Chronic pathophysiological changes in the normal brain parenchyma caused by radiotherapy accelerate glioma progression

Abstract Radiation therapy is one of standard treatment for malignant glioma after surgery. The microenvironment after irradiation is considered not to be suitable for the survival of tumor cells (tumor bed effect). This study investigated whether the effect of changes in the microenvironment of par...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Yuichiro Tsuji, Naosuke Nonoguchi, Daisuke Okuzaki, Yusuke Wada, Daisuke Motooka, Yuki Hirota, Taichiro Toho, Nobuhiko Yoshikawa, Motomasa Furuse, Shinji Kawabata, Shin-Ichi Miyatake, Hiroyuki Nakamura, Ryohei Yamamoto, Shota Nakamura, Toshihiko Kuroiwa, Masahiko Wanibuchi
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/0d7b40efdd7d417caacb63654302e46c
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:0d7b40efdd7d417caacb63654302e46c
record_format	dspace
spelling	oai:doaj.org-article:0d7b40efdd7d417caacb63654302e46c2021-11-14T12:22:41ZChronic pathophysiological changes in the normal brain parenchyma caused by radiotherapy accelerate glioma progression10.1038/s41598-021-01475-02045-2322https://doaj.org/article/0d7b40efdd7d417caacb63654302e46c2021-11-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-01475-0https://doaj.org/toc/2045-2322Abstract Radiation therapy is one of standard treatment for malignant glioma after surgery. The microenvironment after irradiation is considered not to be suitable for the survival of tumor cells (tumor bed effect). This study investigated whether the effect of changes in the microenvironment of parenchymal brain tissue caused by radiotherapy affect the recurrence and progression of glioma. 65-Gy irradiation had been applied to the right hemisphere of Fisher rats. After 3 months from irradiation, we extracted RNA and protein from the irradiated rat brain. To study effects of proteins extracted from the brains, we performed WST-8 assay and tube formation assay in vitro. Cytokine production were investigated for qPCR. Additionally, we transplanted glioma cell into the irradiated and sham animals and the median survival time of F98 transplanted rats was also examined in vivo. Immunohistochemical analyses and invasiveness of implanted tumor were evaluated. X-ray irradiation promoted the secretion of cytokines such as CXCL12, VEGF-A, TGF-β1 and TNFα from the irradiated brain. Proteins extracted from the irradiated brain promoted the proliferation and angiogenic activity of F98 glioma cells. Glioma cells implanted in the irradiated brains showed significantly high proliferation, angiogenesis and invasive ability, and the post-irradiation F98 tumor-implanted rats showed a shorter median survival time compared to the Sham-irradiation group. The current study suggests that the microenvironment around the brain tissue in the chronic phase after exposure to X-ray radiation becomes suitable for glioma cell growth and invasion.Yuichiro TsujiNaosuke NonoguchiDaisuke OkuzakiYusuke WadaDaisuke MotookaYuki HirotaTaichiro TohoNobuhiko YoshikawaMotomasa FuruseShinji KawabataShin-Ichi MiyatakeHiroyuki NakamuraRyohei YamamotoShota NakamuraToshihiko KuroiwaMasahiko WanibuchiNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-15 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Yuichiro Tsuji Naosuke Nonoguchi Daisuke Okuzaki Yusuke Wada Daisuke Motooka Yuki Hirota Taichiro Toho Nobuhiko Yoshikawa Motomasa Furuse Shinji Kawabata Shin-Ichi Miyatake Hiroyuki Nakamura Ryohei Yamamoto Shota Nakamura Toshihiko Kuroiwa Masahiko Wanibuchi Chronic pathophysiological changes in the normal brain parenchyma caused by radiotherapy accelerate glioma progression
description	Abstract Radiation therapy is one of standard treatment for malignant glioma after surgery. The microenvironment after irradiation is considered not to be suitable for the survival of tumor cells (tumor bed effect). This study investigated whether the effect of changes in the microenvironment of parenchymal brain tissue caused by radiotherapy affect the recurrence and progression of glioma. 