Association of microRNA-34a rs2666433 (A/G) Variant with Systemic Lupus Erythematosus in Female Patients: A Case-Control Study

Several microRNAs (miRNAs) are associated with autoimmune disease susceptibility and phenotype, including systemic lupus erythematosus (SLE). We aimed to explore for the first time the role of the miRNA-34a gene (MIR34A) rs2666433A > G variant in SLE risk and severity. A total of 163 adult patien...

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Autores principales:	Nesreen M. Ismail, Eman A. Toraih, Mai H. S. Mohammad, Eida M. Alshammari, Manal S. Fawzy
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	MIR34A lupus nephritis single nucleotide polymorphism SLE Medicine R
Acceso en línea:	https://doaj.org/article/0d80cbd5e93d46408a9dac27a1ab73c8
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spelling	oai:doaj.org-article:0d80cbd5e93d46408a9dac27a1ab73c82021-11-11T17:43:01ZAssociation of microRNA-34a rs2666433 (A/G) Variant with Systemic Lupus Erythematosus in Female Patients: A Case-Control Study10.3390/jcm102150952077-0383https://doaj.org/article/0d80cbd5e93d46408a9dac27a1ab73c82021-10-01T00:00:00Zhttps://www.mdpi.com/2077-0383/10/21/5095https://doaj.org/toc/2077-0383Several microRNAs (miRNAs) are associated with autoimmune disease susceptibility and phenotype, including systemic lupus erythematosus (SLE). We aimed to explore for the first time the role of the miRNA-34a gene (MIR34A) rs2666433A > G variant in SLE risk and severity. A total of 163 adult patients with SLE and matched controls were recruited. Real-Time allelic discrimination PCR was applied for genotyping. Correlation with disease activity and clinic-laboratory data was done. The rs2666433 variant conferred protection against SLE development under heterozygous [A/G vs. G/G; OR = 0.57, 95%CI = 0.34–0.95], homozygous [A/A vs. G/G; OR = 0.52, 95%CI = 0.29–0.94], dominant [A/G + A/A vs. GG; OR = 0.55, 95%CI = 0.35–0.88], and log-additive [OR = 0.71, 95%CI = 0.53–0.95] models. Data stratification by sex revealed a significant association with SLE development in female participants under heterozygous/homozygous models (<i>p</i>-interaction = 0.004). There was no clear demarcation between SLE patients carrying different genotypes regarding the disease activity index or patients stratified according to lupus nephritis. Enrichment analysis confirmed the implication of MIR34A in the SLE pathway by targeting several genes related to SLE etiopathology. In conclusion, although the MIR34A rs2666433 variant conferred protection against developing SLE disease in the study population, it showed no association with disease activity. Replication studies in other populations are warranted.Nesreen M. IsmailEman A. ToraihMai H. S. MohammadEida M. AlshammariManal S. FawzyMDPI AGarticleMIR34Alupus nephritissingle nucleotide polymorphismSLEMedicineRENJournal of Clinical Medicine, Vol 10, Iss 5095, p 5095 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	MIR34A lupus nephritis single nucleotide polymorphism SLE Medicine R
spellingShingle	MIR34A lupus nephritis single nucleotide polymorphism SLE Medicine R Nesreen M. Ismail Eman A. Toraih Mai H. S. Mohammad Eida M. Alshammari Manal S. Fawzy Association of microRNA-34a rs2666433 (A/G) Variant with Systemic Lupus Erythematosus in Female Patients: A Case-Control Study
description	Several microRNAs (miRNAs) are associated with autoimmune disease susceptibility and phenotype, including systemic lupus erythematosus (SLE). We aimed to explore for the first time the role of the miRNA-34a gene (MIR34A) rs2666433A > G variant in SLE risk and severity. A total of 163 adult patients with SLE and matched controls were recruited. Real-Time allelic discrimination PCR was applied for genotyping. Correlation with disease activity and clinic-laboratory data was done. The rs2666433 variant conferred protection against SLE development under heterozygous [A/G vs. G/G; OR = 0.57, 95%CI = 0.34–0.95], homozygous [A/A vs. G/G; OR = 0.52, 95%CI = 0.29–0.94], dominant [A/G + A/A vs. GG; OR = 0.55, 95%CI = 0.35–0.88], and log-additive [OR = 0.71, 95%CI = 0.53–0.95] models. Data stratification by sex revealed a significant association with SLE development in female participants under heterozygous/homozygous models (<i>p</i>-interaction = 0.004). There was no clear demarcation between SLE patients carrying different genotypes regarding the disease activity index or patients stratified according to lupus nephritis. Enrichment analysis confirmed the implication of MIR34A in the SLE pathway by targeting several genes related to SLE etiopathology. In conclusion, although the MIR34A rs2666433 variant conferred protection against developing SLE disease in the study population, it showed no association with disease activity. Replication studies in other populations are warranted.
format	article
author	Nesreen M. Ismail Eman A. Toraih Mai H. S. Mohammad Eida M. Alshammari Manal S. Fawzy
author_facet	Nesreen M. Ismail Eman A. Toraih Mai H. S. Mohammad Eida M. Alshammari Manal S. Fawzy
author_sort	Nesreen M. Ismail
title	Association of microRNA-34a rs2666433 (A/G) Variant with Systemic Lupus Erythematosus in Female Patients: A Case-Control Study
title_short	Association of microRNA-34a rs2666433 (A/G) Variant with Systemic Lupus Erythematosus in Female Patients: A Case-Control Study
title_full	Association of microRNA-34a rs2666433 (A/G) Variant with Systemic Lupus Erythematosus in Female Patients: A Case-Control Study
title_fullStr	Association of microRNA-34a rs2666433 (A/G) Variant with Systemic Lupus Erythematosus in Female Patients: A Case-Control Study
title_full_unstemmed	Association of microRNA-34a rs2666433 (A/G) Variant with Systemic Lupus Erythematosus in Female Patients: A Case-Control Study
title_sort	association of microrna-34a rs2666433 (a/g) variant with systemic lupus erythematosus in female patients: a case-control study
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/0d80cbd5e93d46408a9dac27a1ab73c8
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Association of microRNA-34a rs2666433 (A/G) Variant with Systemic Lupus Erythematosus in Female Patients: A Case-Control Study

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