Quantitative assay of Indole Acetic Acid-producing bacteria isolated from several lakes in East Java, Indonesia

Abstract. Ramadhani SI, Prabaningtyas S, Witjoro A, Saptawati TR, Rodiansyah A. 2020. Quantitative assay of Indole Acetic Acid-producing bacteria isolated from several lakes in East Java, Indonesia. Biodiversitas 21: 5448-5454. Biofuel is an alternative to fossil fuels that are environmentally frien...

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Autores principales:	Sulistya Ika Ramadhani Sulistya, Sitoresmi Prabaningtyas Sitoresmi, Agung Witjoro Agung, Rina Triturani Saptawati Rina, Achmad Rodiansyah Achmad
Formato:	article
Lenguaje:	EN
Publicado:	MBI & UNS Solo 2020
Materias:	biofuels, east java, iaa-producing bacteria, lakes Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/0dbf142b7eea463da21bb5327afbc10f
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spelling	oai:doaj.org-article:0dbf142b7eea463da21bb5327afbc10f2021-11-22T00:44:10ZQuantitative assay of Indole Acetic Acid-producing bacteria isolated from several lakes in East Java, Indonesia1412-033X2085-472210.13057/biodiv/d211153https://doaj.org/article/0dbf142b7eea463da21bb5327afbc10f2020-10-01T00:00:00Zhttps://smujo.id/biodiv/article/view/6746https://doaj.org/toc/1412-033Xhttps://doaj.org/toc/2085-4722Abstract. Ramadhani SI, Prabaningtyas S, Witjoro A, Saptawati TR, Rodiansyah A. 2020. Quantitative assay of Indole Acetic Acid-producing bacteria isolated from several lakes in East Java, Indonesia. Biodiversitas 21: 5448-5454. Biofuel is an alternative to fossil fuels that are environmentally friendly with low emissions. Biofuel from biomass microalgae, especially Chlorella vulgaris, has an essential role in biofuel production. Increasing biomass microalgae was done by co-culture between microalgae and bacteria. This research aims to determine the potential of bacterial isolates to produce the IAA hormone and identify the highest isolate with the ability to synthesis IAA from four lakes in East Java. This research was conducted by culturing bacterial isolates in the Tryptic Soy Broth (TSB) to add tryptophan media in various periods of incubation. The absorbance was measured with UV-Vis spectrophotometry at a wavelength of 530 nm for determining IAA-production from bacterial isolates. The results showed that the "12" code bacterial isolate from Ranu Grati produced the highest IAA hormone concentration, with an average of 30.23 ppm. The morphological characterization of the highest IAA-producing bacteria showed that isolate included the Enterobacteriaceae group and phenotypic characterization include Enterobacter cloacae complex (ECC).Sulistya Ika Ramadhani SulistyaSitoresmi Prabaningtyas SitoresmiAgung Witjoro AgungRina Triturani Saptawati RinaAchmad Rodiansyah AchmadMBI & UNS Soloarticlebiofuels, east java, iaa-producing bacteria, lakesBiology (General)QH301-705.5ENBiodiversitas, Vol 21, Iss 11 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	biofuels, east java, iaa-producing bacteria, lakes Biology (General) QH301-705.5
spellingShingle	biofuels, east java, iaa-producing bacteria, lakes Biology (General) QH301-705.5 Sulistya Ika Ramadhani Sulistya Sitoresmi Prabaningtyas Sitoresmi Agung Witjoro Agung Rina Triturani Saptawati Rina Achmad Rodiansyah Achmad Quantitative assay of Indole Acetic Acid-producing bacteria isolated from several lakes in East Java, Indonesia
description	Abstract. Ramadhani SI, Prabaningtyas S, Witjoro A, Saptawati TR, Rodiansyah A. 2020. Quantitative assay of Indole Acetic Acid-producing bacteria isolated from several lakes in East Java, Indonesia. Biodiversitas 21: 5448-5454. Biofuel is an alternative to fossil fuels that are environmentally friendly with low emissions. Biofuel from biomass microalgae, especially Chlorella vulgaris, has an essential role in biofuel production. Increasing biomass microalgae was done by co-culture between microalgae and bacteria. This research aims to determine the potential of bacterial isolates to produce the IAA hormone and identify the highest isolate with the ability to synthesis IAA from four lakes in East Java. This research was conducted by culturing bacterial isolates in the Tryptic Soy Broth (TSB) to add tryptophan media in various periods of incubation. The absorbance was measured with UV-Vis spectrophotometry at a wavelength of 530 nm for determining IAA-production from bacterial isolates. The results showed that the "12" code bacterial isolate from Ranu Grati produced the highest IAA hormone concentration, with an average of 30.23 ppm. The morphological characterization of the highest IAA-producing bacteria showed that isolate included the Enterobacteriaceae group and phenotypic characterization include Enterobacter cloacae complex (ECC).
format	article
author	Sulistya Ika Ramadhani Sulistya Sitoresmi Prabaningtyas Sitoresmi Agung Witjoro Agung Rina Triturani Saptawati Rina Achmad Rodiansyah Achmad
author_facet	Sulistya Ika Ramadhani Sulistya Sitoresmi Prabaningtyas Sitoresmi Agung Witjoro Agung Rina Triturani Saptawati Rina Achmad Rodiansyah Achmad
author_sort	Sulistya Ika Ramadhani Sulistya
title	Quantitative assay of Indole Acetic Acid-producing bacteria isolated from several lakes in East Java, Indonesia
title_short	Quantitative assay of Indole Acetic Acid-producing bacteria isolated from several lakes in East Java, Indonesia
title_full	Quantitative assay of Indole Acetic Acid-producing bacteria isolated from several lakes in East Java, Indonesia
title_fullStr	Quantitative assay of Indole Acetic Acid-producing bacteria isolated from several lakes in East Java, Indonesia
title_full_unstemmed	Quantitative assay of Indole Acetic Acid-producing bacteria isolated from several lakes in East Java, Indonesia
title_sort	quantitative assay of indole acetic acid-producing bacteria isolated from several lakes in east java, indonesia
publisher	MBI & UNS Solo
publishDate	2020
url	https://doaj.org/article/0dbf142b7eea463da21bb5327afbc10f
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Quantitative assay of Indole Acetic Acid-producing bacteria isolated from several lakes in East Java, Indonesia

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