65-Gy irradiation had been applied to the right hemisphere of Fisher rats. After 3 months from irradiation, we extracted RNA and protein from the irradiated rat brain. To study effects of proteins extracted from the brains, we performed WST-8 assay and tube formation assay in vitro. Cytokine production were investigated for qPCR. Additionally, we transplanted glioma cell into the irradiated and sham animals and the median survival time of F98 transplanted rats was also examined in vivo. Immunohistochemical analyses and invasiveness of implanted tumor were evaluated. X-ray irradiation promoted the secretion of cytokines such as CXCL12, VEGF-A, TGF-β1 and TNFα from the irradiated brain. Proteins extracted from the irradiated brain promoted the proliferation and angiogenic activity of F98 glioma cells. Glioma cells implanted in the irradiated brains showed significantly high proliferation, angiogenesis and invasive ability, and the post-irradiation F98 tumor-implanted rats showed a shorter median survival time compared to the Sham-irradiation group. The current study suggests that the microenvironment around the brain tissue in the chronic phase after exposure to X-ray radiation becomes suitable for glioma cell growth and invasion.
format	article
author	Yuichiro Tsuji Naosuke Nonoguchi Daisuke Okuzaki Yusuke Wada Daisuke Motooka Yuki Hirota Taichiro Toho Nobuhiko Yoshikawa Motomasa Furuse Shinji Kawabata Shin-Ichi Miyatake Hiroyuki Nakamura Ryohei Yamamoto Shota Nakamura Toshihiko Kuroiwa Masahiko Wanibuchi
author_facet	Yuichiro Tsuji Naosuke Nonoguchi Daisuke Okuzaki Yusuke Wada Daisuke Motooka Yuki Hirota Taichiro Toho Nobuhiko Yoshikawa Motomasa Furuse Shinji Kawabata Shin-Ichi Miyatake Hiroyuki Nakamura Ryohei Yamamoto Shota Nakamura Toshihiko Kuroiwa Masahiko Wanibuchi
author_sort	Yuichiro Tsuji
title	Chronic pathophysiological changes in the normal brain parenchyma caused by radiotherapy accelerate glioma progression
title_short	Chronic pathophysiological changes in the normal brain parenchyma caused by radiotherapy accelerate glioma progression
title_full	Chronic pathophysiological changes in the normal brain parenchyma caused by radiotherapy accelerate glioma progression
title_fullStr	Chronic pathophysiological changes in the normal brain parenchyma caused by radiotherapy accelerate glioma progression
title_full_unstemmed	Chronic pathophysiological changes in the normal brain parenchyma caused by radiotherapy accelerate glioma progression
title_sort	chronic pathophysiological changes in the normal brain parenchyma caused by radiotherapy accelerate glioma progression
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/0d7b40efdd7d417caacb63654302e46c
work_keys_str_mv	AT yuichirotsuji chronicpathophysiologicalchangesinthenormalbrainparenchymacausedbyradiotherapyaccelerategliomaprogression AT naosukenonoguchi chronicpathophysiologicalchangesinthenormalbrainparenchymacausedbyradiotherapyaccelerategliomaprogression AT daisukeokuzaki chronicpathophysiologicalchangesinthenormalbrainparenchymacausedbyradiotherapyaccelerategliomaprogression AT yusukewada chronicpathophysiologicalchangesinthenormalbrainparenchymacausedbyradiotherapyaccelerategliomaprogression AT daisukemotooka chronicpathophysiologicalchangesinthenormalbrainparenchymacausedbyradiotherapyaccelerategliomaprogression AT yukihirota chronicpathophysiologicalchangesinthenormalbrainparenchymacausedbyradiotherapyaccelerategliomaprogression AT taichirotoho chronicpathophysiologicalchangesinthenormalbrainparenchymacausedbyradiotherapyaccelerategliomaprogression AT nobuhikoyoshikawa chronicpathophysiologicalchangesinthenormalbrainparenchymacausedbyradiotherapyaccelerategliomaprogression AT motomasafuruse chronicpathophysiologicalchangesinthenormalbrainparenchymacausedbyradiotherapyaccelerategliomaprogression AT shinjikawabata chronicpathophysiologicalchangesinthenormalbrainparenchymacausedbyradiotherapyaccelerategliomaprogression AT shinichimiyatake chronicpathophysiologicalchangesinthenormalbrainparenchymacausedbyradiotherapyaccelerategliomaprogression AT hiroyukinakamura chronicpathophysiologicalchangesinthenormalbrainparenchymacausedbyradiotherapyaccelerategliomaprogression AT ryoheiyamamoto chronicpathophysiologicalchangesinthenormalbrainparenchymacausedbyradiotherapyaccelerategliomaprogression AT shotanakamura chronicpathophysiologicalchangesinthenormalbrainparenchymacausedbyradiotherapyaccelerategliomaprogression AT toshihikokuroiwa chronicpathophysiologicalchangesinthenormalbrainparenchymacausedbyradiotherapyaccelerategliomaprogression AT masahikowanibuchi chronicpathophysiologicalchangesinthenormalbrainparenchymacausedbyradiotherapyaccelerategliomaprogression
_version_	1718429211331395584

Chronic pathophysiological changes in the normal brain parenchyma caused by radiotherapy accelerate glioma progression

Ejemplares similares