Predicting blooms of toxic cyanobacteria in eutrophic lakes with diverse cyanobacterial communities

Abstract We investigated possibility of predicting whether blooms, if they occur, would be formed of microcystin-producing cyanobacteria. DGGE analysis of 16S-ITS and mcyA genes revealed that only Planktothrix and Microcystis possessed mcy-genes and Planktothrix was the main microcystin producer. qP...

Descripción completa

Guardado en:
Detalles Bibliográficos
Autores principales: Aleksandra Bukowska, Tomasz Kaliński, Michał Koper, Iwona Kostrzewska-Szlakowska, Jan Kwiatowski, Hanna Mazur-Marzec, Iwona Jasser
Formato: article
Lenguaje:EN
Publicado: Nature Portfolio 2017
Materias:
R
Q
Acceso en línea:https://doaj.org/article/0dcea9ef70b0424b86fcb4c216173ec3
Etiquetas: Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
id oai:doaj.org-article:0dcea9ef70b0424b86fcb4c216173ec3
record_format dspace
spelling oai:doaj.org-article:0dcea9ef70b0424b86fcb4c216173ec32021-12-02T11:41:00ZPredicting blooms of toxic cyanobacteria in eutrophic lakes with diverse cyanobacterial communities10.1038/s41598-017-08701-82045-2322https://doaj.org/article/0dcea9ef70b0424b86fcb4c216173ec32017-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-08701-8https://doaj.org/toc/2045-2322Abstract We investigated possibility of predicting whether blooms, if they occur, would be formed of microcystin-producing cyanobacteria. DGGE analysis of 16S-ITS and mcyA genes revealed that only Planktothrix and Microcystis possessed mcy-genes and Planktothrix was the main microcystin producer. qPCR analysis revealed that the proportion of cells with mcy-genes in Planktothrix populations was almost 100%. Microcystin concentration correlated with the number of potentially toxic and total Planktothrix cells and the proportion of Planktothrix within all cyanobacteria, but not with the proportion of cells with mcy-genes in total Planktothrix. The share of Microcystis cells with mcy-genes was low and variable in time. Neither the number of mcy-possessing cells, nor the proportion of these cells in total Microcystis, correlated with the concentration of microcystins. This suggests that it is possible to predict whether the bloom in the Masurian Lakes will be toxic based on Planktothrix occurrence. Two species of toxin producing Planktothrix, P. agardhii and P. rubescens, were identified by phylogenetic analysis of 16S-ITS. Based on morphological and ecological features, the toxic Planktothrix was identified as P. agardhii. However, the very high proportion of cells with mcy-genes suggests P. rubescens. Our study reveals the need of universal primers for mcyA genes from environment.Aleksandra BukowskaTomasz KalińskiMichał KoperIwona Kostrzewska-SzlakowskaJan KwiatowskiHanna Mazur-MarzecIwona JasserNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-12 (2017)
institution DOAJ
collection DOAJ
language EN
topic Medicine
R
Science
Q
spellingShingle Medicine
R
Science
Q
Aleksandra Bukowska
Tomasz Kaliński
Michał Koper
Iwona Kostrzewska-Szlakowska
Jan Kwiatowski
Hanna Mazur-Marzec
Iwona Jasser
Predicting blooms of toxic cyanobacteria in eutrophic lakes with diverse cyanobacterial communities
description Abstract We investigated possibility of predicting whether blooms, if they occur, would be formed of microcystin-producing cyanobacteria. DGGE analysis of 16S-ITS and mcyA genes revealed that only Planktothrix and Microcystis possessed mcy-genes and Planktothrix was the main microcystin producer. qPCR analysis revealed that the proportion of cells with mcy-genes in Planktothrix populations was almost 100%. Microcystin concentration correlated with the number of potentially toxic and total Planktothrix cells and the proportion of Planktothrix within all cyanobacteria, but not with the proportion of cells with mcy-genes in total Planktothrix. The share of Microcystis cells with mcy-genes was low and variable in time. Neither the number of mcy-possessing cells, nor the proportion of these cells in total Microcystis, correlated with the concentration of microcystins. This suggests that it is possible to predict whether the bloom in the Masurian Lakes will be toxic based on Planktothrix occurrence. Two species of toxin producing Planktothrix, P. agardhii and P. rubescens, were identified by phylogenetic analysis of 16S-ITS. Based on morphological and ecological features, the toxic Planktothrix was identified as P. agardhii. However, the very high proportion of cells with mcy-genes suggests P. rubescens. Our study reveals the need of universal primers for mcyA genes from environment.
format article
author Aleksandra Bukowska
Tomasz Kaliński
Michał Koper
Iwona Kostrzewska-Szlakowska
Jan Kwiatowski
Hanna Mazur-Marzec
Iwona Jasser
author_facet Aleksandra Bukowska
Tomasz Kaliński
Michał Koper
Iwona Kostrzewska-Szlakowska
Jan Kwiatowski
Hanna Mazur-Marzec
Iwona Jasser
author_sort Aleksandra Bukowska
title Predicting blooms of toxic cyanobacteria in eutrophic lakes with diverse cyanobacterial communities
title_short Predicting blooms of toxic cyanobacteria in eutrophic lakes with diverse cyanobacterial communities
title_full Predicting blooms of toxic cyanobacteria in eutrophic lakes with diverse cyanobacterial communities
title_fullStr Predicting blooms of toxic cyanobacteria in eutrophic lakes with diverse cyanobacterial communities
title_full_unstemmed Predicting blooms of toxic cyanobacteria in eutrophic lakes with diverse cyanobacterial communities
title_sort predicting blooms of toxic cyanobacteria in eutrophic lakes with diverse cyanobacterial communities
publisher Nature Portfolio
publishDate 2017
url https://doaj.org/article/0dcea9ef70b0424b86fcb4c216173ec3
work_keys_str_mv AT aleksandrabukowska predictingbloomsoftoxiccyanobacteriaineutrophiclakeswithdiversecyanobacterialcommunities
AT tomaszkalinski predictingbloomsoftoxiccyanobacteriaineutrophiclakeswithdiversecyanobacterialcommunities
AT michałkoper predictingbloomsoftoxiccyanobacteriaineutrophiclakeswithdiversecyanobacterialcommunities
AT iwonakostrzewskaszlakowska predictingbloomsoftoxiccyanobacteriaineutrophiclakeswithdiversecyanobacterialcommunities
AT jankwiatowski predictingbloomsoftoxiccyanobacteriaineutrophiclakeswithdiversecyanobacterialcommunities
AT hannamazurmarzec predictingbloomsoftoxiccyanobacteriaineutrophiclakeswithdiversecyanobacterialcommunities
AT iwonajasser predictingbloomsoftoxiccyanobacteriaineutrophiclakeswithdiversecyanobacterialcommunities
_version_ 1718395444088799232