Efficient production of vindoline from tabersonine by metabolically engineered Saccharomyces cerevisiae

Liu et al. optimize vindoline biosynthesis in yeast from tabersonine by altering the copy numbers of pathway genes, pairing CYPs with CPRs, engineering the microenvironment for functional expression of CYPs, enhancing cofactor supplies, and optimizing fermentation conditions. By doing so, the author...

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Auteurs principaux:	Tengfei Liu, Ying Huang, Lihong Jiang, Chang Dong, Yuanwei Gou, Jiazhang Lian
Format:	article
Langue:	EN
Publié:	Nature Portfolio 2021
Sujets:	Biology (General) QH301-705.5
Accès en ligne:	https://doaj.org/article/0daa35ca313a41a7b7ca2d89a0328a01
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spelling	oai:doaj.org-article:0daa35ca313a41a7b7ca2d89a0328a012021-12-02T18:50:02ZEfficient production of vindoline from tabersonine by metabolically engineered Saccharomyces cerevisiae10.1038/s42003-021-02617-w2399-3642https://doaj.org/article/0daa35ca313a41a7b7ca2d89a0328a012021-09-01T00:00:00Zhttps://doi.org/10.1038/s42003-021-02617-whttps://doaj.org/toc/2399-3642Liu et al. optimize vindoline biosynthesis in yeast from tabersonine by altering the copy numbers of pathway genes, pairing CYPs with CPRs, engineering the microenvironment for functional expression of CYPs, enhancing cofactor supplies, and optimizing fermentation conditions. By doing so, the authors increased the production of vindoline to a final titer as high as ∼16.5 mg/L.Tengfei LiuYing HuangLihong JiangChang DongYuanwei GouJiazhang LianNature PortfolioarticleBiology (General)QH301-705.5ENCommunications Biology, Vol 4, Iss 1, Pp 1-9 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Biology (General) QH301-705.5
spellingShingle	Biology (General) QH301-705.5 Tengfei Liu Ying Huang Lihong Jiang Chang Dong Yuanwei Gou Jiazhang Lian Efficient production of vindoline from tabersonine by metabolically engineered Saccharomyces cerevisiae
description	Liu et al. optimize vindoline biosynthesis in yeast from tabersonine by altering the copy numbers of pathway genes, pairing CYPs with CPRs, engineering the microenvironment for functional expression of CYPs, enhancing cofactor supplies, and optimizing fermentation conditions. By doing so, the authors increased the production of vindoline to a final titer as high as ∼16.5 mg/L.
format	article
author	Tengfei Liu Ying Huang Lihong Jiang Chang Dong Yuanwei Gou Jiazhang Lian
author_facet	Tengfei Liu Ying Huang Lihong Jiang Chang Dong Yuanwei Gou Jiazhang Lian
author_sort	Tengfei Liu
title	Efficient production of vindoline from tabersonine by metabolically engineered Saccharomyces cerevisiae
title_short	Efficient production of vindoline from tabersonine by metabolically engineered Saccharomyces cerevisiae
title_full	Efficient production of vindoline from tabersonine by metabolically engineered Saccharomyces cerevisiae
title_fullStr	Efficient production of vindoline from tabersonine by metabolically engineered Saccharomyces cerevisiae
title_full_unstemmed	Efficient production of vindoline from tabersonine by metabolically engineered Saccharomyces cerevisiae
title_sort	efficient production of vindoline from tabersonine by metabolically engineered saccharomyces cerevisiae
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/0daa35ca313a41a7b7ca2d89a0328a01
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_version_	1718377485708558336

Efficient production of vindoline from tabersonine by metabolically engineered Saccharomyces cerevisiae

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