Robust behavioural effects in response to acute, but not repeated, terpene administration in Zebrafish (Danio rerio)

Abstract Terpenes are fragrant aromatic compounds produced by a variety of plants, most notably cannabis and hops. With increasing legalization of cannabis there is a need to better understand the behavioural effects of terpenes and ultimately their therapeutic value. Our study investigated the dose...

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Autores principales: Joshua Szaszkiewicz, Shannon Leigh, Trevor J. Hamilton
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Publicado: Nature Portfolio 2021
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spelling oai:doaj.org-article:0dbabc95b9c046f7a73bfa6fa40ab7e02021-12-02T18:51:36ZRobust behavioural effects in response to acute, but not repeated, terpene administration in Zebrafish (Danio rerio)10.1038/s41598-021-98768-12045-2322https://doaj.org/article/0dbabc95b9c046f7a73bfa6fa40ab7e02021-09-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-98768-1https://doaj.org/toc/2045-2322Abstract Terpenes are fragrant aromatic compounds produced by a variety of plants, most notably cannabis and hops. With increasing legalization of cannabis there is a need to better understand the behavioural effects of terpenes and ultimately their therapeutic value. Our study investigated the dose-dependent impact of three terpenes (limonene 0.25, 0.5, 0.75%; β-myrcene 0.001, 0.01, 0.1%; and 0.0001, 0.001, 0.00125% linalool) on zebrafish (Danio rerio) behaviour when exposed both acutely and repeatedly over a 7-day period. Anxiety-like behaviour, boldness, and locomotion were assessed using the open field test and the novel object approach test. In the acute dosing experiment, limonene and β-myrcene exposed groups demonstrated a significant decrease in locomotion, a decrease in anxiety-like behaviour, and an increase in boldness, while linalool treatment groups demonstrated only minor alterations in locomotion. Moreover, repeated exposure to limonene (0.39%) or β-myrcene (0.0083%) for a seven day period did not result in any significant behavioural effects. In conclusion, our study provides support for an anxiolytic and sedative effect in zebrafish in response to acute limonene and β-myrcene exposure that is no longer present after one week of repeated exposure.Joshua SzaszkiewiczShannon LeighTrevor J. HamiltonNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-17 (2021)
institution DOAJ
collection DOAJ
language EN
topic Medicine
R
Science
Q
spellingShingle Medicine
R
Science
Q
Joshua Szaszkiewicz
Shannon Leigh
Trevor J. Hamilton
Robust behavioural effects in response to acute, but not repeated, terpene administration in Zebrafish (Danio rerio)
description Abstract Terpenes are fragrant aromatic compounds produced by a variety of plants, most notably cannabis and hops. With increasing legalization of cannabis there is a need to better understand the behavioural effects of terpenes and ultimately their therapeutic value. Our study investigated the dose-dependent impact of three terpenes (limonene 0.25, 0.5, 0.75%; β-myrcene 0.001, 0.01, 0.1%; and 0.0001, 0.001, 0.00125% linalool) on zebrafish (Danio rerio) behaviour when exposed both acutely and repeatedly over a 7-day period. Anxiety-like behaviour, boldness, and locomotion were assessed using the open field test and the novel object approach test. In the acute dosing experiment, limonene and β-myrcene exposed groups demonstrated a significant decrease in locomotion, a decrease in anxiety-like behaviour, and an increase in boldness, while linalool treatment groups demonstrated only minor alterations in locomotion. Moreover, repeated exposure to limonene (0.39%) or β-myrcene (0.0083%) for a seven day period did not result in any significant behavioural effects. In conclusion, our study provides support for an anxiolytic and sedative effect in zebrafish in response to acute limonene and β-myrcene exposure that is no longer present after one week of repeated exposure.
format article
author Joshua Szaszkiewicz
Shannon Leigh
Trevor J. Hamilton
author_facet Joshua Szaszkiewicz
Shannon Leigh
Trevor J. Hamilton
author_sort Joshua Szaszkiewicz
title Robust behavioural effects in response to acute, but not repeated, terpene administration in Zebrafish (Danio rerio)
title_short Robust behavioural effects in response to acute, but not repeated, terpene administration in Zebrafish (Danio rerio)
title_full Robust behavioural effects in response to acute, but not repeated, terpene administration in Zebrafish (Danio rerio)
title_fullStr Robust behavioural effects in response to acute, but not repeated, terpene administration in Zebrafish (Danio rerio)
title_full_unstemmed Robust behavioural effects in response to acute, but not repeated, terpene administration in Zebrafish (Danio rerio)
title_sort robust behavioural effects in response to acute, but not repeated, terpene administration in zebrafish (danio rerio)
publisher Nature Portfolio
publishDate 2021
url https://doaj.org/article/0dbabc95b9c046f7a73bfa6fa40ab7e0
work_keys_str_mv AT joshuaszaszkiewicz robustbehaviouraleffectsinresponsetoacutebutnotrepeatedterpeneadministrationinzebrafishdaniorerio
AT shannonleigh robustbehaviouraleffectsinresponsetoacutebutnotrepeatedterpeneadministrationinzebrafishdaniorerio
AT trevorjhamilton robustbehaviouraleffectsinresponsetoacutebutnotrepeatedterpeneadministrationinzebrafishdaniorerio
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