<i>CaWRKY30</i> Positively Regulates Pepper Immunity by Targeting <i>CaWRKY40</i> against <i>Ralstonia solanacearum</i> Inoculation through Modulating Defense-Related Genes
The WRKY transcription factors (TFs) network is composed of WRKY TFs’ subset, which performs a critical role in immunity regulation of plants. However, functions of WRKY TFs’ network remain unclear, particularly in non-model plants such as pepper (<i>Capsicum annuum</i> L.). This study f...
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| Autores principales: | , , , , , , , , , , |
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| Formato: | article |
| Lenguaje: | EN |
| Publicado: |
MDPI AG
2021
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| Materias: | |
| Acceso en línea: | https://doaj.org/article/0d8475a926ba41d187bb8344b63d57da |
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| Sumario: | The WRKY transcription factors (TFs) network is composed of WRKY TFs’ subset, which performs a critical role in immunity regulation of plants. However, functions of WRKY TFs’ network remain unclear, particularly in non-model plants such as pepper (<i>Capsicum annuum</i> L.). This study functionally characterized CaWRKY30—a member of group III Pepper WRKY protein—for immunity of pepper against <i>Ralstonia solanacearum</i> infection. The <i>CaWRKY30</i> was detected in nucleus, and its transcriptional expression levels were significantly upregulated by <i>R. solanacearum</i> inoculation (RSI), and foliar application ethylene (ET), abscisic acid (ABA), and salicylic acid (SA). Virus induced gene silencing (VIGS) of <i>CaWRKY30</i> amplified pepper’s vulnerability to RSI. Additionally, the silencing of <i>CaWRKY30</i> by VIGS compromised HR-like cell death triggered by RSI and downregulated defense-associated marker genes, like <i>CaPR1</i>, <i>CaNPR1</i>, <i>CaDEF1</i>, <i>CaABR1</i>, <i>CaHIR1</i>, and <i>CaWRKY40</i>. Conversely, transient over-expression of <i>CaWRKY30</i> in pepper leaves instigated HR-like cell death and upregulated defense-related maker genes. Furthermore, transient over-expression of <i>CaWRKY30</i> upregulated transcriptional levels of <i>CaWRKY6</i>, <i>CaWRKY22</i>, <i>CaWRKY27</i>, and <i>CaWRKY40</i>. On the other hand, transient over-expression of <i>CaWRKY6</i>, <i>CaWRKY22</i>, <i>CaWRKY27</i>, and <i>CaWRKY40</i> upregulated transcriptional expression levels of <i>CaWRKY30</i>. The results recommend that newly characterized <i>CaWRKY30</i> positively regulates pepper’s immunity against <i>Ralstonia</i> attack, which is governed by synergistically mediated signaling by phytohormones like ET, ABA, and SA, and transcriptionally assimilating into WRKY TFs networks, consisting of <i>CaWRKY6</i>, <i>CaWRKY22</i>, <i>CaWRKY27</i>, and <i>CaWRKY40</i>. Collectively, our data will facilitate to explicate the underlying mechanism of crosstalk between pepper’s immunity and response to RSI. |
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