation cardiomyocytes
description	Yunli Shen,1,* Shiyu Gong,1,* Jiming Li,1,* Yunkai Wang,1 Xumin Zhang,1 Hao Zheng,1 Qi Zhang,1 Jieyun You,1 Zheyong Huang,2 Yihan Chen11Department of Cardiology, Shanghai East Hospital, Tongji University School of Medicine, Shanghai 200120, People’s Republic of China; 2Shanghai Institute of Cardiovascular Diseases, Zhongshan Hospital, Fudan University, Shanghai 200032, People’s Republic of China*These authors contributed equally to this workPurpose: Myocardial delivery of magnetic iron oxide nanoparticles (MNPs) might produce iron overload-induced myocardial injury, and the oxidative stress was regarded as the main mechanism. Therefore, we speculated antioxidant modification might be a reasonable strategy to mitigate the toxicity of MNPs.Methods and results: Antioxidant N-acetylcysteine (NAC) was loaded into magnetic mesoporous silica coated Fe3O4 nanoparticles. Neonatal rat hypoxia/reoxygenation (H/R) cardiomyocytes were incubated with nanoparticles for 24 hrs. NAC can effectively mitigate iron-induced oxidative injury of cardiomyocytes, evidenced by reduced production of MDA, 8-iso-PGF2α, and 8-OHDG and maintained concentrations of SOD, CAT, GSH-Px, and GSH in ELISA and biochemical tests; downregulated expression of CHOP, GRP78, p62, and LC3-II proteins in Western Blot, and less cardiomyocytes apoptosis in flow cytometric analysis.Conclusions: NAC modifying could suppress the toxic effects of Fe3O4 nanoparticles in H/R cardiomyocytes model in vitro, indicating a promising strategy to improve the safety of iron oxide nanoparticles.Keywords: N-acetylcysteine (NAC), iron oxide nanoparticles, oxidative stress, cardiomyocytes, hypoxia-reoxygenation
format	article
author	Shen Y Gong S Li J Wang Y Zhang X Zheng H Zhang Q You J Huang Z Chen Y
author_facet	Shen Y Gong S Li J Wang Y Zhang X Zheng H Zhang Q You J Huang Z Chen Y
author_sort	Shen Y
title	Co-loading antioxidant N-acetylcysteine attenuates cytotoxicity of iron oxide nanoparticles in hypoxia/reoxygenation cardiomyocytes
title_short	Co-loading antioxidant N-acetylcysteine attenuates cytotoxicity of iron oxide nanoparticles in hypoxia/reoxygenation cardiomyocytes
title_full	Co-loading antioxidant N-acetylcysteine attenuates cytotoxicity of iron oxide nanoparticles in hypoxia/reoxygenation cardiomyocytes
title_fullStr	Co-loading antioxidant N-acetylcysteine attenuates cytotoxicity of iron oxide nanoparticles in hypoxia/reoxygenation cardiomyocytes
title_full_unstemmed	Co-loading antioxidant N-acetylcysteine attenuates cytotoxicity of iron oxide nanoparticles in hypoxia/reoxygenation cardiomyocytes
title_sort	co-loading antioxidant n-acetylcysteine attenuates cytotoxicity of iron oxide nanoparticles in hypoxia/reoxygenation cardiomyocytes
publisher	Dove Medical Press
publishDate	2019
url	https://doaj.org/article/0d555e335bf2419fbd28b22741c3b203
work_keys_str_mv	AT sheny coloadingantioxidantnacetylcysteineattenuatescytotoxicityofironoxidenanoparticlesinhypoxiareoxygenationcardiomyocytes AT gongs coloadingantioxidantnacetylcysteineattenuatescytotoxicityofironoxidenanoparticlesinhypoxiareoxygenationcardiomyocytes AT lij coloadingantioxidantnacetylcysteineattenuatescytotoxicityofironoxidenanoparticlesinhypoxiareoxygenationcardiomyocytes AT wangy coloadingantioxidantnacetylcysteineattenuatescytotoxicityofironoxidenanoparticlesinhypoxiareoxygenationcardiomyocytes AT zhangx coloadingantioxidantnacetylcysteineattenuatescytotoxicityofironoxidenanoparticlesinhypoxiareoxygenationcardiomyocytes AT zhengh coloadingantioxidantnacetylcysteineattenuatescytotoxicityofironoxidenanoparticlesinhypoxiareoxygenationcardiomyocytes AT zhangq coloadingantioxidantnacetylcysteineattenuatescytotoxicityofironoxidenanoparticlesinhypoxiareoxygenationcardiomyocytes AT youj coloadingantioxidantnacetylcysteineattenuatescytotoxicityofironoxidenanoparticlesinhypoxiareoxygenationcardiomyocytes AT huangz coloadingantioxidantnacetylcysteineattenuatescytotoxicityofironoxidenanoparticlesinhypoxiareoxygenationcardiomyocytes AT cheny coloadingantioxidantnacetylcysteineattenuatescytotoxicityofironoxidenanoparticlesinhypoxiareoxygenationcardiomyocytes
_version_	1718398165654175744

Co-loading antioxidant N-acetylcysteine attenuates cytotoxicity of iron oxide nanoparticles in hypoxia/reoxygenation cardiomyocytes

Ejemplares similares