Characterising side chains in large proteins by protonless 13C-detected NMR spectroscopy
Analysis of side-chain motions by NMR has so far been restricted to small proteins and methyl-bearing side chains. Here, the authors present NMR methods based on 13C direct detection of highly deuterated protein samples that yield sharp and well-resolved signals and allow the characterisation of sid...
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Formato: | article |
Lenguaje: | EN |
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Nature Portfolio
2019
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Acceso en línea: | https://doaj.org/article/0da10a54a94e459fb2afae5adb60909a |
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