Xuanfei Pingchuan Capsules Ameliorate Autophagy in Human Bronchial Epithelial Cells by Inhibiting p38 Phosphorylation

Background: This study aimed to investigate the protective effect of Xuanfei Pingchuan Capsules (XFPC) on autophagy and p38 phosphorylation in human bronchial epithelial (HBE) cells induced by cigarette smoke extract (CSE).Methods: HBE cells were divided into five groups: blank, CSE, low XFPC dose (...

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Autores principales:	Xiaoming Xue, Lihong Meng, Hongyu Cai, Yaoqin Sun, Ye Zhang, Hao Li, Yu Kang, Bobo Zhou, Fang Shang, Wei Guan, Li Zhang, Xu Chen, Luodan Zhang
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	chronic obstructive pulmonary disease Xuanfei Pingchuan capsules cigarette smoke extract human bronchial epithelial cells p38 phosphorylation autophagy Therapeutics. Pharmacology RM1-950
Acceso en línea:	https://doaj.org/article/0e00ed5db1ca48d38f564ec02a3ac08f
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spelling	oai:doaj.org-article:0e00ed5db1ca48d38f564ec02a3ac08f2021-12-02T11:42:50ZXuanfei Pingchuan Capsules Ameliorate Autophagy in Human Bronchial Epithelial Cells by Inhibiting p38 Phosphorylation1663-981210.3389/fphar.2021.748234https://doaj.org/article/0e00ed5db1ca48d38f564ec02a3ac08f2021-12-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fphar.2021.748234/fullhttps://doaj.org/toc/1663-9812Background: This study aimed to investigate the protective effect of Xuanfei Pingchuan Capsules (XFPC) on autophagy and p38 phosphorylation in human bronchial epithelial (HBE) cells induced by cigarette smoke extract (CSE).Methods: HBE cells were divided into five groups: blank, CSE, low XFPC dose (XFPC-L), medium XFPC dose (XFPC-M), and high XFPC dose (XFPC-H). HBE cells were induced by CSE to establish a cell model for chronic obstructive pulmonary disease, and different doses of XFPC medicated serum were used to treat the cells. The Cell Counting Kit-8 was used to detect cell viability. Flow cytometry was used to detect cell apoptosis. Fluorescence microscopy and the expression level of microtubule-associated protein light chain 3 (LC3)-II in immunohistochemical method were used to observe autophagy in cells. Western blot was used to detect the protein expression level of p38, phospho-p38 (p-p38), LC3-I, LC3-II and Beclin 1. Real-time polymerase chain reaction was used to detect the expression of LC3-I, LC3-II and Beclin 1 on mRNA level.Results: Compared with the blank group, the cell viability of the CSE group was significantly decreased, and apoptosis and the level of autophagy in cells were significantly increased. The mRNA and protein expression of LC3-I, LC3-II, Beclin 1 and the protein level of p-p38 were significantly increased in the CSE-HBE cells. Compared to the CSE group, the different doses of XFPC medicated serum increased cell viability, decreased cell apoptosis, and inhibited mRNA and protein expression of LC3-I, LC3-II, Beclin 1 and protein level of p-p38. These results were especially observed in the group XFPC-H. After adding a p38 agonist, the therapeutic effect of XFPC on cell viability and autophagy was suppressed.Conclusion: XFPC significantly increased cell viability in a CSE-induced HBE cell model for chronic obstructive pulmonary disease through inhibiting the level of autophagy mediated by phosphorylation of p38.Xiaoming XueLihong MengHongyu CaiYaoqin SunYe ZhangHao LiYu KangBobo ZhouFang ShangWei GuanLi ZhangXu ChenLuodan ZhangFrontiers Media S.A.articlechronic obstructive pulmonary diseaseXuanfei Pingchuan capsulescigarette smoke extracthuman bronchial epithelial cellsp38 phosphorylationautophagyTherapeutics. PharmacologyRM1-950ENFrontiers in Pharmacology, Vol 12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	chronic obstructive pulmonary disease Xuanfei Pingchuan capsules cigarette smoke extract human bronchial epithelial cells p38 phosphorylation autophagy Therapeutics. Pharmacology RM1-950
