Use of DNA barcode in the identification of catfishes (Siluriformes: Ariidae) from Malaysia

Abdullah ML, Nor SAM, Naim DMd. 2017. Use of DNA barcode in the identification of catfishes (Siluriformes: Ariidae) from Malaysia. Biodiversitas 18: 1358-1366. The genus Ariidae contains many valuable fish species threatened by overfishing, but knowledge on distribution and threats is still limited...

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Autores principales:	MOHD LUTFI ABDULLAH, SITI AZIZAH MOHD NOR, DARLINA MD. NAIM
Formato:	article
Lenguaje:	EN
Publicado:	MBI & UNS Solo 2017
Materias:	ariidae coi dna barcoding phylogenetic conservation Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/0d903435ecdf49e598538132d90b491b
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spelling	oai:doaj.org-article:0d903435ecdf49e598538132d90b491b2021-11-16T14:00:13ZUse of DNA barcode in the identification of catfishes (Siluriformes: Ariidae) from Malaysia1412-033X2085-472210.13057/biodiv/d180411https://doaj.org/article/0d903435ecdf49e598538132d90b491b2017-10-01T00:00:00Zhttps://smujo.id/biodiv/article/view/2066https://doaj.org/toc/1412-033Xhttps://doaj.org/toc/2085-4722Abdullah ML, Nor SAM, Naim DMd. 2017. Use of DNA barcode in the identification of catfishes (Siluriformes: Ariidae) from Malaysia. Biodiversitas 18: 1358-1366. The genus Ariidae contains many valuable fish species threatened by overfishing, but knowledge on distribution and threats is still limited due to taxonomic ambiguities. The aim of this study was to apply DNA barcoding techniques to establish a resource of DNA for identification of Ariidae species in Malaysia. A 621 bp of mitochondrial cytochrome oxidase subunit I (COI) gene was utilized to resolve phylogenetic relationships and molecular taxonomy of eight presumed Malaysian Ariid species. We found the monophyly of most species was well established with a mean Kimura-2 parameter (K2P) interspecies distance of 9.6% except for two species, Arius venosus, and Nemapteryx caelata that have very low interspecies genetic distance. The BLAST result shows only two species matched the presumably eight identified fish species. Such discrepancies could arise as a result of misidentifications or errors in GenBank database input, hybridization or incomplete lineage sorting. We suggest the use of DNA barcoding is integrated into the workflow during taxonomic studies as it could significantly increase knowledge about species distributions.MOHD LUTFI ABDULLAHSITI AZIZAH MOHD NORDARLINA MD. NAIMMBI & UNS Soloarticleariidaecoidna barcodingphylogeneticconservationBiology (General)QH301-705.5ENBiodiversitas, Vol 18, Iss 4, Pp 1358-1366 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	ariidae coi dna barcoding phylogenetic conservation Biology (General) QH301-705.5
spellingShingle	ariidae coi dna barcoding phylogenetic conservation Biology (General) QH301-705.5 MOHD LUTFI ABDULLAH SITI AZIZAH MOHD NOR DARLINA MD. NAIM Use of DNA barcode in the identification of catfishes (Siluriformes: Ariidae) from Malaysia
description	Abdullah ML, Nor SAM, Naim DMd. 2017. Use of DNA barcode in the identification of catfishes (Siluriformes: Ariidae) from Malaysia. Biodiversitas 18: 1358-1366. The genus Ariidae contains many valuable fish species threatened by overfishing, but knowledge on distribution and threats is still limited due to taxonomic ambiguities. The aim of this study was to apply DNA barcoding techniques to establish a resource of DNA for identification of Ariidae species in Malaysia. A 621 bp of mitochondrial cytochrome oxidase subunit I (COI) gene was utilized to resolve phylogenetic relationships and molecular taxonomy of eight presumed Malaysian Ariid species. We found the monophyly of most species was well established with a mean Kimura-2 parameter (K2P) interspecies distance of 9.6% except for two species, Arius venosus, and Nemapteryx caelata that have very low interspecies genetic distance. The BLAST result shows only two species matched the presumably eight identified fish species. Such discrepancies could arise as a result of misidentifications or errors in GenBank database input, hybridization or incomplete lineage sorting. We suggest the use of DNA barcoding is integrated into the workflow during taxonomic studies as it could significantly increase knowledge about species distributions.
format	article
author	MOHD LUTFI ABDULLAH SITI AZIZAH MOHD NOR DARLINA MD. NAIM
author_facet	MOHD LUTFI ABDULLAH SITI AZIZAH MOHD NOR DARLINA MD. NAIM
author_sort	MOHD LUTFI ABDULLAH
title	Use of DNA barcode in the identification of catfishes (Siluriformes: Ariidae) from Malaysia
title_short	Use of DNA barcode in the identification of catfishes (Siluriformes: Ariidae) from Malaysia
title_full	Use of DNA barcode in the identification of catfishes (Siluriformes: Ariidae) from Malaysia
title_fullStr	Use of DNA barcode in the identification of catfishes (Siluriformes: Ariidae) from Malaysia
title_full_unstemmed	Use of DNA barcode in the identification of catfishes (Siluriformes: Ariidae) from Malaysia
title_sort	use of dna barcode in the identification of catfishes (siluriformes: ariidae) from malaysia
publisher	MBI & UNS Solo
publishDate	2017
url	https://doaj.org/article/0d903435ecdf49e598538132d90b491b
work_keys_str_mv	AT mohdlutfiabdullah useofdnabarcodeintheidentificationofcatfishessiluriformesariidaefrommalaysia AT sitiazizahmohdnor useofdnabarcodeintheidentificationofcatfishessiluriformesariidaefrommalaysia AT darlinamdnaim useofdnabarcodeintheidentificationofcatfishessiluriformesariidaefrommalaysia
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Use of DNA barcode in the identification of catfishes (Siluriformes: Ariidae) from Malaysia

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