mTOR inhibitors potentially reduce TGF-β2-induced fibrogenic changes in trabecular meshwork cells

Abstract We examined the effects of mTOR inhibitors on the fibrotic response induced by transforming growth factor-beta2 (TGF-β2) in cultured human trabecular meshwork (hTM) cells. TGF-β2-induced expression of fibronectin, collagen type I, alpha 1 chain (COL1A1), and alpha-smooth muscle actin (αSMA)...

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Autores principales:	Nozomi Igarashi, Megumi Honjo, Makoto Aihara
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/0d72df1cb3494545b07a52c213b76667
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spelling	oai:doaj.org-article:0d72df1cb3494545b07a52c213b766672021-12-02T15:39:50ZmTOR inhibitors potentially reduce TGF-β2-induced fibrogenic changes in trabecular meshwork cells10.1038/s41598-021-93580-32045-2322https://doaj.org/article/0d72df1cb3494545b07a52c213b766672021-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-93580-3https://doaj.org/toc/2045-2322Abstract We examined the effects of mTOR inhibitors on the fibrotic response induced by transforming growth factor-beta2 (TGF-β2) in cultured human trabecular meshwork (hTM) cells. TGF-β2-induced expression of fibronectin, collagen type I, alpha 1 chain (COL1A1), and alpha-smooth muscle actin (αSMA) in hTM cells was examined in the presence or absence of mTOR inhibitors using quantitative real-time polymerase chain reaction, Western blotting, and immunohistochemistry. The migration rates of hTM cells were examined in the presence of TGF-β2 with or without mTOR inhibitors. An in vitro study showed that the expression of fibronectin, COL1A1, and αSMA was upregulated by TGF-β2 treatment of hTM cells; such upregulation was significantly suppressed by mTOR inhibitors. The inhibitors significantly reduced the migration rate of TGF-β2-stimulated hTM cells. mTOR inhibitors may usefully reduce the fibrotic response of hTM cells and we may have to explore if it is also effective in in vivo model.Nozomi IgarashiMegumi HonjoMakoto AiharaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-10 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Nozomi Igarashi Megumi Honjo Makoto Aihara mTOR inhibitors potentially reduce TGF-β2-induced fibrogenic changes in trabecular meshwork cells
description	Abstract We examined the effects of mTOR inhibitors on the fibrotic response induced by transforming growth factor-beta2 (TGF-β2) in cultured human trabecular meshwork (hTM) cells. TGF-β2-induced expression of fibronectin, collagen type I, alpha 1 chain (COL1A1), and alpha-smooth muscle actin (αSMA) in hTM cells was examined in the presence or absence of mTOR inhibitors using quantitative real-time polymerase chain reaction, Western blotting, and immunohistochemistry. The migration rates of hTM cells were examined in the presence of TGF-β2 with or without mTOR inhibitors. An in vitro study showed that the expression of fibronectin, COL1A1, and αSMA was upregulated by TGF-β2 treatment of hTM cells; such upregulation was significantly suppressed by mTOR inhibitors. The inhibitors significantly reduced the migration rate of TGF-β2-stimulated hTM cells. mTOR inhibitors may usefully reduce the fibrotic response of hTM cells and we may have to explore if it is also effective in in vivo model.
format	article
author	Nozomi Igarashi Megumi Honjo Makoto Aihara
author_facet	Nozomi Igarashi Megumi Honjo Makoto Aihara
author_sort	Nozomi Igarashi
title	mTOR inhibitors potentially reduce TGF-β2-induced fibrogenic changes in trabecular meshwork cells
title_short	mTOR inhibitors potentially reduce TGF-β2-induced fibrogenic changes in trabecular meshwork cells
title_full	mTOR inhibitors potentially reduce TGF-β2-induced fibrogenic changes in trabecular meshwork cells
title_fullStr	mTOR inhibitors potentially reduce TGF-β2-induced fibrogenic changes in trabecular meshwork cells
title_full_unstemmed	mTOR inhibitors potentially reduce TGF-β2-induced fibrogenic changes in trabecular meshwork cells
title_sort	mtor inhibitors potentially reduce tgf-β2-induced fibrogenic changes in trabecular meshwork cells
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/0d72df1cb3494545b07a52c213b76667
work_keys_str_mv	AT nozomiigarashi mtorinhibitorspotentiallyreducetgfb2inducedfibrogenicchangesintrabecularmeshworkcells AT megumihonjo mtorinhibitorspotentiallyreducetgfb2inducedfibrogenicchangesintrabecularmeshworkcells AT makotoaihara mtorinhibitorspotentiallyreducetgfb2inducedfibrogenicchangesintrabecularmeshworkcells
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mTOR inhibitors potentially reduce TGF-β2-induced fibrogenic changes in trabecular meshwork cells

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