Transcriptomic analysis revealed increased expression of genes involved in keratinization in the tears of COVID-19 patients

Abstract Recent studies have focused their attention on conjunctivitis as one of the symptoms of coronavirus disease 2019 (COVID-19). Therefore, tear samples were taken from COVID-19 patients and the presence of SARS-CoV-2 was evidenced using Real Time reverse transcription polymerase chain reaction...

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Autores principales:	Leonardo Mastropasqua, Lisa Toto, Luigi Chiricosta, Francesca Diomede, Agnese Gugliandolo, Serena Silvestro, Guya Diletta Marconi, Bruna Sinjari, Jacopo Vecchiet, Francesco Cipollone, Damiano D’Ardes, Antonio Auricchio, Manuela Lanzini, Sergio Caputi, Rossella D’Aloisio, Emanuela Mazzon, Oriana Trubiani
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/0de353236beb40a9b301cdf412eaa96e
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spelling	oai:doaj.org-article:0de353236beb40a9b301cdf412eaa96e2021-12-02T18:37:08ZTranscriptomic analysis revealed increased expression of genes involved in keratinization in the tears of COVID-19 patients10.1038/s41598-021-99344-32045-2322https://doaj.org/article/0de353236beb40a9b301cdf412eaa96e2021-10-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-99344-3https://doaj.org/toc/2045-2322Abstract Recent studies have focused their attention on conjunctivitis as one of the symptoms of coronavirus disease 2019 (COVID-19). Therefore, tear samples were taken from COVID-19 patients and the presence of SARS-CoV-2 was evidenced using Real Time reverse transcription polymerase chain reaction. The main aim of this study was to analyze mRNA expression in the tears of patients with COVID-19 compared with healthy subjects using Next Generation Sequencing (NGS). The functional evaluation of the transcriptome highlighted 25 genes that differ statistically between healthy individuals and patients affected by COVID-19. In particular, the NGS analysis identified the presence of several genes involved in B cell signaling and keratinization. In particular, the genes involved in B cell signaling were downregulated in the tears of COVID-19 patients, while those involved in keratinization were upregulated. The results indicated that SARS-CoV-2 may induce a process of ocular keratinization and a defective B cell response.Leonardo MastropasquaLisa TotoLuigi ChiricostaFrancesca DiomedeAgnese GugliandoloSerena SilvestroGuya Diletta MarconiBruna SinjariJacopo VecchietFrancesco CipolloneDamiano D’ArdesAntonio AuricchioManuela LanziniSergio CaputiRossella D’AloisioEmanuela MazzonOriana TrubianiNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Leonardo Mastropasqua Lisa Toto Luigi Chiricosta Francesca Diomede Agnese Gugliandolo Serena Silvestro Guya Diletta Marconi Bruna Sinjari Jacopo Vecchiet Francesco Cipollone Damiano D’Ardes Antonio Auricchio Manuela Lanzini Sergio Caputi Rossella D’Aloisio Emanuela Mazzon Oriana Trubiani Transcriptomic analysis revealed increased expression of genes involved in keratinization in the tears of COVID-19 patients
description	Abstract Recent studies have focused their attention on conjunctivitis as one of the symptoms of coronavirus disease 2019 (COVID-19). Therefore, tear samples were taken from COVID-19 patients and the presence of SARS-CoV-2 was evidenced using Real Time reverse transcription polymerase chain reaction. The main aim of this study was to analyze mRNA expression in the tears of patients with COVID-19 compared with healthy subjects using Next Generation Sequencing (NGS). The functional evaluation of the transcriptome highlighted 25 genes that differ statistically between healthy individuals and patients affected by COVID-19. In particular, the NGS analysis identified the presence of several genes involved in B cell signaling and keratinization. In particular, the genes involved in B cell signaling were downregulated in the tears of COVID-19 patients, while those involved in keratinization were upregulated. The results indicated that SARS-CoV-2 may induce a process of ocular keratinization and a defective B cell response.
format	article
author	Leonardo Mastropasqua Lisa Toto Luigi Chiricosta Francesca Diomede Agnese Gugliandolo Serena Silvestro Guya Diletta Marconi Bruna Sinjari Jacopo Vecchiet Francesco Cipollone Damiano D’Ardes Antonio Auricchio Manuela Lanzini Sergio Caputi Rossella D’Aloisio Emanuela Mazzon Oriana Trubiani
author_facet	Leonardo Mastropasqua Lisa Toto Luigi Chiricosta Francesca Diomede Agnese Gugliandolo Serena Silvestro Guya Diletta Marconi Bruna Sinjari Jacopo Vecchiet Francesco Cipollone Damiano D’Ardes Antonio Auricchio Manuela Lanzini Sergio Caputi Rossella D’Aloisio Emanuela Mazzon Oriana Trubiani
author_sort	Leonardo Mastropasqua
title	Transcriptomic analysis revealed increased expression of genes involved in keratinization in the tears of COVID-19 patients
title_short	Transcriptomic analysis revealed increased expression of genes involved in keratinization in the tears of COVID-19 patients
title_full	Transcriptomic analysis revealed increased expression of genes involved in keratinization in the tears of COVID-19 patients
title_fullStr	Transcriptomic analysis revealed increased expression of genes involved in keratinization in the tears of COVID-19 patients
title_full_unstemmed	Transcriptomic analysis revealed increased expression of genes involved in keratinization in the tears of COVID-19 patients
title_sort	transcriptomic analysis revealed increased expression of genes involved in keratinization in the tears of covid-19 patients
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/0de353236beb40a9b301cdf412eaa96e
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Transcriptomic analysis revealed increased expression of genes involved in keratinization in the tears of COVID-19 patients

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