CircMMP11 overexpression predicts the poor survival of non-small cell lung cancer and downregulates miR-143 through methylation to suppress cell proliferation

Abstract Background CircMMP11 is a characterized circRNA with oncogenic function in breast cancer. In this study, we explored the involvement of circMMP11 in non-small cell lung cancer (NSCLC). Methods Paired cancer and non-cancer tissues were collected from 66 NSCLC patients, and the expression of...

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Autores principales:	Juan Chen, Jiang Gong
Formato:	article
Lenguaje:	EN
Publicado:	BMC 2021
Materias:	Non-small cell lung cancer circMMP11 miR-143 Methylation Surgery RD1-811 Anesthesiology RD78.3-87.3
Acceso en línea:	https://doaj.org/article/0da296606d01413da669a5f83b2b6606
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spelling	oai:doaj.org-article:0da296606d01413da669a5f83b2b66062021-11-14T12:33:51ZCircMMP11 overexpression predicts the poor survival of non-small cell lung cancer and downregulates miR-143 through methylation to suppress cell proliferation10.1186/s13019-021-01701-w1749-8090https://doaj.org/article/0da296606d01413da669a5f83b2b66062021-11-01T00:00:00Zhttps://doi.org/10.1186/s13019-021-01701-whttps://doaj.org/toc/1749-8090Abstract Background CircMMP11 is a characterized circRNA with oncogenic function in breast cancer. In this study, we explored the involvement of circMMP11 in non-small cell lung cancer (NSCLC). Methods Paired cancer and non-cancer tissues were collected from 66 NSCLC patients, and the expression of circMMP11 and miR-143 in these tissues were detected using RT-qPCRs. Overexpression levels of circMMP11 and miR-143 were performed by transfection, and their crosstalk was analyzed by RT-qPCRs. The effect of circMMP11 overexpression on miR-143 methylation was analyzed by methylation-specific PCR. CCK-8 assay was performed to analyze the roles of miR-143 and circMMP11 in regulating NSCLC cell proliferation. Results We found that circMMP11 was overexpressed in NSCLC and predicted patients’ poor survival. Moreover, a close correlation between circMMP11 and miR143 was observed. In NSCLC cells, circMMP11 overexpression reduced miR-143 expression and increased miR-143 methylation. CCK-8 assay analysis showed that miR-143 reversed the enhancing effects of circMMP11 overexpression on cell proliferation. Conclusions CircMMP11 is overexpressed in NSCLC and predicts poor survival. In addition, circMMP11 may downregulate miR-143 through methylation to suppress cell proliferation.Juan ChenJiang GongBMCarticleNon-small cell lung cancercircMMP11miR-143MethylationSurgeryRD1-811AnesthesiologyRD78.3-87.3ENJournal of Cardiothoracic Surgery, Vol 16, Iss 1, Pp 1-6 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Non-small cell lung cancer circMMP11 miR-143 Methylation Surgery RD1-811 Anesthesiology RD78.3-87.3
spellingShingle	Non-small cell lung cancer circMMP11 miR-143 Methylation Surgery RD1-811 Anesthesiology RD78.3-87.3 Juan Chen Jiang Gong CircMMP11 overexpression predicts the poor survival of non-small cell lung cancer and downregulates miR-143 through methylation to suppress cell proliferation
description	Abstract Background CircMMP11 is a characterized circRNA with oncogenic function in breast cancer. In this study, we explored the involvement of circMMP11 in non-small cell lung cancer (NSCLC). Methods Paired cancer and non-cancer tissues were collected from 66 NSCLC patients, and the expression of circMMP11 and miR-143 in these tissues were detected using RT-qPCRs. Overexpression levels of circMMP11 and miR-143 were performed by transfection, and their crosstalk was analyzed by RT-qPCRs. The effect of circMMP11 overexpression on miR-143 methylation was analyzed by methylation-specific PCR. CCK-8 assay was performed to analyze the roles of miR-143 and circMMP11 in regulating NSCLC cell proliferation. Results We found that circMMP11 was overexpressed in NSCLC and predicted patients’ poor survival. Moreover, a close correlation between circMMP11 and miR143 was observed. In NSCLC cells, circMMP11 overexpression reduced miR-143 expression and increased miR-143 methylation. CCK-8 assay analysis showed that miR-143 reversed the enhancing effects of circMMP11 overexpression on cell proliferation. Conclusions CircMMP11 is overexpressed in NSCLC and predicts poor survival. In addition, circMMP11 may downregulate miR-143 through methylation to suppress cell proliferation.
format	article
author	Juan Chen Jiang Gong
author_facet	Juan Chen Jiang Gong
author_sort	Juan Chen
title	CircMMP11 overexpression predicts the poor survival of non-small cell lung cancer and downregulates miR-143 through methylation to suppress cell proliferation
title_short	CircMMP11 overexpression predicts the poor survival of non-small cell lung cancer and downregulates miR-143 through methylation to suppress cell proliferation
title_full	CircMMP11 overexpression predicts the poor survival of non-small cell lung cancer and downregulates miR-143 through methylation to suppress cell proliferation
title_fullStr	CircMMP11 overexpression predicts the poor survival of non-small cell lung cancer and downregulates miR-143 through methylation to suppress cell proliferation
title_full_unstemmed	CircMMP11 overexpression predicts the poor survival of non-small cell lung cancer and downregulates miR-143 through methylation to suppress cell proliferation
title_sort	circmmp11 overexpression predicts the poor survival of non-small cell lung cancer and downregulates mir-143 through methylation to suppress cell proliferation
publisher	BMC
publishDate	2021
url	https://doaj.org/article/0da296606d01413da669a5f83b2b6606
work_keys_str_mv	AT juanchen circmmp11overexpressionpredictsthepoorsurvivalofnonsmallcelllungcanceranddownregulatesmir143throughmethylationtosuppresscellproliferation AT jianggong circmmp11overexpressionpredictsthepoorsurvivalofnonsmallcelllungcanceranddownregulatesmir143throughmethylationtosuppresscellproliferation
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CircMMP11 overexpression predicts the poor survival of non-small cell lung cancer and downregulates miR-143 through methylation to suppress cell proliferation

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