In vivo Molecular Imaging of Glutamate Carboxypeptidase II Expression in Re-endothelialisation after Percutaneous Balloon Denudation in a Rat Model

Abstract The short- and long-term success of intravascular stents depends on a proper re-endothelialisation after the intervention-induced endothelial denudation. The aim of this study was to evaluate the potential of in vivo molecular imaging of glutamate carboxypeptidase II (GCPII; identical with...

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Autores principales:	Heike Endepols, Felix M. Mottaghy, Sakine Simsekyilmaz, Jan Bucerius, Felix Vogt, Oliver Winz, Raphael Richarz, Philipp Krapf, Bernd Neumaier, Boris D. Zlatopolskiy, Agnieszka Morgenroth
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/0ded07f50df94a769f2016a9245a7218
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spelling	oai:doaj.org-article:0ded07f50df94a769f2016a9245a72182021-12-02T12:32:36ZIn vivo Molecular Imaging of Glutamate Carboxypeptidase II Expression in Re-endothelialisation after Percutaneous Balloon Denudation in a Rat Model10.1038/s41598-018-25863-12045-2322https://doaj.org/article/0ded07f50df94a769f2016a9245a72182018-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-25863-1https://doaj.org/toc/2045-2322Abstract The short- and long-term success of intravascular stents depends on a proper re-endothelialisation after the intervention-induced endothelial denudation. The aim of this study was to evaluate the potential of in vivo molecular imaging of glutamate carboxypeptidase II (GCPII; identical with prostate-specific membrane antigen PSMA) expression as a marker of re-endothelialisation. Fifteen Sprague Dawley rats underwent unilateral balloon angioplasty of the common carotid artery (CCA). Positron emission tomography (PET) using the GCPII-targeting tracer [18F]DCFPyL was performed after 5–21 days (scan 60–120 min post injection). In two animals, the GCPII inhibitor PMPA (23 mg/kg BW) was added to the tracer solution. After PET, both CCAs were removed, dissected, and immunostained with the GCPII specific antibody YPSMA-1. Difference of GCPII expression between both CCAs was established by PCR analysis. [18F]DCFPyL uptake was significantly higher in the ipsilateral compared to the contralateral CCA with an ipsi-/contralateral ratio of 1.67 ± 0.39. PMPA blocked tracer binding. The selective expression of GCPII in endothelial cells of the treated CCA was confirmed by immunohistological staining. PCR analysis verified the site-specific GCPII expression. By using a molecular imaging marker of GCPII expression, we provide the first non-invasive in vivo delineation of re-endothelialisation after angioplasty.Heike EndepolsFelix M. MottaghySakine SimsekyilmazJan BuceriusFelix VogtOliver WinzRaphael RicharzPhilipp KrapfBernd NeumaierBoris D. ZlatopolskiyAgnieszka MorgenrothNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-7 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Heike Endepols Felix M. Mottaghy Sakine Simsekyilmaz Jan Bucerius Felix Vogt Oliver Winz Raphael Richarz Philipp Krapf Bernd Neumaier Boris D. Zlatopolskiy Agnieszka Morgenroth In vivo Molecular Imaging of Glutamate Carboxypeptidase II Expression in Re-endothelialisation after Percutaneous Balloon Denudation in a Rat Model
description	Abstract The short- and long-term success of intravascular stents depends on a proper re-endothelialisation after the intervention-induced endothelial denudation. The aim of this study was to evaluate the potential of in vivo molecular imaging of glutamate carboxypeptidase II (GCPII; identical with prostate-specific membrane antigen PSMA) expression as a marker of re-endothelialisation. Fifteen Sprague Dawley rats underwent unilateral balloon angioplasty of the common carotid artery (CCA). Positron emission tomography (PET) using the GCPII-targeting tracer [18F]DCFPyL was performed after 5–21 days (scan 60–120 min post injection). In two animals, the GCPII inhibitor PMPA (23 mg/kg BW) was added to the tracer solution. After PET, both CCAs were removed, dissected, and immunostained with the GCPII specific antibody YPSMA-1. Difference of GCPII expression between both CCAs was established by PCR analysis. [18F]DCFPyL uptake was significantly higher in the ipsilateral compared to the contralateral CCA with an ipsi-/contralateral ratio of 1.67 ± 0.39. PMPA blocked tracer binding. The selective expression of GCPII in endothelial cells of the treated CCA was confirmed by immunohistological staining. PCR analysis verified the site-specific GCPII expression. By using a molecular imaging marker of GCPII expression, we provide the first non-invasive in vivo delineation of re-endothelialisation after angioplasty.
format	article
author	Heike Endepols Felix M. Mottaghy Sakine Simsekyilmaz Jan Bucerius Felix Vogt Oliver Winz Raphael Richarz Philipp Krapf Bernd Neumaier Boris D. Zlatopolskiy Agnieszka Morgenroth
author_facet	Heike Endepols Felix M. Mottaghy Sakine Simsekyilmaz Jan Bucerius Felix Vogt Oliver Winz Raphael Richarz Philipp Krapf Bernd Neumaier Boris D. Zlatopolskiy Agnieszka Morgenroth
author_sort	Heike Endepols
title	In vivo Molecular Imaging of Glutamate Carboxypeptidase II Expression in Re-endothelialisation after Percutaneous Balloon Denudation in a Rat Model
title_short	In vivo Molecular Imaging of Glutamate Carboxypeptidase II Expression in Re-endothelialisation after Percutaneous Balloon Denudation in a Rat Model
title_full	In vivo Molecular Imaging of Glutamate Carboxypeptidase II Expression in Re-endothelialisation after Percutaneous Balloon Denudation in a Rat Model
title_fullStr	In vivo Molecular Imaging of Glutamate Carboxypeptidase II Expression in Re-endothelialisation after Percutaneous Balloon Denudation in a Rat Model
title_full_unstemmed	In vivo Molecular Imaging of Glutamate Carboxypeptidase II Expression in Re-endothelialisation after Percutaneous Balloon Denudation in a Rat Model
title_sort	in vivo molecular imaging of glutamate carboxypeptidase ii expression in re-endothelialisation after percutaneous balloon denudation in a rat model
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/0ded07f50df94a769f2016a9245a7218
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In vivo Molecular Imaging of Glutamate Carboxypeptidase II Expression in Re-endothelialisation after Percutaneous Balloon Denudation in a Rat Model

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