Characterising side chains in large proteins by protonless 13C-detected NMR spectroscopy

Analysis of side-chain motions by NMR has so far been restricted to small proteins and methyl-bearing side chains. Here, the authors present NMR methods based on 13C direct detection of highly deuterated protein samples that yield sharp and well-resolved signals and allow the characterisation of sid...

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Auteurs principaux: Ruth B. Pritchard, D. Flemming Hansen
Format: article
Langue:EN
Publié: Nature Portfolio 2019
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Q
Accès en ligne:https://doaj.org/article/0da10a54a94e459fb2afae5adb60909a
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