The establishment of an immunosensor for the detection of SPOP

Abstract In this paper, we first synthesis three-dimensional jasmine-like Cu@L-aspartic acid(L-ASP) inorganic–organic hybrid nanoflowers to load palladium-platinum nanoparticles (Pd–Pt NPs) as the signal enhancer in order to quantify intracellular speckle-type POZ domain protein. Scanning electron m...

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Autores principales:	Song Yue, Kexin Sun, Siyuan Li, Yi Liu, Qihao Zhu, Yiyu Chen, Dong Yuan, Tao Wen, Mingjian Ge, Qiubo Yu
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/0dd6133128be4aaf8a664c301bc5be2b
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spelling	oai:doaj.org-article:0dd6133128be4aaf8a664c301bc5be2b2021-12-02T16:04:27ZThe establishment of an immunosensor for the detection of SPOP10.1038/s41598-021-91944-32045-2322https://doaj.org/article/0dd6133128be4aaf8a664c301bc5be2b2021-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-91944-3https://doaj.org/toc/2045-2322Abstract In this paper, we first synthesis three-dimensional jasmine-like Cu@L-aspartic acid(L-ASP) inorganic–organic hybrid nanoflowers to load palladium-platinum nanoparticles (Pd–Pt NPs) as the signal enhancer in order to quantify intracellular speckle-type POZ domain protein. Scanning electron microscope, fourier transform infrared, energy dispersive spectrometer, X-ray photoelectron spectroscopy analysis was used to characterize the newly synthesized materials. The newly formed Cu@L-Asp/Pd-PtNPs can catalyze the decomposition of hydrogen peroxide and exhibit excellent catalytic performance. When different concentration of speckle-type POZ domain protein is captured by speckle-type POZ domain protein antibody linked to the surface of Cu@L-Asp/Pd–Pt NPs, the current signal decreases with the increase concentration of speckle-type POZ domain protein. After optimization, the speckle-type POZ domain protein immunosensor exhibited a good linear response over a concentration range from 0.1–1 ng mL−1 with a low detection limit of 19 fg mL−1. The proposed sensor demonstrates good stability within 28 days, acceptable reproducibility (RSD = 0.52%) and selectivity to the speckle-type POZ domain protein in the presence of possible interfering substances and has potential application for detecting other intracellular macromolecular substances.Song YueKexin SunSiyuan LiYi LiuQihao ZhuYiyu ChenDong YuanTao WenMingjian GeQiubo YuNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-9 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Song Yue Kexin Sun Siyuan Li Yi Liu Qihao Zhu Yiyu Chen Dong Yuan Tao Wen Mingjian Ge Qiubo Yu The establishment of an immunosensor for the detection of SPOP
description	Abstract In this paper, we first synthesis three-dimensional jasmine-like Cu@L-aspartic acid(L-ASP) inorganic–organic hybrid nanoflowers to load palladium-platinum nanoparticles (Pd–Pt NPs) as the signal enhancer in order to quantify intracellular speckle-type POZ domain protein. Scanning electron microscope, fourier transform infrared, energy dispersive spectrometer, X-ray photoelectron spectroscopy analysis was used to characterize the newly synthesized materials. The newly formed Cu@L-Asp/Pd-PtNPs can catalyze the decomposition of hydrogen peroxide and exhibit excellent catalytic performance. When different concentration of speckle-type POZ domain protein is captured by speckle-type POZ domain protein antibody linked to the surface of Cu@L-Asp/Pd–Pt NPs, the current signal decreases with the increase concentration of speckle-type POZ domain protein. After optimization, the speckle-type POZ domain protein immunosensor exhibited a good linear response over a concentration range from 0.1–1 ng mL−1 with a low detection limit of 19 fg mL−1. The proposed sensor demonstrates good stability within 28 days, acceptable reproducibility (RSD = 0.52%) and selectivity to the speckle-type POZ domain protein in the presence of possible interfering substances and has potential application for detecting other intracellular macromolecular substances.
format	article
author	Song Yue Kexin Sun Siyuan Li Yi Liu Qihao Zhu Yiyu Chen Dong Yuan Tao Wen Mingjian Ge Qiubo Yu
author_facet	Song Yue Kexin Sun Siyuan Li Yi Liu Qihao Zhu Yiyu Chen Dong Yuan Tao Wen Mingjian Ge Qiubo Yu
author_sort	Song Yue
title	The establishment of an immunosensor for the detection of SPOP
title_short	The establishment of an immunosensor for the detection of SPOP
title_full	The establishment of an immunosensor for the detection of SPOP
title_fullStr	The establishment of an immunosensor for the detection of SPOP
title_full_unstemmed	The establishment of an immunosensor for the detection of SPOP
title_sort	establishment of an immunosensor for the detection of spop
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/0dd6133128be4aaf8a664c301bc5be2b
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The establishment of an immunosensor for the detection of SPOP

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