Pseudoalteromone A, a Ubiquinone Derivative from Marine Pseudoalteromonas spp., Suppresses Melanogenesis

An ubiquinone derivative, pseudoalteromone A (1), has been isolated from two marine-derived Pseudoalteromonas spp., APmarine002 and ROA-050, and its anti-melanogenesis activity was investigated. The anti-melanogenic capacity of pseudoalteromone A was demonstrate...

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Autores principales:	Su-Jin Lim, Dae-jin Min, Sohee Kim, Jihye Lee, Eun-Soo Lee, Hyuk Kim, Sung-Yoen Cho, Heung-Soo Beak, Chang-Seok Lee, Sang-Jip Nam, Jaeyoung Ko
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	<i>Pseudoalteromonas</i> sp. pseudoalteromone A marine natural product melanogenesis Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/0dcd7094f8094cfa90c6140ff49be417
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spelling	oai:doaj.org-article:0dcd7094f8094cfa90c6140ff49be4172021-11-25T18:12:49ZPseudoalteromone A, a Ubiquinone Derivative from Marine <i>Pseudoalteromonas</i> spp., Suppresses Melanogenesis10.3390/md191106121660-3397https://doaj.org/article/0dcd7094f8094cfa90c6140ff49be4172021-10-01T00:00:00Zhttps://www.mdpi.com/1660-3397/19/11/612https://doaj.org/toc/1660-3397An ubiquinone derivative, pseudoalteromone A (<b>1</b>), has been isolated from two marine-derived <i>Pseudoalteromonas</i> spp., APmarine002 and ROA-050, and its anti-melanogenesis activity was investigated. The anti-melanogenic capacity of pseudoalteromone A was demonstrated by assessing the intracellular and extracellular melanin content and cellular tyrosinase activity in the B16 cell line, Melan-a mouse melanocyte cell line, and MNT-1 human malignant melanoma cell line. Treatment with pseudoalteromone A (40 μg/mL) for 72 h reduced α-melanocyte-stimulating hormone (α-MSH)-induced intracellular melanin production by up to 44.68% in B16 cells and 38.24% in MNT-1 cells. Notably, pseudoalteromone A induced a concentration-dependent reduction in cellular tyrosinase activity in B16 cell, and Western blot analyses showed that this inhibitory activity was associated with a significant decrease in protein levels of tyrosinase and tyrosinase-related protein 1 (Tyrp-1), suggesting that pseudoalteromone A exerts its anti-melanogenesis activity through effects on melanogenic genes. We further evaluated the skin-whitening effect of pseudoalteromone A in the three-dimensional (3D) pigmented-epidermis model, MelanoDerm, and visualized the 3D distribution of melanin by two-photon excited fluorescence imaging in this human skin equivalent. Collectively, our findings suggest that pseudoalteromone A inhibits tyrosinase activity and expression and that this accounts for its anti-melanogenic effects in melanocytes.Su-Jin LimDae-jin MinSohee KimJihye LeeEun-Soo LeeHyuk KimSung-Yoen ChoHeung-Soo BeakChang-Seok LeeSang-Jip NamJaeyoung KoMDPI AGarticle<i>Pseudoalteromonas</i> sp.pseudoalteromone Amarine natural productmelanogenesisBiology (General)QH301-705.5ENMarine Drugs, Vol 19, Iss 612, p 612 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	<i>Pseudoalteromonas</i> sp. pseudoalteromone A marine natural product melanogenesis Biology (General) QH301-705.5
spellingShingle	<i>Pseudoalteromonas</i> sp. pseudoalteromone A marine natural product melanogenesis Biology (General) QH301-705.5 Su-Jin Lim Dae-jin Min Sohee Kim Jihye Lee Eun-Soo Lee Hyuk Kim Sung-Yoen Cho Heung-Soo Beak Chang-Seok Lee Sang-Jip Nam Jaeyoung Ko Pseudoalteromone A, a Ubiquinone Derivative from Marine <i>Pseudoalteromonas</i> spp., Suppresses Melanogenesis
description	An ubiquinone derivative, pseudoalteromone A (<b>1</b>), has been isolated from two marine-derived <i>Pseudoalteromonas</i> spp., APmarine002 and ROA-050, and its anti-melanogenesis activity was investigated. The anti-melanogenic capacity of pseudoalteromone A was demonstrated by assessing the intracellular and extracellular melanin content and cellular tyrosinase activity in the B16 cell line, Melan-a mouse melanocyte cell line, and MNT-1 human malignant melanoma cell line. Treatment with pseudoalteromone A (40 μg/mL) for 72 h reduced α-melanocyte-stimulating hormone (α-MSH)-induced intracellular melanin production by up to 44.68% in B16 cells and 38.24% in MNT-1 cells. Notably, pseudoalteromone A induced a concentration-dependent reduction in cellular tyrosinase activity in B16 cell, and Western blot analyses showed that this inhibitory activity was associated with a significant decrease in protein levels of tyrosinase and tyrosinase-related protein 1 (Tyrp-1), suggesting that pseudoalteromone A exerts its anti-melanogenesis activity through effects on melanogenic genes. We further evaluated the skin-whitening effect of pseudoalteromone A in the three-dimensional (3D) pigmented-epidermis model, MelanoDerm, and visualized the 3D distribution of melanin by two-photon excited fluorescence imaging in this human skin equivalent. Collectively, our findings suggest that pseudoalteromone A inhibits tyrosinase activity and expression and that this accounts for its anti-melanogenic effects in melanocytes.
format	article
author	Su-Jin Lim Dae-jin Min Sohee Kim Jihye Lee Eun-Soo Lee Hyuk Kim Sung-Yoen Cho Heung-Soo Beak Chang-Seok Lee Sang-Jip Nam Jaeyoung Ko
author_facet	Su-Jin Lim Dae-jin Min Sohee Kim Jihye Lee Eun-Soo Lee Hyuk Kim Sung-Yoen Cho Heung-Soo Beak Chang-Seok Lee Sang-Jip Nam Jaeyoung Ko
author_sort	Su-Jin Lim
title	Pseudoalteromone A, a Ubiquinone Derivative from Marine <i>Pseudoalteromonas</i> spp., Suppresses Melanogenesis
title_short	Pseudoalteromone A, a Ubiquinone Derivative from Marine <i>Pseudoalteromonas</i> spp., Suppresses Melanogenesis
title_full	Pseudoalteromone A, a Ubiquinone Derivative from Marine <i>Pseudoalteromonas</i> spp., Suppresses Melanogenesis
title_fullStr	Pseudoalteromone A, a Ubiquinone Derivative from Marine <i>Pseudoalteromonas</i> spp., Suppresses Melanogenesis
title_full_unstemmed	Pseudoalteromone A, a Ubiquinone Derivative from Marine <i>Pseudoalteromonas</i> spp., Suppresses Melanogenesis
title_sort	pseudoalteromone a, a ubiquinone derivative from marine <i>pseudoalteromonas</i> spp., suppresses melanogenesis
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/0dcd7094f8094cfa90c6140ff49be417
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Pseudoalteromone A, a Ubiquinone Derivative from Marine <i>Pseudoalteromonas</i> spp., Suppresses Melanogenesis

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