Sequencing and characterization of an L-asparaginase gene from a new species of Penicillium section Citrina isolated from Cerrado

Abstract The enzyme L-asparaginase (L-ASNase) is used in the treatment of Acute Lymphoblastic Leukemia. The preparations of this enzyme for clinical use are derived from bacterial sources and its use is associated with serious adverse reactions. In this context, it is important to find new sources o...

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Autores principales:	Kellen C. R. Andrade, Rildo A. Fernandes, Danilo Batista Pinho, Marcela M. de Freitas, Edivaldo Ximenes Ferreira Filho, Adalberto Pessoa, João Inácio Silva, Perola O. Magalhães
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/0ddc797cf0064351ac855f8c8ee61da4
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spelling	oai:doaj.org-article:0ddc797cf0064351ac855f8c8ee61da42021-12-02T19:12:25ZSequencing and characterization of an L-asparaginase gene from a new species of Penicillium section Citrina isolated from Cerrado10.1038/s41598-021-97316-12045-2322https://doaj.org/article/0ddc797cf0064351ac855f8c8ee61da42021-09-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-97316-1https://doaj.org/toc/2045-2322Abstract The enzyme L-asparaginase (L-ASNase) is used in the treatment of Acute Lymphoblastic Leukemia. The preparations of this enzyme for clinical use are derived from bacterial sources and its use is associated with serious adverse reactions. In this context, it is important to find new sources of L-ASNase. In this work, the Placket-Burman Experimental Design (PBD) was used to determine the influence of the variables on the L-ASNase production then it was followed by a 28–4 Factorial Fractional Design (FFD). The results obtained from PBD have shown a range of L-ASNase activity, from 0.47 to 1.77 U/gcell and the results obtained from FFD have showed a range of L-ASNase activity, from 1.10 to 2.36 U/gcell. L-proline and ammonium sulfate were identified as of significant positive variables on this production enzyme by Penicillium cerradense sp. nov. The precise identification of this new species was confirmed by morphological characteristics and sequence comparisons of the nuclear 18S-5.8S-28S partial nrDNA including the ITS1 and ITS2 regions, RNA polymerase II, β-tubulin and calmodulin genomic regions. The genetic sequence coding for the L-ASNase was obtained after carrying out a full genome sequencing. The L-ASNase expressed by P. cerradense sp. nov may have promising antineoplastic properties.Kellen C. R. AndradeRildo A. FernandesDanilo Batista PinhoMarcela M. de FreitasEdivaldo Ximenes Ferreira FilhoAdalberto PessoaJoão Inácio SilvaPerola O. MagalhãesNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-13 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Kellen C. R. Andrade Rildo A. Fernandes Danilo Batista Pinho Marcela M. de Freitas Edivaldo Ximenes Ferreira Filho Adalberto Pessoa João Inácio Silva Perola O. Magalhães Sequencing and characterization of an L-asparaginase gene from a new species of Penicillium section Citrina isolated from Cerrado
description	Abstract The enzyme L-asparaginase (L-ASNase) is used in the treatment of Acute Lymphoblastic Leukemia. The preparations of this enzyme for clinical use are derived from bacterial sources and its use is associated with serious adverse reactions. In this context, it is important to find new sources of L-ASNase. In this work, the Placket-Burman Experimental Design (PBD) was used to determine the influence of the variables on the L-ASNase production then it was followed by a 28–4 Factorial Fractional Design (FFD). The results obtained from PBD have shown a range of L-ASNase activity, from 0.47 to 1.77 U/gcell and the results obtained from FFD have showed a range of L-ASNase activity, from 1.10 to 2.36 U/gcell. L-proline and ammonium sulfate were identified as of significant positive variables on this production enzyme by Penicillium cerradense sp. nov. The precise identification of this new species was confirmed by morphological characteristics and sequence comparisons of the nuclear 18S-5.8S-28S partial nrDNA including the ITS1 and ITS2 regions, RNA polymerase II, β-tubulin and calmodulin genomic regions. The genetic sequence coding for the L-ASNase was obtained after carrying out a full genome sequencing. The L-ASNase expressed by P. cerradense sp. nov may have promising antineoplastic properties.
format	article
author	Kellen C. R. Andrade Rildo A. Fernandes Danilo Batista Pinho Marcela M. de Freitas Edivaldo Ximenes Ferreira Filho Adalberto Pessoa João Inácio Silva Perola O. Magalhães
author_facet	Kellen C. R. Andrade Rildo A. Fernandes Danilo Batista Pinho Marcela M. de Freitas Edivaldo Ximenes Ferreira Filho Adalberto Pessoa João Inácio Silva Perola O. Magalhães
author_sort	Kellen C. R. Andrade
title	Sequencing and characterization of an L-asparaginase gene from a new species of Penicillium section Citrina isolated from Cerrado
title_short	Sequencing and characterization of an L-asparaginase gene from a new species of Penicillium section Citrina isolated from Cerrado
title_full	Sequencing and characterization of an L-asparaginase gene from a new species of Penicillium section Citrina isolated from Cerrado
title_fullStr	Sequencing and characterization of an L-asparaginase gene from a new species of Penicillium section Citrina isolated from Cerrado
title_full_unstemmed	Sequencing and characterization of an L-asparaginase gene from a new species of Penicillium section Citrina isolated from Cerrado
title_sort	sequencing and characterization of an l-asparaginase gene from a new species of penicillium section citrina isolated from cerrado
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/0ddc797cf0064351ac855f8c8ee61da4
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Sequencing and characterization of an L-asparaginase gene from a new species of Penicillium section Citrina isolated from Cerrado

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