Urine miRNA signature as a potential non-invasive diagnostic and prognostic biomarker in cervical cancer

Abstract MicroRNAs as cancer biomarkers in serum, plasma, and other body fluids are often used but analysis of miRNA in urine is limited. We investigated the expression of selected miRNAs in the paired urine, serum, cervical scrape, and tumor tissue specimens from the women with cervical precancer a...

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Autores principales:	Mehreen Aftab, Satish S. Poojary, Vaishnavi Seshan, Sachin Kumar, Pallavi Agarwal, Simran Tandon, Vijay Zutshi, Bhudev C. Das
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/0e00f00f7f4e440b821fccf72c798529
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spelling	oai:doaj.org-article:0e00f00f7f4e440b821fccf72c7985292021-12-02T15:43:23ZUrine miRNA signature as a potential non-invasive diagnostic and prognostic biomarker in cervical cancer10.1038/s41598-021-89388-w2045-2322https://doaj.org/article/0e00f00f7f4e440b821fccf72c7985292021-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-89388-whttps://doaj.org/toc/2045-2322Abstract MicroRNAs as cancer biomarkers in serum, plasma, and other body fluids are often used but analysis of miRNA in urine is limited. We investigated the expression of selected miRNAs in the paired urine, serum, cervical scrape, and tumor tissue specimens from the women with cervical precancer and cancer with a view to identify if urine miRNAs could be used as reliable non-invasive biomarkers for an early diagnosis and prognosis of cervical cancer. Expression of three oncomiRs (miR-21, miR-199a, and miR-155-5p) and three tumor suppressors (miR-34a, miR-145, and miR-218) as selected by database search in cervical pre-cancer, cancer, and normal controls including cervical cancer cell lines were analyzed using qRT-PCR. The expression of miRNAs was correlated with various clinicopathological parameters, including HPV infection and survival outcome. We observed a significant overexpression of the oncomiRs and the downregulation of tumor suppressor miRNAs. A combination of miR-145-5p, miR-218-5p, and miR-34a-5p in urine yielded 100% sensitivity and 92.8% specificity in distinguishing precancer and cancer patients from healthy controls and it well correlates with those of serum and tumor tissues. The expression of miR-34a-5p and miR-218-5p were found to be independent prognostic factors for the overall survival of cervical cancer patients. We conclude that the evaluation of the above specific miRNA expression in non-invasive urine samples may serve as a reliable biomarker for early detection and prognosis of cervical cancer.Mehreen AftabSatish S. PoojaryVaishnavi SeshanSachin KumarPallavi AgarwalSimran TandonVijay ZutshiBhudev C. DasNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-13 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Mehreen Aftab Satish S. Poojary Vaishnavi Seshan Sachin Kumar Pallavi Agarwal Simran Tandon Vijay Zutshi Bhudev C. Das Urine miRNA signature as a potential non-invasive diagnostic and prognostic biomarker in cervical cancer
description	Abstract MicroRNAs as cancer biomarkers in serum, plasma, and other body fluids are often used but analysis of miRNA in urine is limited. We investigated the expression of selected miRNAs in the paired urine, serum, cervical scrape, and tumor tissue specimens from the women with cervical precancer and cancer with a view to identify if urine miRNAs could be used as reliable non-invasive biomarkers for an early diagnosis and prognosis of cervical cancer. Expression of three oncomiRs (miR-21, miR-199a, and miR-155-5p) and three tumor suppressors (miR-34a, miR-145, and miR-218) as selected by database search in cervical pre-cancer, cancer, and normal controls including cervical cancer cell lines were analyzed using qRT-PCR. The expression of miRNAs was correlated with various clinicopathological parameters, including HPV infection and survival outcome. We observed a significant overexpression of the oncomiRs and the downregulation of tumor suppressor miRNAs. A combination of miR-145-5p, miR-218-5p, and miR-34a-5p in urine yielded 100% sensitivity and 92.8% specificity in distinguishing precancer and cancer patients from healthy controls and it well correlates with those of serum and tumor tissues. The expression of miR-34a-5p and miR-218-5p were found to be independent prognostic factors for the overall survival of cervical cancer patients. We conclude that the evaluation of the above specific miRNA expression in non-invasive urine samples may serve as a reliable biomarker for early detection and prognosis of cervical cancer.
format	article
author	Mehreen Aftab Satish S. Poojary Vaishnavi Seshan Sachin Kumar Pallavi Agarwal Simran Tandon Vijay Zutshi Bhudev C. Das
author_facet	Mehreen Aftab Satish S. Poojary Vaishnavi Seshan Sachin Kumar Pallavi Agarwal Simran Tandon Vijay Zutshi Bhudev C. Das
author_sort	Mehreen Aftab
title	Urine miRNA signature as a potential non-invasive diagnostic and prognostic biomarker in cervical cancer
title_short	Urine miRNA signature as a potential non-invasive diagnostic and prognostic biomarker in cervical cancer
title_full	Urine miRNA signature as a potential non-invasive diagnostic and prognostic biomarker in cervical cancer
title_fullStr	Urine miRNA signature as a potential non-invasive diagnostic and prognostic biomarker in cervical cancer
title_full_unstemmed	Urine miRNA signature as a potential non-invasive diagnostic and prognostic biomarker in cervical cancer
title_sort	urine mirna signature as a potential non-invasive diagnostic and prognostic biomarker in cervical cancer
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/0e00f00f7f4e440b821fccf72c798529
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Urine miRNA signature as a potential non-invasive diagnostic and prognostic biomarker in cervical cancer

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