Functionality of the Paracoccidioides mating α-pheromone-receptor system.

Recent evidence suggests that Paracoccidioides species have the potential to undergo sexual reproduction, although no sexual cycle has been identified either in nature or under laboratory conditions. In the present work we detected low expression levels of the heterothallic MAT loci genes MAT1-1 and...

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Autores principales:	Jéssica A Gomes-Rezende, Ana G Gomes-Alves, João F Menino, Marco A Coelho, Paula Ludovico, Paula Gonçalves, Mark H J Sturme, Fernando Rodrigues
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/0dcf0fdecf8f4beb94ee29154bc548e0
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spelling	oai:doaj.org-article:0dcf0fdecf8f4beb94ee29154bc548e02021-11-18T08:13:09ZFunctionality of the Paracoccidioides mating α-pheromone-receptor system.1932-620310.1371/journal.pone.0047033https://doaj.org/article/0dcf0fdecf8f4beb94ee29154bc548e02012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23056569/?tool=EBIhttps://doaj.org/toc/1932-6203Recent evidence suggests that Paracoccidioides species have the potential to undergo sexual reproduction, although no sexual cycle has been identified either in nature or under laboratory conditions. In the present work we detected low expression levels of the heterothallic MAT loci genes MAT1-1 and MAT1-2, the α-pheromone (PBα) gene, and the α- and a-pheromone receptor (PREB and PREA) genes in yeast and mycelia forms of several Paracoccidioides isolates. None of the genes were expressed in a mating type dependent manner. Stimulation of P. brasiliensis MAT1-2 strains with the synthetic α-pheromone peptide failed to elicit transcriptional activation of MAT1-2, PREB or STE12, suggesting that the strains tested are insensitive to α-pheromone. In order to further evaluate the biological functionality of the pair α-pheromone and its receptor, we took advantage of the heterologous expression of these Paracoccidioides genes in the corresponding S. cerevisiae null mutants. We show that S. cerevisiae strains heterologously expressing PREB respond to Pbα pheromone either isolated from Paracoccidioides culture supernatants or in its synthetic form, both by shmoo formation and by growth and cell cycle arrests. This allowed us to conclude that Paracoccidioides species secrete an active α-pheromone into the culture medium that is able to activate its cognate receptor. Moreover, expression of PREB or PBα in the corresponding null mutants of S. cerevisiae restored mating in these non-fertile strains. Taken together, our data demonstrate pheromone signaling activation by the Paracoccidioides α-pheromone through its receptor in this yeast model, which provides novel evidence for the existence of a functional mating signaling system in Paracoccidioides.Jéssica A Gomes-RezendeAna G Gomes-AlvesJoão F MeninoMarco A CoelhoPaula LudovicoPaula GonçalvesMark H J SturmeFernando RodriguesPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 10, p e47033 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Jéssica A Gomes-Rezende Ana G Gomes-Alves João F Menino Marco A Coelho Paula Ludovico Paula Gonçalves Mark H J Sturme Fernando Rodrigues Functionality of the Paracoccidioides mating α-pheromone-receptor system.
description	Recent evidence suggests that Paracoccidioides species have the potential to undergo sexual reproduction, although no sexual cycle has been identified either in nature or under laboratory conditions. In the present work we detected low expression levels of the heterothallic MAT loci genes MAT1-1 and MAT1-2, the α-pheromone (PBα) gene, and the α- and a-pheromone receptor (PREB and PREA) genes in yeast and mycelia forms of several Paracoccidioides isolates. None of the genes were expressed in a mating type dependent manner. Stimulation of P. brasiliensis MAT1-2 strains with the synthetic α-pheromone peptide failed to elicit transcriptional activation of MAT1-2, PREB or STE12, suggesting that the strains tested are insensitive to α-pheromone. In order to further evaluate the biological functionality of the pair α-pheromone and its receptor, we took advantage of the heterologous expression of these Paracoccidioides genes in the corresponding S. cerevisiae null mutants. We show that S. cerevisiae strains heterologously expressing PREB respond to Pbα pheromone either isolated from Paracoccidioides culture supernatants or in its synthetic form, both by shmoo formation and by growth and cell cycle arrests. This allowed us to conclude that Paracoccidioides species secrete an active α-pheromone into the culture medium that is able to activate its cognate receptor. Moreover, expression of PREB or PBα in the corresponding null mutants of S. cerevisiae restored mating in these non-fertile strains. Taken together, our data demonstrate pheromone signaling activation by the Paracoccidioides α-pheromone through its receptor in this yeast model, which provides novel evidence for the existence of a functional mating signaling system in Paracoccidioides.
format	article
author	Jéssica A Gomes-Rezende Ana G Gomes-Alves João F Menino Marco A Coelho Paula Ludovico Paula Gonçalves Mark H J Sturme Fernando Rodrigues
author_facet	Jéssica A Gomes-Rezende Ana G Gomes-Alves João F Menino Marco A Coelho Paula Ludovico Paula Gonçalves Mark H J Sturme Fernando Rodrigues
author_sort	Jéssica A Gomes-Rezende
title	Functionality of the Paracoccidioides mating α-pheromone-receptor system.
title_short	Functionality of the Paracoccidioides mating α-pheromone-receptor system.
title_full	Functionality of the Paracoccidioides mating α-pheromone-receptor system.
title_fullStr	Functionality of the Paracoccidioides mating α-pheromone-receptor system.
title_full_unstemmed	Functionality of the Paracoccidioides mating α-pheromone-receptor system.
title_sort	functionality of the paracoccidioides mating α-pheromone-receptor system.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/0dcf0fdecf8f4beb94ee29154bc548e0
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Functionality of the Paracoccidioides mating α-pheromone-receptor system.

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