spellingShingle	chronic obstructive pulmonary disease Xuanfei Pingchuan capsules cigarette smoke extract human bronchial epithelial cells p38 phosphorylation autophagy Therapeutics. Pharmacology RM1-950 Xiaoming Xue Lihong Meng Hongyu Cai Yaoqin Sun Ye Zhang Hao Li Yu Kang Bobo Zhou Fang Shang Wei Guan Li Zhang Xu Chen Luodan Zhang Xuanfei Pingchuan Capsules Ameliorate Autophagy in Human Bronchial Epithelial Cells by Inhibiting p38 Phosphorylation
description	Background: This study aimed to investigate the protective effect of Xuanfei Pingchuan Capsules (XFPC) on autophagy and p38 phosphorylation in human bronchial epithelial (HBE) cells induced by cigarette smoke extract (CSE).Methods: HBE cells were divided into five groups: blank, CSE, low XFPC dose (XFPC-L), medium XFPC dose (XFPC-M), and high XFPC dose (XFPC-H). HBE cells were induced by CSE to establish a cell model for chronic obstructive pulmonary disease, and different doses of XFPC medicated serum were used to treat the cells. The Cell Counting Kit-8 was used to detect cell viability. Flow cytometry was used to detect cell apoptosis. Fluorescence microscopy and the expression level of microtubule-associated protein light chain 3 (LC3)-II in immunohistochemical method were used to observe autophagy in cells. Western blot was used to detect the protein expression level of p38, phospho-p38 (p-p38), LC3-I, LC3-II and Beclin 1. Real-time polymerase chain reaction was used to detect the expression of LC3-I, LC3-II and Beclin 1 on mRNA level.Results: Compared with the blank group, the cell viability of the CSE group was significantly decreased, and apoptosis and the level of autophagy in cells were significantly increased. The mRNA and protein expression of LC3-I, LC3-II, Beclin 1 and the protein level of p-p38 were significantly increased in the CSE-HBE cells. Compared to the CSE group, the different doses of XFPC medicated serum increased cell viability, decreased cell apoptosis, and inhibited mRNA and protein expression of LC3-I, LC3-II, Beclin 1 and protein level of p-p38. These results were especially observed in the group XFPC-H. After adding a p38 agonist, the therapeutic effect of XFPC on cell viability and autophagy was suppressed.Conclusion: XFPC significantly increased cell viability in a CSE-induced HBE cell model for chronic obstructive pulmonary disease through inhibiting the level of autophagy mediated by phosphorylation of p38.
format	article
author	Xiaoming Xue Lihong Meng Hongyu Cai Yaoqin Sun Ye Zhang Hao Li Yu Kang Bobo Zhou Fang Shang Wei Guan Li Zhang Xu Chen Luodan Zhang
author_facet	Xiaoming Xue Lihong Meng Hongyu Cai Yaoqin Sun Ye Zhang Hao Li Yu Kang Bobo Zhou Fang Shang Wei Guan Li Zhang Xu Chen Luodan Zhang
author_sort	Xiaoming Xue
title	Xuanfei Pingchuan Capsules Ameliorate Autophagy in Human Bronchial Epithelial Cells by Inhibiting p38 Phosphorylation
title_short	Xuanfei Pingchuan Capsules Ameliorate Autophagy in Human Bronchial Epithelial Cells by Inhibiting p38 Phosphorylation
title_full	Xuanfei Pingchuan Capsules Ameliorate Autophagy in Human Bronchial Epithelial Cells by Inhibiting p38 Phosphorylation
title_fullStr	Xuanfei Pingchuan Capsules Ameliorate Autophagy in Human Bronchial Epithelial Cells by Inhibiting p38 Phosphorylation
title_full_unstemmed	Xuanfei Pingchuan Capsules Ameliorate Autophagy in Human Bronchial Epithelial Cells by Inhibiting p38 Phosphorylation
title_sort	xuanfei pingchuan capsules ameliorate autophagy in human bronchial epithelial cells by inhibiting p38 phosphorylation
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/0e00ed5db1ca48d38f564ec02a3ac08f
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Xuanfei Pingchuan Capsules Ameliorate Autophagy in Human Bronchial Epithelial Cells by Inhibiting p38 Phosphorylation

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