An Additively Manufactured Sample Holder to Measure the Controlled Release of Vancomycin from Collagen Laminates

The controlled release of antibiotics prevents the spread of pathogens and thereby improves healing processes in regenerative medicine. However, high concentrations may interfere with healing processes. It is therefore advantageous to use biodegradable materials for a controlled release. In particul...

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Autores principales:	Michelle Fiona Kilb, Yannik Moos, Stefanie Eckes, Joy Braun, Ulrike Ritz, Daniela Nickel, Katja Schmitz
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	collagen controlled release crosslinking vancomycin additively manufactured sample holder Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/0e0c4e0bd94c4e52b79722192dcaa30a
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spelling	oai:doaj.org-article:0e0c4e0bd94c4e52b79722192dcaa30a2021-11-25T16:50:34ZAn Additively Manufactured Sample Holder to Measure the Controlled Release of Vancomycin from Collagen Laminates10.3390/biomedicines91116682227-9059https://doaj.org/article/0e0c4e0bd94c4e52b79722192dcaa30a2021-11-01T00:00:00Zhttps://www.mdpi.com/2227-9059/9/11/1668https://doaj.org/toc/2227-9059The controlled release of antibiotics prevents the spread of pathogens and thereby improves healing processes in regenerative medicine. However, high concentrations may interfere with healing processes. It is therefore advantageous to use biodegradable materials for a controlled release. In particular, multilayer materials enable differential release at different surfaces. For this purpose, collagen sheets of different properties can be bonded by photochemical crosslinking. Here, we present the development and application of an easily accessible, additively manufactured sample holder to study the controlled release of vancomycin from modularly assembled collagen laminates in two directions. As proof-of-concept, we show that laminates of collagen sheets covalently linked by rose bengal and green light crosslinking (RGX) can be tightly inserted into the device without leakage from the upper to lower cavity. We used this sample holder to detect the release of vancomycin from symmetrically and asymmetrically loaded two-layer and three-layer collagen laminates into the upper and lower cavity of the sample holder. We show that these collagen laminates are characterized by a collagen type-dependent vancomycin release, enabling the control of antibiotic release profiles as well as the direction of antibiotic release.Michelle Fiona KilbYannik MoosStefanie EckesJoy BraunUlrike RitzDaniela NickelKatja SchmitzMDPI AGarticlecollagencontrolled releasecrosslinkingvancomycinadditively manufactured sample holderBiology (General)QH301-705.5ENBiomedicines, Vol 9, Iss 1668, p 1668 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	collagen controlled release crosslinking vancomycin additively manufactured sample holder Biology (General) QH301-705.5
spellingShingle	collagen controlled release crosslinking vancomycin additively manufactured sample holder Biology (General) QH301-705.5 Michelle Fiona Kilb Yannik Moos Stefanie Eckes Joy Braun Ulrike Ritz Daniela Nickel Katja Schmitz An Additively Manufactured Sample Holder to Measure the Controlled Release of Vancomycin from Collagen Laminates
description	The controlled release of antibiotics prevents the spread of pathogens and thereby improves healing processes in regenerative medicine. However, high concentrations may interfere with healing processes. It is therefore advantageous to use biodegradable materials for a controlled release. In particular, multilayer materials enable differential release at different surfaces. For this purpose, collagen sheets of different properties can be bonded by photochemical crosslinking. Here, we present the development and application of an easily accessible, additively manufactured sample holder to study the controlled release of vancomycin from modularly assembled collagen laminates in two directions. As proof-of-concept, we show that laminates of collagen sheets covalently linked by rose bengal and green light crosslinking (RGX) can be tightly inserted into the device without leakage from the upper to lower cavity. We used this sample holder to detect the release of vancomycin from symmetrically and asymmetrically loaded two-layer and three-layer collagen laminates into the upper and lower cavity of the sample holder. We show that these collagen laminates are characterized by a collagen type-dependent vancomycin release, enabling the control of antibiotic release profiles as well as the direction of antibiotic release.
format	article
author	Michelle Fiona Kilb Yannik Moos Stefanie Eckes Joy Braun Ulrike Ritz Daniela Nickel Katja Schmitz
author_facet	Michelle Fiona Kilb Yannik Moos Stefanie Eckes Joy Braun Ulrike Ritz Daniela Nickel Katja Schmitz
author_sort	Michelle Fiona Kilb
title	An Additively Manufactured Sample Holder to Measure the Controlled Release of Vancomycin from Collagen Laminates
title_short	An Additively Manufactured Sample Holder to Measure the Controlled Release of Vancomycin from Collagen Laminates
title_full	An Additively Manufactured Sample Holder to Measure the Controlled Release of Vancomycin from Collagen Laminates
title_fullStr	An Additively Manufactured Sample Holder to Measure the Controlled Release of Vancomycin from Collagen Laminates
title_full_unstemmed	An Additively Manufactured Sample Holder to Measure the Controlled Release of Vancomycin from Collagen Laminates
title_sort	additively manufactured sample holder to measure the controlled release of vancomycin from collagen laminates
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/0e0c4e0bd94c4e52b79722192dcaa30a
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An Additively Manufactured Sample Holder to Measure the Controlled Release of Vancomycin from Collagen Laminates

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