MYC-type transcription factors, MYC67 and MYC70, interact with ICE1 and negatively regulate cold tolerance in Arabidopsis

Abstract The expression of hundreds of genes is induced by low temperatures via a cold signaling pathway. ICE1, a MYC-type transcription factor, plays an important role in the induction of CBF3/DREB1A to control cold-responsive genes and cold tolerance. To elucidate other molecular factors, a yeast...

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Autores principales:	Masaru Ohta, Aiko Sato, Na Renhu, Tsuyoshi Yamamoto, Nodoka Oka, Jian-Kang Zhu, Yasuomi Tada, Takuya Suzaki, Kenji Miura
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/0ddeb95edcda4867a96f5e57cd83f1ab
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spelling	oai:doaj.org-article:0ddeb95edcda4867a96f5e57cd83f1ab2021-12-02T15:08:19ZMYC-type transcription factors, MYC67 and MYC70, interact with ICE1 and negatively regulate cold tolerance in Arabidopsis10.1038/s41598-018-29722-x2045-2322https://doaj.org/article/0ddeb95edcda4867a96f5e57cd83f1ab2018-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-29722-xhttps://doaj.org/toc/2045-2322Abstract The expression of hundreds of genes is induced by low temperatures via a cold signaling pathway. ICE1, a MYC-type transcription factor, plays an important role in the induction of CBF3/DREB1A to control cold-responsive genes and cold tolerance. To elucidate other molecular factors, a yeast 2-hybrid screening was performed. Two MYC-type transcription factors, MYC67 and MYC70, were identified as ICE1-interacting proteins. The myc mutants were more tolerant to freezing temperatures than wild type. CBF3/DREB1A and other cold-responsive genes were up-regulated in the myc mutants. Overexpression of the MYC genes increased the cold sensitivity and down-regulated the expression of cold-responsive genes. The MYC proteins interacted with the cis-elements in the CBF3/DREB1A promoter, probably to interfere interaction between ICE1 and the cis-elements. Taken together, these results demonstrate that MYC67 and MYC70, ICE1 interactors, negatively regulate cold-responsive genes and cold tolerance.Masaru OhtaAiko SatoNa RenhuTsuyoshi YamamotoNodoka OkaJian-Kang ZhuYasuomi TadaTakuya SuzakiKenji MiuraNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-12 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Masaru Ohta Aiko Sato Na Renhu Tsuyoshi Yamamoto Nodoka Oka Jian-Kang Zhu Yasuomi Tada Takuya Suzaki Kenji Miura MYC-type transcription factors, MYC67 and MYC70, interact with ICE1 and negatively regulate cold tolerance in Arabidopsis
description	Abstract The expression of hundreds of genes is induced by low temperatures via a cold signaling pathway. ICE1, a MYC-type transcription factor, plays an important role in the induction of CBF3/DREB1A to control cold-responsive genes and cold tolerance. To elucidate other molecular factors, a yeast 2-hybrid screening was performed. Two MYC-type transcription factors, MYC67 and MYC70, were identified as ICE1-interacting proteins. The myc mutants were more tolerant to freezing temperatures than wild type. CBF3/DREB1A and other cold-responsive genes were up-regulated in the myc mutants. Overexpression of the MYC genes increased the cold sensitivity and down-regulated the expression of cold-responsive genes. The MYC proteins interacted with the cis-elements in the CBF3/DREB1A promoter, probably to interfere interaction between ICE1 and the cis-elements. Taken together, these results demonstrate that MYC67 and MYC70, ICE1 interactors, negatively regulate cold-responsive genes and cold tolerance.
format	article
author	Masaru Ohta Aiko Sato Na Renhu Tsuyoshi Yamamoto Nodoka Oka Jian-Kang Zhu Yasuomi Tada Takuya Suzaki Kenji Miura
author_facet	Masaru Ohta Aiko Sato Na Renhu Tsuyoshi Yamamoto Nodoka Oka Jian-Kang Zhu Yasuomi Tada Takuya Suzaki Kenji Miura
author_sort	Masaru Ohta
title	MYC-type transcription factors, MYC67 and MYC70, interact with ICE1 and negatively regulate cold tolerance in Arabidopsis
title_short	MYC-type transcription factors, MYC67 and MYC70, interact with ICE1 and negatively regulate cold tolerance in Arabidopsis
title_full	MYC-type transcription factors, MYC67 and MYC70, interact with ICE1 and negatively regulate cold tolerance in Arabidopsis
title_fullStr	MYC-type transcription factors, MYC67 and MYC70, interact with ICE1 and negatively regulate cold tolerance in Arabidopsis
title_full_unstemmed	MYC-type transcription factors, MYC67 and MYC70, interact with ICE1 and negatively regulate cold tolerance in Arabidopsis
title_sort	myc-type transcription factors, myc67 and myc70, interact with ice1 and negatively regulate cold tolerance in arabidopsis
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/0ddeb95edcda4867a96f5e57cd83f1ab
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MYC-type transcription factors, MYC67 and MYC70, interact with ICE1 and negatively regulate cold tolerance in Arabidopsis

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