Co-culture of type I and type II pneumocytes as a model of alveolar epithelium.

The epithelial tissues of the distal lung are continuously exposed to inhaled air, and are of research interest in studying respiratory exposure to both hazardous and therapeutic materials. Pharmaco-toxicological research depends on the development of sophisticated models of the alveolar epithelium,...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Oliver Brookes, Sonja Boland, René Lai Kuen, Dorian Miremont, Jamileh Movassat, Armelle Baeza-Squiban
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/0dcfca0274cc4c439984bef6fb9ac23b
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:0dcfca0274cc4c439984bef6fb9ac23b
record_format	dspace
spelling	oai:doaj.org-article:0dcfca0274cc4c439984bef6fb9ac23b2021-12-02T20:07:59ZCo-culture of type I and type II pneumocytes as a model of alveolar epithelium.1932-620310.1371/journal.pone.0248798https://doaj.org/article/0dcfca0274cc4c439984bef6fb9ac23b2021-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0248798https://doaj.org/toc/1932-6203The epithelial tissues of the distal lung are continuously exposed to inhaled air, and are of research interest in studying respiratory exposure to both hazardous and therapeutic materials. Pharmaco-toxicological research depends on the development of sophisticated models of the alveolar epithelium, which better represent the different cell types present in the native lung and interactions between them. We developed an air-liquid interface (ALI) model of the alveolar epithelium which incorporates cell lines which bear features of type I (hAELVi) and type II (NCI-H441) epithelial cells. We compared morphology of single cells and the structure of cell layers of the two lines using light and electron microscopy. Working both in monotypic cultures and cocultures, we measured barrier function by trans-epithelial electrical resistance (TEER), and demonstrated that barrier properties can be maintained for 30 days. We created a mathematical model of TEER development over time based on these data in order to make inferences about the interactions occurring in these culture systems. We assessed expression of a panel of relevant genes that play important roles in barrier function and differentiation. The coculture model was observed to form a stable barrier akin to that seen in hAELVi, while expressing surfactant protein C, and having a profile of expression of claudins and aquaporins appropriate for the distal lung. We described cavities which arise within stratified cell layers in NCI-H441 and cocultured cells, and present evidence that these cavities represent an aberrant apical surface. In summary, our results support the coculture of these two cell lines to produce a model which better represents the breadth of functions seen in native alveolar epithelium.Oliver BrookesSonja BolandRené Lai KuenDorian MiremontJamileh MovassatArmelle Baeza-SquibanPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 9, p e0248798 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Oliver Brookes Sonja Boland René Lai Kuen Dorian Miremont Jamileh Movassat Armelle Baeza-Squiban Co-culture of type I and type II pneumocytes as a model of alveolar epithelium.
description	The epithelial tissues of the distal lung are continuously exposed to inhaled air, and are of research interest in studying respiratory exposure to both hazardous and therapeutic materials. Pharmaco-toxicological research depends on the development of sophisticated models of the alveolar epithelium, which better represent the different cell types present in the native lung and interactions between them. We developed an air-liquid interface (ALI) model of the alveolar epithelium which incorporates cell lines which bear features of type I (hAELVi) and type II (NCI-H441) epithelial cells. We compared morphology of single cells and the structure of cell layers of the two lines using light and electron microscopy. Working both in monotypic cultures and cocultures, we measured barrier function by trans-epithelial electrical resistance (TEER), and demonstrated that barrier properties can be maintained for 30 days. We created a mathematical model of TEER development over time based on these data in order to make inferences about the interactions occurring in these culture systems. We assessed expression of a panel of relevant genes that play important roles in barrier function and differentiation. The coculture model was observed to form a stable barrier akin to that seen in hAELVi, while expressing surfactant protein C, and having a profile of expression of claudins and aquaporins appropriate for the distal lung. We described cavities which arise within stratified cell layers in NCI-H441 and cocultured cells, and present evidence that these cavities represent an aberrant apical surface. In summary, our results support the coculture of these two cell lines to produce a model which better represents the breadth of functions seen in native alveolar epithelium.
format	article
author	Oliver Brookes Sonja Boland René Lai Kuen Dorian Miremont Jamileh Movassat Armelle Baeza-Squiban
author_facet	Oliver Brookes Sonja Boland René Lai Kuen Dorian Miremont Jamileh Movassat Armelle Baeza-Squiban
author_sort	Oliver Brookes
title	Co-culture of type I and type II pneumocytes as a model of alveolar epithelium.
title_short	Co-culture of type I and type II pneumocytes as a model of alveolar epithelium.
title_full	Co-culture of type I and type II pneumocytes as a model of alveolar epithelium.
title_fullStr	Co-culture of type I and type II pneumocytes as a model of alveolar epithelium.
title_full_unstemmed	Co-culture of type I and type II pneumocytes as a model of alveolar epithelium.
title_sort	co-culture of type i and type ii pneumocytes as a model of alveolar epithelium.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/0dcfca0274cc4c439984bef6fb9ac23b
work_keys_str_mv	AT oliverbrookes cocultureoftypeiandtypeiipneumocytesasamodelofalveolarepithelium AT sonjaboland cocultureoftypeiandtypeiipneumocytesasamodelofalveolarepithelium AT renelaikuen cocultureoftypeiandtypeiipneumocytesasamodelofalveolarepithelium AT dorianmiremont cocultureoftypeiandtypeiipneumocytesasamodelofalveolarepithelium AT jamilehmovassat cocultureoftypeiandtypeiipneumocytesasamodelofalveolarepithelium AT armellebaezasquiban cocultureoftypeiandtypeiipneumocytesasamodelofalveolarepithelium
_version_	1718375224814075904

Co-culture of type I and type II pneumocytes as a model of alveolar epithelium.

Ejemplares similares