Fetal mesenchymal stromal cells differentiating towards chondrocytes acquire a gene expression profile resembling human growth plate cartilage.

We used human fetal bone marrow-derived mesenchymal stromal cells (hfMSCs) differentiating towards chondrocytes as an alternative model for the human growth plate (GP). Our aims were to study gene expression patterns associated with chondrogenic differentiation to assess whether chondrocytes derived...

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Autores principales:	Sandy A van Gool, Joyce A M Emons, Jeroen C H Leijten, Eva Decker, Carsten Sticht, Johannes C van Houwelingen, Jelle J Goeman, Carin Kleijburg, Sicco A Scherjon, Norbert Gretz, Jan Maarten Wit, Gudrun Rappold, Janine N Post, Marcel Karperien
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/0d7f3ea473844d028838b15a55294cfd
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spelling	oai:doaj.org-article:0d7f3ea473844d028838b15a55294cfd2021-11-18T08:10:03ZFetal mesenchymal stromal cells differentiating towards chondrocytes acquire a gene expression profile resembling human growth plate cartilage.1932-620310.1371/journal.pone.0044561https://doaj.org/article/0d7f3ea473844d028838b15a55294cfd2012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23144774/?tool=EBIhttps://doaj.org/toc/1932-6203We used human fetal bone marrow-derived mesenchymal stromal cells (hfMSCs) differentiating towards chondrocytes as an alternative model for the human growth plate (GP). Our aims were to study gene expression patterns associated with chondrogenic differentiation to assess whether chondrocytes derived from hfMSCs are a suitable model for studying the development and maturation of the GP. hfMSCs efficiently formed hyaline cartilage in a pellet culture in the presence of TGFβ3 and BMP6. Microarray and principal component analysis were applied to study gene expression profiles during chondrogenic differentiation. A set of 232 genes was found to correlate with in vitro cartilage formation. Several identified genes are known to be involved in cartilage formation and validate the robustness of the differentiating hfMSC model. KEGG pathway analysis using the 232 genes revealed 9 significant signaling pathways correlated with cartilage formation. To determine the progression of growth plate cartilage formation, we compared the gene expression profile of differentiating hfMSCs with previously established expression profiles of epiphyseal GP cartilage. As differentiation towards chondrocytes proceeds, hfMSCs gradually obtain a gene expression profile resembling epiphyseal GP cartilage. We visualized the differences in gene expression profiles as protein interaction clusters and identified many protein clusters that are activated during the early chondrogenic differentiation of hfMSCs showing the potential of this system to study GP development.Sandy A van GoolJoyce A M EmonsJeroen C H LeijtenEva DeckerCarsten StichtJohannes C van HouwelingenJelle J GoemanCarin KleijburgSicco A ScherjonNorbert GretzJan Maarten WitGudrun RappoldJanine N PostMarcel KarperienPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 11, p e44561 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Sandy A van Gool Joyce A M Emons Jeroen C H Leijten Eva Decker Carsten Sticht Johannes C van Houwelingen Jelle J Goeman Carin Kleijburg Sicco A Scherjon Norbert Gretz Jan Maarten Wit Gudrun Rappold Janine N Post Marcel Karperien Fetal mesenchymal stromal cells differentiating towards chondrocytes acquire a gene expression profile resembling human growth plate cartilage.
description	We used human fetal bone marrow-derived mesenchymal stromal cells (hfMSCs) differentiating towards chondrocytes as an alternative model for the human growth plate (GP). Our aims were to study gene expression patterns associated with chondrogenic differentiation to assess whether chondrocytes derived from hfMSCs are a suitable model for studying the development and maturation of the GP. hfMSCs efficiently formed hyaline cartilage in a pellet culture in the presence of TGFβ3 and BMP6. Microarray and principal component analysis were applied to study gene expression profiles during chondrogenic differentiation. A set of 232 genes was found to correlate with in vitro cartilage formation. Several identified genes are known to be involved in cartilage formation and validate the robustness of the differentiating hfMSC model. KEGG pathway analysis using the 232 genes revealed 9 significant signaling pathways correlated with cartilage formation. To determine the progression of growth plate cartilage formation, we compared the gene expression profile of differentiating hfMSCs with previously established expression profiles of epiphyseal GP cartilage. As differentiation towards chondrocytes proceeds, hfMSCs gradually obtain a gene expression profile resembling epiphyseal GP cartilage. We visualized the differences in gene expression profiles as protein interaction clusters and identified many protein clusters that are activated during the early chondrogenic differentiation of hfMSCs showing the potential of this system to study GP development.
format	article
author	Sandy A van Gool Joyce A M Emons Jeroen C H Leijten Eva Decker Carsten Sticht Johannes C van Houwelingen Jelle J Goeman Carin Kleijburg Sicco A Scherjon Norbert Gretz Jan Maarten Wit Gudrun Rappold Janine N Post Marcel Karperien
author_facet	Sandy A van Gool Joyce A M Emons Jeroen C H Leijten Eva Decker Carsten Sticht Johannes C van Houwelingen Jelle J Goeman Carin Kleijburg Sicco A Scherjon Norbert Gretz Jan Maarten Wit Gudrun Rappold Janine N Post Marcel Karperien
author_sort	Sandy A van Gool
title	Fetal mesenchymal stromal cells differentiating towards chondrocytes acquire a gene expression profile resembling human growth plate cartilage.
title_short	Fetal mesenchymal stromal cells differentiating towards chondrocytes acquire a gene expression profile resembling human growth plate cartilage.
title_full	Fetal mesenchymal stromal cells differentiating towards chondrocytes acquire a gene expression profile resembling human growth plate cartilage.
title_fullStr	Fetal mesenchymal stromal cells differentiating towards chondrocytes acquire a gene expression profile resembling human growth plate cartilage.
title_full_unstemmed	Fetal mesenchymal stromal cells differentiating towards chondrocytes acquire a gene expression profile resembling human growth plate cartilage.
title_sort	fetal mesenchymal stromal cells differentiating towards chondrocytes acquire a gene expression profile resembling human growth plate cartilage.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/0d7f3ea473844d028838b15a55294cfd
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Fetal mesenchymal stromal cells differentiating towards chondrocytes acquire a gene expression profile resembling human growth plate cartilage.

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