Chromoanagenesis from radiation-induced genome damage in Populus.

Chromoanagenesis is a genomic catastrophe that results in chromosomal shattering and reassembly. These extreme single chromosome events were first identified in cancer, and have since been observed in other systems, but have so far only been formally documented in plants in the context of haploid in...

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Autores principales:	Weier Guo, Luca Comai, Isabelle M Henry
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Genetics QH426-470
Acceso en línea:	https://doaj.org/article/0d5eaf75d5e84319806e2040a9d07e88
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spelling	oai:doaj.org-article:0d5eaf75d5e84319806e2040a9d07e882021-12-02T20:02:51ZChromoanagenesis from radiation-induced genome damage in Populus.1553-73901553-740410.1371/journal.pgen.1009735https://doaj.org/article/0d5eaf75d5e84319806e2040a9d07e882021-08-01T00:00:00Zhttps://doi.org/10.1371/journal.pgen.1009735https://doaj.org/toc/1553-7390https://doaj.org/toc/1553-7404Chromoanagenesis is a genomic catastrophe that results in chromosomal shattering and reassembly. These extreme single chromosome events were first identified in cancer, and have since been observed in other systems, but have so far only been formally documented in plants in the context of haploid induction crosses. The frequency, origins, consequences, and evolutionary impact of such major chromosomal remodeling in other situations remain obscure. Here, we demonstrate the occurrence of chromoanagenesis in poplar (Populus sp.) trees produced from gamma-irradiated pollen. Specifically, in this population of siblings carrying indel mutations, two individuals exhibited highly frequent copy number variation (CNV) clustered on a single chromosome, one of the hallmarks of chromoanagenesis. Using short-read sequencing, we confirmed the presence of clustered segmental rearrangement. Independently, we identified and validated novel DNA junctions and confirmed that they were clustered and corresponded to these rearrangements. Our reconstruction of the novel sequences suggests that the chromosomal segments have reorganized randomly to produce a novel rearranged chromosome but that two different mechanisms might be at play. Our results indicate that gamma irradiation can trigger chromoanagenesis, suggesting that this may also occur when natural or induced mutagens cause DNA breaks. We further demonstrate that such events can be tolerated in poplar, and even replicated clonally, providing an attractive system for more in-depth investigations of their consequences.Weier GuoLuca ComaiIsabelle M HenryPublic Library of Science (PLoS)articleGeneticsQH426-470ENPLoS Genetics, Vol 17, Iss 8, p e1009735 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Genetics QH426-470
spellingShingle	Genetics QH426-470 Weier Guo Luca Comai Isabelle M Henry Chromoanagenesis from radiation-induced genome damage in Populus.
description	Chromoanagenesis is a genomic catastrophe that results in chromosomal shattering and reassembly. These extreme single chromosome events were first identified in cancer, and have since been observed in other systems, but have so far only been formally documented in plants in the context of haploid induction crosses. The frequency, origins, consequences, and evolutionary impact of such major chromosomal remodeling in other situations remain obscure. Here, we demonstrate the occurrence of chromoanagenesis in poplar (Populus sp.) trees produced from gamma-irradiated pollen. Specifically, in this population of siblings carrying indel mutations, two individuals exhibited highly frequent copy number variation (CNV) clustered on a single chromosome, one of the hallmarks of chromoanagenesis. Using short-read sequencing, we confirmed the presence of clustered segmental rearrangement. Independently, we identified and validated novel DNA junctions and confirmed that they were clustered and corresponded to these rearrangements. Our reconstruction of the novel sequences suggests that the chromosomal segments have reorganized randomly to produce a novel rearranged chromosome but that two different mechanisms might be at play. Our results indicate that gamma irradiation can trigger chromoanagenesis, suggesting that this may also occur when natural or induced mutagens cause DNA breaks. We further demonstrate that such events can be tolerated in poplar, and even replicated clonally, providing an attractive system for more in-depth investigations of their consequences.
format	article
author	Weier Guo Luca Comai Isabelle M Henry
author_facet	Weier Guo Luca Comai Isabelle M Henry
author_sort	Weier Guo
title	Chromoanagenesis from radiation-induced genome damage in Populus.
title_short	Chromoanagenesis from radiation-induced genome damage in Populus.
title_full	Chromoanagenesis from radiation-induced genome damage in Populus.
title_fullStr	Chromoanagenesis from radiation-induced genome damage in Populus.
title_full_unstemmed	Chromoanagenesis from radiation-induced genome damage in Populus.
title_sort	chromoanagenesis from radiation-induced genome damage in populus.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/0d5eaf75d5e84319806e2040a9d07e88
work_keys_str_mv	AT weierguo chromoanagenesisfromradiationinducedgenomedamageinpopulus AT lucacomai chromoanagenesisfromradiationinducedgenomedamageinpopulus AT isabellemhenry chromoanagenesisfromradiationinducedgenomedamageinpopulus
_version_	1718375643426586624

Chromoanagenesis from radiation-induced genome damage in Populus.

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