Processing, Export, and Identification of Novel Linear Peptides from <named-content content-type="genus-species">Staphylococcus aureus</named-content>

ABSTRACT Staphylococcus aureus can colonize the human host and cause a variety of superficial and invasive infections. The success of S. aureus as a pathogen derives from its ability to modulate its virulence through the release, sensing of and response to cyclic signaling peptides. Here we provide,...

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Autores principales:	Katrin Schilcher, Lindsay K. Caesar, Nadja B. Cech, Alexander R. Horswill
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2020
Materias:	S. aureus linear peptides lipoproteins Eep EcsAB Staphylococcus aureus Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/0dc85f56e924413f98c6184685e5d0ec
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spelling	oai:doaj.org-article:0dc85f56e924413f98c6184685e5d0ec2021-11-15T15:57:03ZProcessing, Export, and Identification of Novel Linear Peptides from <named-content content-type="genus-species">Staphylococcus aureus</named-content>10.1128/mBio.00112-202150-7511https://doaj.org/article/0dc85f56e924413f98c6184685e5d0ec2020-04-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mBio.00112-20https://doaj.org/toc/2150-7511ABSTRACT Staphylococcus aureus can colonize the human host and cause a variety of superficial and invasive infections. The success of S. aureus as a pathogen derives from its ability to modulate its virulence through the release, sensing of and response to cyclic signaling peptides. Here we provide, for the first time, evidence that S. aureus processes and secretes small linear peptides through a specialized pathway that converts a lipoprotein leader into an extracellular peptide signal. We have identified and confirmed the machinery for each step and demonstrate that the putative membrane metalloprotease Eep and the EcsAB transporter are required to complete the processing and secretion of the peptides. In addition, we have identified several linear peptides, including the interspecies signaling molecule staph-cAM373, that are dependent on this processing and secretion pathway. These findings are particularly important because multiple Gram-positive bacteria rely on small linear peptides to control bacterial gene expression and virulence. IMPORTANCE Here, we provide evidence indicating that S. aureus secretes small linear peptides into the environment via a novel processing and secretion pathway. The discovery of a specialized pathway for the production of small linear peptides and the identification of these peptides leads to several important questions regarding their role in S. aureus biology, most interestingly, their potential to act as signaling molecules. The observations in this study provide a foundation for further in-depth studies into the biological activity of small linear peptides in S. aureus.Katrin SchilcherLindsay K. CaesarNadja B. CechAlexander R. HorswillAmerican Society for MicrobiologyarticleS. aureuslinear peptideslipoproteinsEepEcsABStaphylococcus aureusMicrobiologyQR1-502ENmBio, Vol 11, Iss 2 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	S. aureus linear peptides lipoproteins Eep EcsAB Staphylococcus aureus Microbiology QR1-502
spellingShingle	S. aureus linear peptides lipoproteins Eep EcsAB Staphylococcus aureus Microbiology QR1-502 Katrin Schilcher Lindsay K. Caesar Nadja B. Cech Alexander R. Horswill Processing, Export, and Identification of Novel Linear Peptides from <named-content content-type="genus-species">Staphylococcus aureus</named-content>
description	ABSTRACT Staphylococcus aureus can colonize the human host and cause a variety of superficial and invasive infections. The success of S. aureus as a pathogen derives from its ability to modulate its virulence through the release, sensing of and response to cyclic signaling peptides. Here we provide, for the first time, evidence that S. aureus processes and secretes small linear peptides through a specialized pathway that converts a lipoprotein leader into an extracellular peptide signal. We have identified and confirmed the machinery for each step and demonstrate that the putative membrane metalloprotease Eep and the EcsAB transporter are required to complete the processing and secretion of the peptides. In addition, we have identified several linear peptides, including the interspecies signaling molecule staph-cAM373, that are dependent on this processing and secretion pathway. These findings are particularly important because multiple Gram-positive bacteria rely on small linear peptides to control bacterial gene expression and virulence. IMPORTANCE Here, we provide evidence indicating that S. aureus secretes small linear peptides into the environment via a novel processing and secretion pathway. The discovery of a specialized pathway for the production of small linear peptides and the identification of these peptides leads to several important questions regarding their role in S. aureus biology, most interestingly, their potential to act as signaling molecules. The observations in this study provide a foundation for further in-depth studies into the biological activity of small linear peptides in S. aureus.
format	article
author	Katrin Schilcher Lindsay K. Caesar Nadja B. Cech Alexander R. Horswill
author_facet	Katrin Schilcher Lindsay K. Caesar Nadja B. Cech Alexander R. Horswill
author_sort	Katrin Schilcher
title	Processing, Export, and Identification of Novel Linear Peptides from <named-content content-type="genus-species">Staphylococcus aureus</named-content>
title_short	Processing, Export, and Identification of Novel Linear Peptides from <named-content content-type="genus-species">Staphylococcus aureus</named-content>
title_full	Processing, Export, and Identification of Novel Linear Peptides from <named-content content-type="genus-species">Staphylococcus aureus</named-content>
title_fullStr	Processing, Export, and Identification of Novel Linear Peptides from <named-content content-type="genus-species">Staphylococcus aureus</named-content>
title_full_unstemmed	Processing, Export, and Identification of Novel Linear Peptides from <named-content content-type="genus-species">Staphylococcus aureus</named-content>
title_sort	processing, export, and identification of novel linear peptides from <named-content content-type="genus-species">staphylococcus aureus</named-content>
publisher	American Society for Microbiology
publishDate	2020
url	https://doaj.org/article/0dc85f56e924413f98c6184685e5d0ec
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Processing, Export, and Identification of Novel Linear Peptides from <named-content content-type="genus-species">Staphylococcus aureus</named-content>

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