Effects of preanalytical frozen storage time and temperature on screening coagulation tests and factors VIII and IX activity

Abstract Preanalytical quality control of blood samples is critical for tests of coagulation function and coagulation factor activity. Preanalytical storage time and temperature are the main variables. We investigated the effects of preanalytical frozen storage time and temperature on activated part...

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Autores principales:	Ying Zhao, Guofang Feng, Jie Zhang, Renjie Gong, Changming Cai, Limin Feng
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/0dba97e8dae047ab9dcef84468a3a35c
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spelling	oai:doaj.org-article:0dba97e8dae047ab9dcef84468a3a35c2021-12-02T11:52:28ZEffects of preanalytical frozen storage time and temperature on screening coagulation tests and factors VIII and IX activity10.1038/s41598-017-11777-x2045-2322https://doaj.org/article/0dba97e8dae047ab9dcef84468a3a35c2017-09-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-11777-xhttps://doaj.org/toc/2045-2322Abstract Preanalytical quality control of blood samples is critical for tests of coagulation function and coagulation factor activity. Preanalytical storage time and temperature are the main variables. We investigated the effects of preanalytical frozen storage time and temperature on activated partial thromboplastin time (APTT), fibrinogen (Fbg), prothrombin time (PT)/international normalized ratio (INR), thrombin time (TT), factor VIII activity (FVIII:C), and factor IX activity (FIX:C) in frozen plasma. Samples (n = 144) were randomly and equally divided into four groups (storage at −80 °C or −20 °C) and analysed by CS5100 or CA7000 coagulation analysers. Baseline values and results after storage for 15 days, 1 month, 3 months, 6 months, and 1 year were measured after thawing. Mean percent changes and scatter plots were used to determine clinically relevant differences. The stabilities of coagulation tests and coagulation factor activities measured by the CS5100 system were consistent with those measured by the CA7000 system. At −80 °C, assessment samples of PT/INR, Fbg, and TT can be safely stored for 1 year, APTT for 6 months, and FVIII:C and FIX:C for 1 month. At −20 °C, samples of Fbg and TT can be stored for 1 year, PT/INR and FIX:C for 1 month, and APTT and FVIII:C for 15 days.Ying ZhaoGuofang FengJie ZhangRenjie GongChangming CaiLimin FengNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-9 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Ying Zhao Guofang Feng Jie Zhang Renjie Gong Changming Cai Limin Feng Effects of preanalytical frozen storage time and temperature on screening coagulation tests and factors VIII and IX activity
description	Abstract Preanalytical quality control of blood samples is critical for tests of coagulation function and coagulation factor activity. Preanalytical storage time and temperature are the main variables. We investigated the effects of preanalytical frozen storage time and temperature on activated partial thromboplastin time (APTT), fibrinogen (Fbg), prothrombin time (PT)/international normalized ratio (INR), thrombin time (TT), factor VIII activity (FVIII:C), and factor IX activity (FIX:C) in frozen plasma. Samples (n = 144) were randomly and equally divided into four groups (storage at −80 °C or −20 °C) and analysed by CS5100 or CA7000 coagulation analysers. Baseline values and results after storage for 15 days, 1 month, 3 months, 6 months, and 1 year were measured after thawing. Mean percent changes and scatter plots were used to determine clinically relevant differences. The stabilities of coagulation tests and coagulation factor activities measured by the CS5100 system were consistent with those measured by the CA7000 system. At −80 °C, assessment samples of PT/INR, Fbg, and TT can be safely stored for 1 year, APTT for 6 months, and FVIII:C and FIX:C for 1 month. At −20 °C, samples of Fbg and TT can be stored for 1 year, PT/INR and FIX:C for 1 month, and APTT and FVIII:C for 15 days.
format	article
author	Ying Zhao Guofang Feng Jie Zhang Renjie Gong Changming Cai Limin Feng
author_facet	Ying Zhao Guofang Feng Jie Zhang Renjie Gong Changming Cai Limin Feng
author_sort	Ying Zhao
title	Effects of preanalytical frozen storage time and temperature on screening coagulation tests and factors VIII and IX activity
title_short	Effects of preanalytical frozen storage time and temperature on screening coagulation tests and factors VIII and IX activity
title_full	Effects of preanalytical frozen storage time and temperature on screening coagulation tests and factors VIII and IX activity
title_fullStr	Effects of preanalytical frozen storage time and temperature on screening coagulation tests and factors VIII and IX activity
title_full_unstemmed	Effects of preanalytical frozen storage time and temperature on screening coagulation tests and factors VIII and IX activity
title_sort	effects of preanalytical frozen storage time and temperature on screening coagulation tests and factors viii and ix activity
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/0dba97e8dae047ab9dcef84468a3a35c
work_keys_str_mv	AT yingzhao effectsofpreanalyticalfrozenstoragetimeandtemperatureonscreeningcoagulationtestsandfactorsviiiandixactivity AT guofangfeng effectsofpreanalyticalfrozenstoragetimeandtemperatureonscreeningcoagulationtestsandfactorsviiiandixactivity AT jiezhang effectsofpreanalyticalfrozenstoragetimeandtemperatureonscreeningcoagulationtestsandfactorsviiiandixactivity AT renjiegong effectsofpreanalyticalfrozenstoragetimeandtemperatureonscreeningcoagulationtestsandfactorsviiiandixactivity AT changmingcai effectsofpreanalyticalfrozenstoragetimeandtemperatureonscreeningcoagulationtestsandfactorsviiiandixactivity AT liminfeng effectsofpreanalyticalfrozenstoragetimeandtemperatureonscreeningcoagulationtestsandfactorsviiiandixactivity
_version_	1718395029696806912

Effects of preanalytical frozen storage time and temperature on screening coagulation tests and factors VIII and IX activity

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