Targeted Manipulation of Abundant and Rare Taxa in the <italic toggle="yes">Daphnia magna</italic> Microbiota with Antibiotics Impacts Host Fitness Differentially

ABSTRACT Host-associated microbes contribute to host fitness, but it is unclear whether these contributions are from rare keystone taxa, numerically abundant taxa, or interactions among community members. Experimental perturbation of the microbiota can highlight functionally important taxa; however,...

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Autores principales:	Reilly O. Cooper, Janna M. Vavra, Clayton E. Cressler
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2021
Materias:	microbiome invertebrate-microbe interactions Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/0da46524294f492db6d1be5716d1d805
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spelling	oai:doaj.org-article:0da46524294f492db6d1be5716d1d8052021-12-02T19:36:39ZTargeted Manipulation of Abundant and Rare Taxa in the <italic toggle="yes">Daphnia magna</italic> Microbiota with Antibiotics Impacts Host Fitness Differentially10.1128/mSystems.00916-202379-5077https://doaj.org/article/0da46524294f492db6d1be5716d1d8052021-04-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mSystems.00916-20https://doaj.org/toc/2379-5077ABSTRACT Host-associated microbes contribute to host fitness, but it is unclear whether these contributions are from rare keystone taxa, numerically abundant taxa, or interactions among community members. Experimental perturbation of the microbiota can highlight functionally important taxa; however, this approach is primarily applied in systems with complex communities where the perturbation affects hundreds of taxa, making it difficult to pinpoint contributions of key community members. Here, we use the ecological model organism Daphnia magna to examine the importance of rare and abundant taxa by perturbing its relatively simple microbiota with targeted antibiotics. We used sublethal antibiotic doses to target either rare or abundant members across two temperatures and then measured key host life history metrics and shifts in microbial community composition. We find that removal of abundant taxa had greater impacts on host fitness than did removal of rare taxa and that the abundances of nontarget taxa were impacted by antibiotic treatment, suggesting that no rare keystone taxa exist in the Daphnia magna microbiota but that microbe-microbe interactions may play a role in host fitness. We also find that microbial community composition was impacted by antibiotics differently across temperatures, indicating that ecological context shapes within-host microbial responses and effects on host fitness. IMPORTANCE Understanding the contributions of rare and abundant taxa to host fitness is an outstanding question in host microbial ecology. In this study, we use the model zooplankton Daphnia magna and its relatively simple cohort of bacterial taxa to disentangle the roles of distinct taxa in host life history metrics, using a suite of antibiotics to selectively reduce the abundance of functionally important taxa. We also examine how environmental context shapes the importance of these bacterial taxa in host fitness.Reilly O. CooperJanna M. VavraClayton E. CresslerAmerican Society for Microbiologyarticlemicrobiomeinvertebrate-microbe interactionsMicrobiologyQR1-502ENmSystems, Vol 6, Iss 2 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	microbiome invertebrate-microbe interactions Microbiology QR1-502
spellingShingle	microbiome invertebrate-microbe interactions Microbiology QR1-502 Reilly O. Cooper Janna M. Vavra Clayton E. Cressler Targeted Manipulation of Abundant and Rare Taxa in the <italic toggle="yes">Daphnia magna</italic> Microbiota with Antibiotics Impacts Host Fitness Differentially
description	ABSTRACT Host-associated microbes contribute to host fitness, but it is unclear whether these contributions are from rare keystone taxa, numerically abundant taxa, or interactions among community members. Experimental perturbation of the microbiota can highlight functionally important taxa; however, this approach is primarily applied in systems with complex communities where the perturbation affects hundreds of taxa, making it difficult to pinpoint contributions of key community members. Here, we use the ecological model organism Daphnia magna to examine the importance of rare and abundant taxa by perturbing its relatively simple microbiota with targeted antibiotics. We used sublethal antibiotic doses to target either rare or abundant members across two temperatures and then measured key host life history metrics and shifts in microbial community composition. We find that removal of abundant taxa had greater impacts on host fitness than did removal of rare taxa and that the abundances of nontarget taxa were impacted by antibiotic treatment, suggesting that no rare keystone taxa exist in the Daphnia magna microbiota but that microbe-microbe interactions may play a role in host fitness. We also find that microbial community composition was impacted by antibiotics differently across temperatures, indicating that ecological context shapes within-host microbial responses and effects on host fitness. IMPORTANCE Understanding the contributions of rare and abundant taxa to host fitness is an outstanding question in host microbial ecology. In this study, we use the model zooplankton Daphnia magna and its relatively simple cohort of bacterial taxa to disentangle the roles of distinct taxa in host life history metrics, using a suite of antibiotics to selectively reduce the abundance of functionally important taxa. We also examine how environmental context shapes the importance of these bacterial taxa in host fitness.
format	article
author	Reilly O. Cooper Janna M. Vavra Clayton E. Cressler
author_facet	Reilly O. Cooper Janna M. Vavra Clayton E. Cressler
author_sort	Reilly O. Cooper
title	Targeted Manipulation of Abundant and Rare Taxa in the <italic toggle="yes">Daphnia magna</italic> Microbiota with Antibiotics Impacts Host Fitness Differentially
title_short	Targeted Manipulation of Abundant and Rare Taxa in the <italic toggle="yes">Daphnia magna</italic> Microbiota with Antibiotics Impacts Host Fitness Differentially
title_full	Targeted Manipulation of Abundant and Rare Taxa in the <italic toggle="yes">Daphnia magna</italic> Microbiota with Antibiotics Impacts Host Fitness Differentially
title_fullStr	Targeted Manipulation of Abundant and Rare Taxa in the <italic toggle="yes">Daphnia magna</italic> Microbiota with Antibiotics Impacts Host Fitness Differentially
title_full_unstemmed	Targeted Manipulation of Abundant and Rare Taxa in the <italic toggle="yes">Daphnia magna</italic> Microbiota with Antibiotics Impacts Host Fitness Differentially
title_sort	targeted manipulation of abundant and rare taxa in the <italic toggle="yes">daphnia magna</italic> microbiota with antibiotics impacts host fitness differentially
publisher	American Society for Microbiology
publishDate	2021
url	https://doaj.org/article/0da46524294f492db6d1be5716d1d805
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Targeted Manipulation of Abundant and Rare Taxa in the <italic toggle="yes">Daphnia magna</italic> Microbiota with Antibiotics Impacts Host Fitness Differentially

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