Evaluation of the Anti-Shigellosis Activity of Dietary Isothiocyanates in <i>Galleria mellonella</i> Larvae

Cruciferous vegetables, widely present in daily diets, are a rich source of organosulfur compounds with proven health benefits, especially chemopreventive or antioxidative effects. Isothiocyanate derivatives (ITCs) exhibit a broad spectrum of biological and pharmacological activity and recently, the...

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Autores principales:	Dariusz Nowicki, Klaudyna Krause, Monika Karczewska, Agnieszka Szalewska-Pałasz
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	<i>Shigella dysenteriae</i> isothiocyanates sulforaphane phenethyl isothiocyanate stringent response <i>Galleria mellonella</i> Nutrition. Foods and food supply TX341-641
Acceso en línea:	https://doaj.org/article/0d75db2fc9ce4d69900c685c3530a498
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spelling	oai:doaj.org-article:0d75db2fc9ce4d69900c685c3530a4982021-11-25T18:35:40ZEvaluation of the Anti-Shigellosis Activity of Dietary Isothiocyanates in <i>Galleria mellonella</i> Larvae10.3390/nu131139672072-6643https://doaj.org/article/0d75db2fc9ce4d69900c685c3530a4982021-11-01T00:00:00Zhttps://www.mdpi.com/2072-6643/13/11/3967https://doaj.org/toc/2072-6643Cruciferous vegetables, widely present in daily diets, are a rich source of organosulfur compounds with proven health benefits, especially chemopreventive or antioxidative effects. Isothiocyanate derivatives (ITCs) exhibit a broad spectrum of biological and pharmacological activity and recently, their antibacterial properties have been of particular importance. Here, we have focused on the anti-shigellosis activity of sulforaphane (SFN) and phenethyl ITC (PEITC). The genus <i>Shigella</i> causes gastroenteritis in humans, which constitutes a threat to public health. Production of a potent Stx toxin by <i>S. dysenteriae</i> type 1 results not only in more severe symptoms but also in serious sequela, including the hemolytic uremic syndrome. Here, we present evidence that two aliphatic and aromatic ITCs derivatives, SFN and PEITC, have an effective antibacterial potency against <i>S. dysenteriae,</i> also negatively regulating the <i>stx</i> gene expression. The molecular mechanism of this effect involves induction of the global stress-induced stringent response. ITCs also inhibit bacterial virulence against the Vero and HeLa cells. We present evidence for the therapeutic effect of sulforaphane and phenethyl ITC against a <i>S. dysenteriae</i> infection in the <i>Galleria mellonella</i> larvae model. Thus, our results indicate that isothiocyanates can be effectively used to combat dangerous bacterial infections.Dariusz NowickiKlaudyna KrauseMonika KarczewskaAgnieszka Szalewska-PałaszMDPI AGarticle<i>Shigella dysenteriae</i>isothiocyanatessulforaphanephenethyl isothiocyanatestringent response<i>Galleria mellonella</i>Nutrition. Foods and food supplyTX341-641ENNutrients, Vol 13, Iss 3967, p 3967 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	<i>Shigella dysenteriae</i> isothiocyanates sulforaphane phenethyl isothiocyanate stringent response <i>Galleria mellonella</i> Nutrition. Foods and food supply TX341-641
spellingShingle	<i>Shigella dysenteriae</i> isothiocyanates sulforaphane phenethyl isothiocyanate stringent response <i>Galleria mellonella</i> Nutrition. Foods and food supply TX341-641 Dariusz Nowicki Klaudyna Krause Monika Karczewska Agnieszka Szalewska-Pałasz Evaluation of the Anti-Shigellosis Activity of Dietary Isothiocyanates in <i>Galleria mellonella</i> Larvae
description	Cruciferous vegetables, widely present in daily diets, are a rich source of organosulfur compounds with proven health benefits, especially chemopreventive or antioxidative effects. Isothiocyanate derivatives (ITCs) exhibit a broad spectrum of biological and pharmacological activity and recently, their antibacterial properties have been of particular importance. Here, we have focused on the anti-shigellosis activity of sulforaphane (SFN) and phenethyl ITC (PEITC). The genus <i>Shigella</i> causes gastroenteritis in humans, which constitutes a threat to public health. Production of a potent Stx toxin by <i>S. dysenteriae</i> type 1 results not only in more severe symptoms but also in serious sequela, including the hemolytic uremic syndrome. Here, we present evidence that two aliphatic and aromatic ITCs derivatives, SFN and PEITC, have an effective antibacterial potency against <i>S. dysenteriae,</i> also negatively regulating the <i>stx</i> gene expression. The molecular mechanism of this effect involves induction of the global stress-induced stringent response. ITCs also inhibit bacterial virulence against the Vero and HeLa cells. We present evidence for the therapeutic effect of sulforaphane and phenethyl ITC against a <i>S. dysenteriae</i> infection in the <i>Galleria mellonella</i> larvae model. Thus, our results indicate that isothiocyanates can be effectively used to combat dangerous bacterial infections.
format	article
author	Dariusz Nowicki Klaudyna Krause Monika Karczewska Agnieszka Szalewska-Pałasz
author_facet	Dariusz Nowicki Klaudyna Krause Monika Karczewska Agnieszka Szalewska-Pałasz
author_sort	Dariusz Nowicki
title	Evaluation of the Anti-Shigellosis Activity of Dietary Isothiocyanates in <i>Galleria mellonella</i> Larvae
title_short	Evaluation of the Anti-Shigellosis Activity of Dietary Isothiocyanates in <i>Galleria mellonella</i> Larvae
title_full	Evaluation of the Anti-Shigellosis Activity of Dietary Isothiocyanates in <i>Galleria mellonella</i> Larvae
title_fullStr	Evaluation of the Anti-Shigellosis Activity of Dietary Isothiocyanates in <i>Galleria mellonella</i> Larvae
title_full_unstemmed	Evaluation of the Anti-Shigellosis Activity of Dietary Isothiocyanates in <i>Galleria mellonella</i> Larvae
title_sort	evaluation of the anti-shigellosis activity of dietary isothiocyanates in <i>galleria mellonella</i> larvae
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/0d75db2fc9ce4d69900c685c3530a498
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Evaluation of the Anti-Shigellosis Activity of Dietary Isothiocyanates in <i>Galleria mellonella</i> Larvae

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